Using the Adhesive Interaction between Atomic Force Microscopy Tips and Polymer Surfaces to Measure the Elastic Modulus of Compliant Samples

Langmuir ◽  
2004 ◽  
Vol 20 (14) ◽  
pp. 5837-5845 ◽  
Author(s):  
Yujie Sun ◽  
Boris Akhremitchev ◽  
Gilbert C. Walker
Keyword(s):  
Atomic Force Microscopy ◽  
Elastic Modulus ◽  
Polymer Surfaces ◽  
Adhesive Interaction ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Substrate Chemistry-Dependent Conformations of Single Laminin Molecules on Polymer Surfaces are Revealed by the Phase Signal of Atomic Force Microscopy

2007 ◽  
Vol 93 (1) ◽  
pp. 202-207 ◽  
Author(s):  
Jose Carlos Rodríguez Hernández ◽  
Manuel Salmerón Sánchez ◽  
José Miguel Soria ◽  
José Luis Gómez Ribelles ◽  
Manuel Monleón Pradas
Keyword(s):  
Atomic Force Microscopy ◽  
Polymer Surfaces ◽  
Force Microscopy ◽  
Atomic Force ◽  
Phase Signal

Elastic Modulus of Single Cellulose Microfibrils from Tunicate Measured by Atomic Force Microscopy

2009 ◽  
Vol 10 (9) ◽  
pp. 2571-2576 ◽  
Author(s):  
Shinichiro Iwamoto ◽  
Weihua Kai ◽  
Akira Isogai ◽  
Tadahisa Iwata
Keyword(s):  
Atomic Force Microscopy ◽  
Elastic Modulus ◽  
Cellulose Microfibrils ◽  
Force Microscopy ◽  
Atomic Force

Quantitative mechanical analysis of indentations on layered, soft elastic materials

Soft Matter ◽  
2019 ◽  
Vol 15 (8) ◽  
pp. 1776-1784 ◽  
Author(s):  
Bryant L. Doss ◽  
Kiarash Rahmani Eliato ◽  
Keng-hui Lin ◽  
Robert Ros
Keyword(s):  
Atomic Force Microscopy ◽  
Elastic Modulus ◽  
Cell Mechanics ◽  
Biological Samples ◽  
Mechanical Analysis ◽  
Elastic Materials ◽  
Mechanical Heterogeneity ◽  
Popular Method ◽  
Force Microscopy ◽  
Atomic Force

Atomic force microscopy (AFM) is becoming an increasingly popular method for studying cell mechanics, however the existing analysis tools for determining the elastic modulus from indentation experiments are unable to quantitatively account for mechanical heterogeneity commonly found in biological samples.

scholarly journals An investigation of surface properties, local elastic modulus and interaction with simulated pulmonary surfactant of surface modified inhalable voriconazole dry powders using atomic force microscopy

RSC Advances ◽  
2016 ◽  
Vol 6 (31) ◽  
pp. 25789-25798 ◽  
Author(s):  
Sumit Arora ◽  
Michael Kappl ◽  
Mehra Haghi ◽  
Paul M. Young ◽  
Daniela Traini ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Elastic Modulus ◽  
Surface Properties ◽  
Pulmonary Surfactant ◽  
Dry Powders ◽  
Force Microscopy ◽  
Atomic Force ◽  
Surface Modified ◽  
Spray Dried

l-Leucine modified voriconazole spray dried micropartcles.

scholarly journals Stiffness and heterogeneity of the pulmonary endothelial glycocalyx measured by atomic force microscopy

2011 ◽  
Vol 301 (3) ◽  
pp. L353-L360 ◽  
Author(s):  
Ryan O'Callaghan ◽  
Kathleen M. Job ◽  
Randal O. Dull ◽  
Vladimir Hlady
Keyword(s):  
Atomic Force Microscopy ◽  
Elastic Modulus ◽  
Indentation Depth ◽  
Cell Junctions ◽  
Endothelial Glycocalyx ◽  
Cell Junction ◽  
Microvascular Endothelial Cell ◽  
Cell Contact ◽  
Force Microscopy ◽  
Atomic Force

The mechanical properties of endothelial glycocalyx were studied using atomic force microscopy with a silica bead (diameter ∼18 μm) serving as an indenter. Even at indentations of several hundred nanometers, the bead exerted very low compressive pressures on the bovine lung microvascular endothelial cell (BLMVEC) glycocalyx and allowed for an averaging of stiffness in the bead-cell contact area. The elastic modulus of BLMVEC glycocalyx was determined as a pointwise function of the indentation depth before and after enzymatic degradation of specific glycocalyx components. The modulus-indentation depth profiles showed the cells becoming progressively stiffer with increased indentation. Three different enzymes were used: heparinases III and I and hyaluronidase. The main effects of heparinase III and hyaluronidase enzymes were that the elastic modulus in the cell junction regions increased more rapidly with the indentation than in BLMVEC controls, and that the effective thickness of glycocalyx was reduced. Cytochalasin D abolished the modulus increase with the indentation. The confocal profiling of heparan sulfate and hyaluronan with atomic force microscopy indentation data demonstrated marked heterogeneity of the glycocalyx composition between cell junctions and nuclear regions.

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