Imaging Forster Resonance Energy Transfer Measurements of Transmembrane Helix Interactions in Lipid Bilayers on a Solid Support†

Langmuir ◽  
2004 ◽  
Vol 20 (21) ◽  
pp. 9053-9060 ◽  
Author(s):  
Edwin Li ◽  
Kalina Hristova
Keyword(s):  
Energy Transfer ◽  
Lipid Bilayers ◽  
Transmembrane Helix ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Solid Support ◽  
Forster Resonance Energy Transfer ◽  
Förster Resonance Energy Transfer ◽  
Förster Resonance ◽  
Helix Interactions

scholarly journals A Förster resonance energy transfer (FRET) approach for enhancing fluorescence contrast in phase-separated membranes

2011 ◽  
Vol 89 (3) ◽  
pp. 423-432
Author(s):  
Daniel M. Carter Ramirez ◽  
Jason Ding ◽  
Jack Guan ◽  
Dusan Vobornik ◽  
Anna Carnini ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Lipid Bilayers ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluid Phase ◽  
Forster Resonance Energy Transfer ◽  
Förster Resonance Energy Transfer ◽  
Fluorescence Contrast ◽  
Förster Resonance ◽  
Fluid Phases

The partitioning of the dye-labeled lipid probe, NBD-DHPE (1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt)), was examined by fluorescence microscopy in phase-separated lipid bilayers with mixtures of coexisting liquid-ordered and fluid phases. This probe shows slightly higher fluorescence intensity in the ordered domains but undergoes a contrast reversal to give a more strongly fluorescent fluid phase in the presence of >0.2% Texas red-DHPE (TR-DHPE). The change in contrast is shown to result from Förster resonance energy transfer between the NBD donor and TR acceptor in the fluid phase, which has a TR concentration that is approximately 3 times higher than in the domains. An alternate approach using a nitroxide-substituted lipid that partitions into the fluid phase as a quencher, was also examined as a means to enhance the contrast; however, the quencher modified the behaviour of the bilayer. The energy transfer method for enhancing the contrast between ordered and fluid phases was used to examine the morphology of enzyme-treated bilayers.

Sign in / Sign up

Export Citation Format

Share Document