Tethered or Adsorbed Supported Lipid Bilayers in Nanotubes Characterized by Deuterium Magic Angle Spinning NMR Spectroscopy

Langmuir ◽  
2005 ◽  
Vol 21 (8) ◽  
pp. 3226-3228 ◽  
Author(s):  
Olivier Wattraint ◽  
Dror E. Warschawski ◽  
Catherine Sarazin
Keyword(s):  
Nmr Spectroscopy ◽  
Lipid Bilayers ◽  
Magic Angle Spinning ◽  
Supported Lipid Bilayers ◽  
Magic Angle ◽  
Angle Spinning

scholarly journals Ellagitannin–Lipid Interaction by HR-MAS NMR Spectroscopy

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 373
Author(s):  
Valtteri Virtanen ◽  
Susanna Räikkönen ◽  
Elina Puljula ◽  
Maarit Karonen
Keyword(s):  
Nmr Spectroscopy ◽  
Lipid Bilayers ◽  
Membrane Lipids ◽  
Nuclear Overhauser Effect ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Relaxation Rates ◽  
Lipid Mixture ◽  
Nuclear Overhauser ◽  
Angle Spinning

Ellagitannins have antimicrobial activity, which might be related to their interactions with membrane lipids. We studied the interactions of 12 different ellagitannins and pentagalloylglucose with a lipid extract of Escherichia coli by high-resolution magic angle spinning NMR spectroscopy. The nuclear Overhauser effect was utilized to measure the cross relaxation rates between ellagitannin and lipid protons. The shifting of lipid signals in 1H NMR spectra of ellagitannin–lipid mixture due to ring current effect was also observed. The ellagitannins that showed interaction with lipids had clear structural similarities. All ellagitannins that had interactions with lipids had glucopyranose cores. In addition to the central polyol, the most important structural feature affecting the interaction seemed to be the structural flexibility of the ellagitannin. Even dimeric and trimeric ellagitannins could penetrate to the lipid bilayers if their structures were flexible with free galloyl and hexahydroxydiphenoyl groups.

scholarly journals Magic-angle-spinning NMR spectroscopy applied to small molecules and peptides in lipid bilayers

2007 ◽  
Vol 35 (5) ◽  
pp. 991-995 ◽  
Author(s):  
C. Ader ◽  
R. Schneider ◽  
K. Seidel ◽  
M. Etzkorn ◽  
M. Baldus
Keyword(s):  
Nmr Spectroscopy ◽  
Small Molecules ◽  
Lipid Bilayers ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Domain Formation ◽  
Structure And Dynamics ◽  
Molecular Complexation ◽  
Angle Spinning ◽  
Insight Into

ssNMR (solid-state NMR) spectroscopy provides increasing possibilities to study the structural and dynamic aspects of biological membranes. Here, we review recent ssNMR experiments that are based on MAS (magic angle spinning) and that provide insight into the structure and dynamics of membrane systems at the atomic level. Such methods can be used to study membrane architecture, domain formation or molecular complexation in a way that is highly complementary to other biophysical methods such as imaging or calorimetry.

Wideline 2H -NMR Spectroscopy and Imaging of Solids

1994 ◽  
Vol 49 (1-2) ◽  
pp. 19-26 ◽  
Author(s):  
B. Blümich
Keyword(s):  
Nmr Spectroscopy ◽  
Excitation Power ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
2H Nmr ◽  
Stochastic Excitation ◽  
2H Nmr Spectroscopy ◽  
Spatially Inhomogeneous ◽  
Recent Developments ◽  
Angle Spinning

Abstract Recent developments, focussing on reduction of the rf excitation power by stochastic excitation, on improvements in sensitivity and excitation bandwidth by magic angle spinning, and on combining wideline spectroscopy with spatial resolution for investigations o f spatially inhomogeneous objects are reviewed.

scholarly journals Sampling Method Affects HR-MAS NMR Spectra of Healthy Caprine Brain Biopsies

Metabolites ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 38
Author(s):  
Annakatrin Häni ◽  
Gaëlle Diserens ◽  
Anna Oevermann ◽  
Peter Vermathen ◽  
Christina Precht
Keyword(s):  
Nmr Spectroscopy ◽  
Sampling Method ◽  
Magic Angle Spinning ◽  
Mas Nmr ◽  
Magic Angle ◽  
1H Nuclear Magnetic Resonance ◽  
Rapid Changes ◽  
Tissue Degradation ◽  
Angle Spinning

The metabolic profiling of tissue biopsies using high-resolution–magic angle spinning (HR-MAS) 1H nuclear magnetic resonance (NMR) spectroscopy may be influenced by experimental factors such as the sampling method. Therefore, we compared the effects of two different sampling methods on the metabolome of brain tissue obtained from the brainstem and thalamus of healthy goats by 1H HR-MAS NMR spectroscopy—in vivo-harvested biopsy by a minimally invasive stereotactic approach compared with postmortem-harvested sample by dissection with a scalpel. Lactate and creatine were elevated, and choline-containing compounds were altered in the postmortem compared to the in vivo-harvested samples, demonstrating rapid changes most likely due to sample ischemia. In addition, in the brainstem samples acetate and inositols, and in the thalamus samples ƴ-aminobutyric acid, were relatively increased postmortem, demonstrating regional differences in tissue degradation. In conclusion, in vivo-harvested brain biopsies show different metabolic alterations compared to postmortem-harvested samples, reflecting less tissue degradation. Sampling method and brain region should be taken into account in the analysis of metabolic profiles. To be as close as possible to the actual situation in the living individual, it is desirable to use brain samples obtained by stereotactic biopsy whenever possible.

Pulsed Field Gradient Selection in Two-Dimensional Magic Angle Spinning NMR Spectroscopy of Dipolar Solids

1998 ◽  
Vol 134 (2) ◽  
pp. 355-359 ◽  
Author(s):  
Tilo Fritzhanns ◽  
Siegfried Hafner ◽  
Dan E. Demco ◽  
Hans W. Spiess ◽  
Frank H. Laukien
Keyword(s):  
Nmr Spectroscopy ◽  
Field Gradient ◽  
Magic Angle Spinning ◽  
Magic Angle ◽  
Two Dimensional ◽  
Pulsed Field ◽  
Angle Spinning
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