Model biological microemulsions. 2. Water-(cholesteryl benzoate+heptane)-Triton X-100 butanol microemulsions containing dextran, gelatin, bovine serum albumin, and sodium chloride

Langmuir ◽  
1991 ◽  
Vol 7 (4) ◽  
pp. 636-642 ◽  
Author(s):  
M. L. Das ◽  
P. K. Bhattacharya ◽  
S. P. Moulik
Keyword(s):  
Sodium Chloride ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Triton X

Binding of the triton X series of nonionic surfactants to bovine serum albumin

Biochemistry ◽  
1980 ◽  
Vol 19 (5) ◽  
pp. 912-917 ◽  
Author(s):  
Wayne W. Sukow ◽  
Howard E. Sandberg ◽  
Edwin A. Lewis ◽  
Delbert J. Eatough ◽  
Lee D. Hansen
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Nonionic Surfactants ◽  
Triton X

On the control of crystal growth in bovine serum albumin-sodium chloride thin film gels

1999 ◽  
Vol 2 (1) ◽  
pp. 79-89 ◽  
Author(s):  
Céline Annarelli ◽  
Laurence Reyes ◽  
Jean Fornazero ◽  
Jacques Bert ◽  
Richard Cohen ◽  
...  
Keyword(s):  
Thin Film ◽  
Crystal Growth ◽  
Sodium Chloride ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum

scholarly journals Formation of Nanocomplexes between Carboxymethyl Inulin and Bovine Serum Albumin via pH-Induced Electrostatic Interaction

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3056 ◽  
Author(s):  
Guiying Huang ◽  
Jun Liu ◽  
Weiping Jin ◽  
Zihao Wei ◽  
Chi-Tang Ho ◽  
...  
Keyword(s):  
Sodium Chloride ◽  
Bovine Serum Albumin ◽  
Complex Formation ◽  
Serum Albumin ◽  
Electrostatic Interaction ◽  
Bovine Serum ◽  
Titration Calorimetry ◽  
Food Ingredients ◽  
Enthalpy Changes ◽  
Ft Ir

As a functional polysaccharide, inulin was carboxymethylated and it formed nanocomplexes with bovine serum albumin (BSA). The success of obtaining carboxymethyl inulin (CMI) was confirmed by a combination of Fourier transform Infrared (FT-IR), Raman spectroscopy, gel permeation chromatography (GPC), and titration. The effects of pH and ionic strength on the formation of CMI/BSA nanocomplexes were investigated. Our results showed that the formation of complex coacervate (pHφ1) and dissolution of CMI/BSA insoluble complexes (pHφ2) appeared in pH near 4.85 and 2.00 respectively. FT-IR and Raman data confirmed the existence of electrostatic interaction and hydrogen bonding between CMI and BSA. The isothermal titration calorimetry (ITC) results suggested that the process of complex formation was spontaneous and exothermic. The complexation was dominated by enthalpy changes (∆Η < 0, ∆S < 0) at pH 4.00, while it was contributed by enthalpic and entropic changes (∆Η < 0, ∆S > 0) at pH 2.60. Irregularly shaped insoluble complexes and globular soluble nanocomplexes (about 150 nm) were observed in CMI/BSA complexes at pH 4.00 and 2.60 while using optical microscopy and atomic force microscopy, respectively. The sodium chloride suppression effect on CMI/BSA complexes was confirmed by the decrease of incipient pH for soluble complex formation (or pHc) and pHφ1 under different sodium chloride concentrations. This research presents a new functional system with the potential for delivering bioactive food ingredients.

Binding of Triton X-100 to bovine serum albumin as studied by surface tension measurements

1991 ◽  
Vol 22 (2) ◽  
pp. 129-133 ◽  
Author(s):  
Marianne Tribout ◽  
Sergio Paredes ◽  
Juan M. González-Mañas ◽  
Félix M. Goñi
Keyword(s):  
Surface Tension ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Triton X

scholarly journals Kinetics of Vibrio cholerae sialidase action on gangliosidic substrates at different supramolecular-organizational levels

1982 ◽  
Vol 203 (3) ◽  
pp. 735-742 ◽  
Author(s):  
Bruno Venerando ◽  
Benvenuto Cestaro ◽  
Amelia Fiorilli ◽  
Riccardo Ghidoni ◽  
Augusto Preti ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Vibrio Cholerae ◽  
Bovine Serum ◽  
Mixed Micelles ◽  
Molar Ratio ◽  
Free Carbohydrates ◽  
Triton X ◽  
Kinetics Of ◽  
Vibrio Cholerae Sialidase

Gd1a, Gd1b and Gt1b gangliosides were dispersed in the following membrane-mimicking systems: (a) homogeneous micelles; (b) mixed micelles with Gm1 ganglioside (which is resistant to the enzyme action), Triton X-100 or bovine serum albumin; (c) small unilamellar vesicles of egg phosphatidylcholine. The effect of dispersion on sialic acid release by Vibrio cholerae sialidase was studied. As reference substrates freely interacting with the enzyme the lipid-free carbohydrates of Gd1a and 3′-sialosyl-lactose were employed. The apparent Vmax. of the enzyme was, with all the gangliosides, dependent on the type of ganglioside dispersion. It was lowest for homogeneous micelles and mixed micelles with ganglioside Gm1, and increased about 6-fold for ganglioside/bovine serum albumin lipoprotein micelles, 15-fold for mixed-ganglioside/Triton X-100 micelles (optimal molar ratio 1:7.5) and 30-fold for phosphatidylcholine vesicles containing 2.5 mol% ganglioside (this proportion was optimal for enzyme activity on the vesicles). For ganglioside Gd1a, the activity on Triton X-100 mixed micelles and on mixed vesicles was even greater (3- and 6-fold respectively) than that displayed on Gd1a lipid-free carbohydrate. With each of the used gangliosides the apparent Km values were very similar values for homogeneous micelles and vesicular dispersions, but showed marked increases for Triton X-100 mixed micelles, approaching the values exhibited by reference oligosaccharides. Triton X-100 micelles and phosphatidylcholine vesicles did not appreciably alter the kinetics of sialidase action on 3′-sialosyl-lactose and on Gd1a lipid-free carbohydrate, indicating that the above effects are dependent on the intrinsic characteristics of the membrane-like systems containing gangliosides.

scholarly journals Evidence for cooperative binding of Triton X-100 to bovine serum albumin

FEBS Letters ◽  
1974 ◽  
Vol 42 (1) ◽  
pp. 36-41 ◽  
Author(s):  
Wayne W. Sukow ◽  
Howard E. Sandberg
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Cooperative Binding ◽  
Triton X
Sign in / Sign up

Export Citation Format

Share Document