Controlling Cell Adhesion on Human Tissue by Soft Lithography

Langmuir ◽  
2004 ◽  
Vol 20 (10) ◽  
pp. 4155-4161 ◽  
Author(s):  
Christina J. Lee ◽  
Mark S. Blumenkranz ◽  
Harvey A. Fishman ◽  
Stacey F. Bent
Keyword(s):  
Cell Adhesion ◽  
Human Tissue ◽  
Soft Lithography

scholarly journals Fabrication of Piezoelectric Polyvinylidene Fluoride (PVDF) Microstructures by Soft Lithography for Tissue Engineering and Cell Biology Applications

2007 ◽  
Vol 1002 ◽  
Author(s):  
Daniel Gallego ◽  
Nicholas J. Ferrell ◽  
Derek J. Hansford
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Soft Lithography ◽  
Polyvinylidene Fluoride ◽  
Tissue Engineering Scaffolds ◽  
Human Osteosarcoma ◽  
Aspect Ratios ◽  
Parallel Lines

ABSTRACTA method for the fabrication of piezoelectric polyvinylidene fluoride (PVDF) microstructures is described. Embossed and individual features with highly defined geometries at the microscale were obtained using soft lithography-based techniques. Various structure geometries were obtained, including pillars (three different aspect ratios), parallel lines, and criss-crossed lines. SEM characterization revealed uniform patterns with dimensions ranging from 2 μm ñ 15 μm. Human osteosarcoma (HOS) cell cultures were used to evaluate the cytocompatibility of the microstructures. SEM and fluorescence microscopy showed adequate cell adhesion, proliferation, and strong interaction with tips and corners of the microdiscontinuities. Microfabricated piezoelectric PVDF structures could find applications in the fabrication of mechanically active tissue engineering scaffolds, and the development of dynamic sensors at the cellular and subcellular levels.

scholarly journals Fabrication and Hydrodynamic Characterization of a Microfluidic Device for Cell Adhesion Tests in Polymeric Surfaces

Micromachines ◽  
2019 ◽  
Vol 10 (5) ◽  
pp. 303 ◽  
Author(s):  
J. Ponmozhi ◽  
J. M. R. Moreira ◽  
F. J. Mergulhão ◽  
J. B. L. M. Campos ◽  
J. M. Miranda
Keyword(s):  
Cell Adhesion ◽  
Microfluidic Device ◽  
Bacterial Adhesion ◽  
Soft Lithography ◽  
Region Of Interest ◽  
Polymeric Materials ◽  
Fabrication Method ◽  
Major Drawback ◽  
Polymeric Surfaces ◽  
Rectangular Channels

A fabrication method is developed to produce a microfluidic device to test cell adhesion to polymeric materials. The process is able to produce channels with walls of any spin coatable polymer. The method is a modification of the existing poly-dimethylsiloxane soft lithography method and, therefore, it is compatible with sealing methods and equipment of most microfluidic laboratories. The molds are produced by xurography, simplifying the fabrication in laboratories without sophisticated equipment for photolithography. The fabrication method is tested by determining the effective differences in bacterial adhesion in five different materials. These materials have different surface hydrophobicities and charges. The major drawback of the method is the location of the region of interest in a lowered surface. It is demonstrated by bacterial adhesion experiments that this drawback has a negligible effect on adhesion. The flow in the device was characterized by computational fluid dynamics and it was shown that shear stress in the region of interest can be calculated by numerical methods and by an analytical equation for rectangular channels. The device is therefore validated for adhesion tests.

Tissue-specific distributions of alternatively spliced human PECAM-1 isoforms

2003 ◽  
Vol 284 (3) ◽  
pp. H1008-H1017 ◽  
Author(s):  
Yongji Wang ◽  
Xiaojing Su ◽  
Christine M. Sorenson ◽  
Nader Sheibani
Keyword(s):  
Endothelial Cells ◽  
Cell Adhesion ◽  
Alternative Splicing ◽  
Endothelial Cell ◽  
Adhesion Molecule ◽  
Human Tissue ◽  
Cell Adhesion Molecule ◽  
Cytoplasmic Domain ◽  
Full Length ◽  
Pcr Cloning

Platelet endothelial cell adhesion molecule-1 (PECAM-1) is a cell adhesion molecule that is highly expressed on the surface of endothelial cells and some hematopoietic cells. Its cytoplasmic domain is encoded by multiple exons, which undergo alternative splicing. Here, we demonstrate that the human PECAM-1 cytoplasmic domain undergoes alternative splicing, generating six different isoforms. RT-PCR cloning and DNA sequence analysis indicated that human tissue and endothelial cells express multiple isoforms of PECAM-1, including the full-length PECAM-1 and five other isoforms, which lack exon 12, 13, 14, or 15 or exons 14 and 15. The full-length PECAM-1 is the predominant isoform detected in human tissue and endothelial cells. This is in contrast to murine endothelium, in which the PECAM-1 isoform lacking exons 14 and 15 is the predominant isoform. The PECAM-1 isoform lacking exon 13 detected in human tissue and endothelial cells is absent in murine endothelium. The expression pattern of PECAM-1 isoforms changes during tube formation of endothelial cells on Matrigel, which may indicate specialized roles for specific isoforms of PECAM-1 during angiogenesis. The data presented here demonstrate that human PECAM-1 undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues. Therefore, the regulated expression of these isoforms may influence endothelial cell adhesive properties during angiogenesis and/or vasculogenesis.

622: Von Hippel-Lindau Inactivation Downregulates the Cell-Cell Adhesion Molecule E Cadherin in Renal Cancer Cells

2005 ◽  
Vol 173 (4S) ◽  
pp. 170-170
Author(s):  
Maxine G. Tran ◽  
Miguel A. Esteban ◽  
Peter D. Hill ◽  
Ashish Chandra ◽  
Tim S. O'Brien ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cancer Cells ◽  
Renal Cancer ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Von Hippel Lindau ◽  
E Cadherin ◽  
Cell Cell
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