Use of the Atomic Force Microscope To Determine the Effect of Substratum Surface Topography on Bacterial Adhesion

Langmuir ◽  
2002 ◽  
Vol 18 (6) ◽  
pp. 2343-2346 ◽  
Author(s):  
R. D. Boyd ◽  
J. Verran ◽  
M. V. Jones ◽  
M. Bhakoo
Keyword(s):  
Atomic Force Microscope ◽  
Surface Topography ◽  
Bacterial Adhesion ◽  
Atomic Force

Quantification of Axonal Outgrowth on a Surface with Asymmetric Topography

2014 ◽  
Vol 1621 ◽  
pp. 243-248
Author(s):  
Elise Spedden ◽  
Cristian Staii
Keyword(s):  
Atomic Force Microscope ◽  
Surface Topography ◽  
Nerve Regeneration ◽  
Growth Cone ◽  
Effective Potential ◽  
Axonal Outgrowth ◽  
Directional Bias ◽  
Atomic Force ◽  
Neuronal Growth Cone ◽  
Neuron Growth

ABSTRACTTopographical features are known to influence the axonal outgrowth of neurons. Understanding what kinds of topographical features are most effective at growth cone guidance and how outgrowth responds to these structures is of great importance to the study of nerve regeneration. To this end we analyze axonal outgrowth on tilted nanorod substrates which have been shown to impart directional bias to neuron growth. We utilize the Atomic Force Microscope to characterize the surface features present on these substrates and how such features are influencing the axonal outgrowth. Additionally, using a model which considers the neuronal growth cone as an object influenced by an effective potential we determine an effective force imparted on the growth cone by the surface topography.

scholarly journals Contributions of lunate cells and wax crystals to the surface anisotropy of Nepenthes slippery zone

2018 ◽  
Vol 5 (9) ◽  
pp. 180766 ◽  
Author(s):  
Lixin Wang ◽  
Dashuai Tao ◽  
Shiyun Dong ◽  
Shanshan Li ◽  
Yu Tian
Keyword(s):  
Atomic Force Microscope ◽  
Surface Topography ◽  
Friction Force ◽  
Body Length ◽  
Structural Features ◽  
Large Displacements ◽  
Surface Anisotropy ◽  
Atomic Force ◽  
Microstructure Examination ◽  
Wax Crystals

Nepenthes slippery zone presents surface anisotropy depending on its specialized structures. Herein, via macro–micro–nano scaled experiments, we analysed the contributions of lunate cells and wax crystals to this anisotropy. Macroscopic climbing of insects showed large displacements (triple body length within 3 s) and high velocities (6.16–20.47 mm s −1 ) in the inverted-fixed (towards digestive zone) slippery zone, but failed to climb forward in the normal-fixed (towards peristome) one. Friction force of insect claws sliding across inverted-fixed lunate cells was about 2.4 times of that sliding across the normal-fixed ones, whereas showed unobvious differences (1.06–1.11 times) between the inverted- and normal-fixed wax crystals. Innovative results from atomic force microscope scanning and microstructure examination demonstrated the upper layer of wax crystals causes the cantilever tip to generate rather small differences in friction data (1.92–2.72%), and the beneath layer provides slightly higher differences (4.96–7.91%). The study confirms the anisotropic configuration of lunate cells produces most of the anisotropy, whereas both surface topography and structural features of the wax crystals generate a slight contribution. These results are helpful for understanding the surface anisotropy of Nepenthes slippery zone, and guide the design of bioinspired surface with anisotropic properties.

Quantifying bacterial adhesion on antifouling polymer brushes via single-cell force spectroscopy

2015 ◽  
Vol 6 (31) ◽  
pp. 5740-5751 ◽  
Author(s):  
Cesar Rodriguez-Emmenegger ◽  
Sébastien Janel ◽  
Andres de los Santos Pereira ◽  
Michael Bruns ◽  
Frank Lafont
Keyword(s):  
Atomic Force Microscope ◽  
Single Cell ◽  
Bacterial Adhesion ◽  
Bacterial Cell ◽  
Polymer Brushes ◽  
Force Spectroscopy ◽  
Adhesion Forces ◽  
Atomic Force ◽  
Cell Force

The adhesion forces between a single bacterial cell and different polymer brushes were measured directly with an atomic force microscope and correlated with their resistance to fouling.

Atomic Force and Ultrasonic Force Microscopic Surface Characterization of Laser Treated Head Sliders

2001 ◽  
Author(s):  
Carl Druffner ◽  
Edward J. Schumaker ◽  
Liming Shen ◽  
Shamachary Sathish ◽  
Ganesh N. Raikar ◽  
...  
Keyword(s):  
Atomic Force Microscope ◽  
Surface Topography ◽  
Surface Characterization ◽  
Atomic Force ◽  
Hard Disc Drive ◽  
Microscope Imaging ◽  
Disc Drive ◽  
Microscopic Surface

Abstract The surface of laser treated hard disc drive head sliders has been imaged using the Atomic Force Microscope and Ultrasonic Force Microscope. The contrast of the surface topography image from the Atomic Force Microscope is compared with the elasticity image generated by the Ultrasonic Force Microscope on the same sample region. Images of microcracking in the laser treated regions are presented. The possible reasons for the development of microcracking and the enhanced contrast of the Ultrasonic Force Microscope imaging of these microcracks are discussed.

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