The Role of Specific Binding in Human Serum Albumin Adsorption to Self-Assembled Monolayers

Langmuir ◽  
2002 ◽  
Vol 18 (2) ◽  
pp. 557-561 ◽  
Author(s):  
Eugene J. Choi ◽  
Mark D. Foster
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Specific Binding ◽  
Self Assembled Monolayers ◽  
Assembled Monolayers ◽  
Self Assembled ◽  
Albumin Adsorption

Neutron and X-ray Reflectivity Studies of Human Serum Albumin Adsorption onto Functionalized Surfaces of Self-Assembled Monolayers

1997 ◽  
Vol 13 (5) ◽  
pp. 635-639 ◽  
Author(s):  
S. Petrash ◽  
A. Liebmann-Vinson ◽  
M.D. Foster ◽  
L.M. Lander ◽  
W.J. Brittain ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Self Assembled Monolayers ◽  
X Ray ◽  
Functionalized Surfaces ◽  
Assembled Monolayers ◽  
Self Assembled ◽  
Albumin Adsorption

Effect of Flow on Human Serum Albumin Adsorption to Self-Assembled Monolayers of Varying Packing Density

Langmuir ◽  
2003 ◽  
Vol 19 (13) ◽  
pp. 5464-5474 ◽  
Author(s):  
Eugene J. Choi ◽  
Mark D. Foster ◽  
Susan Daly ◽  
Robert Tilton ◽  
Todd Przybycien ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Packing Density ◽  
Self Assembled Monolayers ◽  
Assembled Monolayers ◽  
Self Assembled ◽  
Albumin Adsorption

Changes in Protein Adsorption on Self-Assembled Monolayers with Monolayer Order:  Comparison of Human Serum Albumin and Human Gamma Globulin

Langmuir ◽  
2001 ◽  
Vol 17 (24) ◽  
pp. 7645-7651 ◽  
Author(s):  
Stanislaw Petrash ◽  
Tricia Cregger ◽  
Bin Zhao ◽  
Elena Pokidysheva ◽  
Mark D. Foster ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Protein Adsorption ◽  
Gamma Globulin ◽  
Self Assembled Monolayers ◽  
Assembled Monolayers ◽  
Self Assembled ◽  
Human Gamma Globulin ◽  
Order Comparison

Porphyrin self-assembled monolayers and photodynamic oxidation of tryptophan

2010 ◽  
Vol 14 (01) ◽  
pp. 81-88 ◽  
Author(s):  
Isabelle Chambrier ◽  
David A. Russell ◽  
Derek E. Brundish ◽  
William G. Love ◽  
Giulio Jori ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Fluorescence Spectroscopy ◽  
Serum Albumin ◽  
White Light ◽  
Self Assembled Monolayers ◽  
Self Assembled Monolayer ◽  
Glass Substrates ◽  
Assembled Monolayers ◽  
Self Assembled ◽  
Derivatives Of

The zinc and magnesium metalated derivatives of 5,5′-[12,12′-di(thiododecyloxy)-4,4′-phenyl)]-10,10′,15,15′,20,20′-hexakis(3,3′,4,4′,5,5′-hexakisdecyloxyphenyl)diporphyrin, 1b and 1c, have been synthesised and deposited to form self-assembled monolayer (SAM) films on the surface of gold-coated glass substrates. The SAM films have been characterized by RAIR spectroscopy and fluorescence spectroscopy. The potential for the porphyrin films to catalyze the oxidation of tryptophan within human serum albumin upon irradiation with white light has been demonstrated and attributed to the porphyrins acting as photosensitizers of oxygen to form oxidizing species.

scholarly journals Porous Aluminium Oxide Coating for the Development of Spectroscopic Ellipsometry Based Biosensor: Evaluation of Human Serum Albumin Adsorption

Coatings ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1018
Author(s):  
Vincentas Maciulis ◽  
Uldis Malinovskis ◽  
Donats Erts ◽  
Arunas Ramanavicius ◽  
Almira Ramanaviciene ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Spectroscopic Ellipsometry ◽  
Specific Binding ◽  
Aluminium Oxide ◽  
Oxide Coating ◽  
Buffer Solution ◽  
Sem Images ◽  
Albumin Adsorption

An electrochemically synthesised porous anodic aluminium oxide (pAAO) layer has been analysed by means of spectroscopic ellipsometry. The determined thickness of the formed pAAO layer obtained from spectroscopic ellipsometry measurements and modelling was 322.75 ± 0.12 nm. The radius of the nanopores estimated from SEM images was 39 ± 5 nm and the distance between nanopores was 107 ± 6 nm. The investigation of human serum albumin (HSA) adsorption on the pAAO coating showed that: (i) the protein concentration inside nanopores, depending on exposure time, approximately was from 200 up to 600 times higher than that determined in buffer solution; (ii) the initial phase of the adsorption process is slow (3.23 mg·cm−3·min−1) in comparison with the protein desorption rate (21.2 mg·cm−3·min−1) by means of pAAO layer washing; (iii) conventional washing with PBS solution and deionised water does not completely remove HSA molecules from pAAO pores and, therefore, the HSA concentration inside nanopores after 16 h of washing still remains almost 100 times higher than that present in PBS solution. Thus, due to such binding ability, HSA can be successfully used for the blocking of the remaining free surface, which is applied for the reduction in non-specific binding after the immobilisation of biorecognition molecules on the pAAO surface. It was determined that some desorption of HSA molecules from the pAAO layer occurred during the sensor’s surface washing step; however, HSA concentration inside the nanopores still remained rather high. These results recommend the continued application of pAAO in the development of biosensors.

scholarly journals Microfluidic Impedance Biosensor Chips Using Sensing Layers Based on DNA-Based Self-Assembled Monolayers for Label-Free Detection of Proteins

Biosensors ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 80
Author(s):  
Khaled Alsabbagh ◽  
Tim Hornung ◽  
Achim Voigt ◽  
Sahba Sadir ◽  
Taleieh Rajabi ◽  
...  
Keyword(s):  
Troponin I ◽  
Electrochemical Impedance ◽  
Specific Binding ◽  
Self Assembled Monolayers ◽  
Significant Shift ◽  
Label Free ◽  
Label Free Detection ◽  
Assembled Monolayers ◽  
Self Assembled ◽  
Biosensor Chip

A microfluidic chip for electrochemical impedance spectroscopy (EIS) is presented as bio-sensor for label-free detection of proteins by using the example of cardiac troponin I. Troponin I is one of the most specific diagnostic serum biomarkers for myocardial infarction. The microfluidic impedance biosensor chip presented here consists of a microscope glass slide serving as base plate, sputtered electrodes, and a polydimethylsiloxane (PDMS) microchannel. Electrode functionalization protocols were developed considering a possible charge transfer through the sensing layer, in addition to analyte-specific binding by corresponding antibodies and reduction of nonspecific protein adsorption to prevent false-positive signals. Reagents tested for self-assembled monolayers (SAMs) on gold electrodes included thiolated hydrocarbons and thiolated oligonucleotides, where SAMs based on the latter showed a better performance. The corresponding antibody was covalently coupled on the SAM using carbodiimide chemistry. Sampling and measurement took only a few minutes. Application of a human serum albumin (HSA) sample, 1000 ng/mL, led to negligible impedance changes, while application of a troponin I sample, 1 ng/mL, led to a significant shift in the Nyquist plot. The results are promising regarding specific detection of clinically relevant concentrations of biomarkers, such as cardiac markers, with the newly developed microfluidic impedance biosensor chip.

scholarly journals Mechanical and Charge Transport Properties of Alkanethiol Self-Assembled Monolayers on a Au(111) Surface:  The Role of Molecular Tilt

Langmuir ◽  
2008 ◽  
Vol 24 (5) ◽  
pp. 2219-2223 ◽  
Author(s):  
Yabing Qi ◽  
Imma Ratera ◽  
Jeong Y. Park ◽  
Paul D. Ashby ◽  
Su Ying Quek ◽  
...  
Keyword(s):  
Charge Transport ◽  
Transport Properties ◽  
Self Assembled Monolayers ◽  
Assembled Monolayers ◽  
Self Assembled
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