Probing the resolution limits and tip interactions of atomic force microscopy in the study of globular proteins

Langmuir ◽  
1993 ◽  
Vol 9 (9) ◽  
pp. 2281-2288 ◽  
Author(s):  
Steven J. Eppell ◽  
Fredy R. Zypman ◽  
Roger E. Marchant
Keyword(s):  
Atomic Force Microscopy ◽  
Globular Proteins ◽  
Force Microscopy ◽  
Atomic Force ◽  
Resolution Limits

scholarly journals Atomic force microscopy and Raman scattering spectroscopy studies on heat-induced fibrous aggregates ofβ-lactoglobulin

2003 ◽  
Vol 17 (2-3) ◽  
pp. 195-202 ◽  
Author(s):  
Shinya Ikeda
Keyword(s):  
Atomic Force Microscopy ◽  
Raman Scattering ◽  
Hydrophobic Interactions ◽  
Globular Proteins ◽  
Raman Scattering Spectroscopy ◽  
Force Microscopy ◽  
Denatured Protein ◽  
Neutral Ph ◽  
Atomic Force ◽  
Protein Molecules

Nanometer-thick fibrous aggregates ofβ-lactoglobulin alone and its mixture with other globular proteins were formed by heating aqueous solutions at pH 2 with maintaining an effective level of electrostatic repulsion among denatured protein molecules. In atomic force microscopy (AFM) images, these fibrous aggregates appeared to be fairly uniform in width and height and composed of strings of globular elements. Fibrous aggregates formed inβ-lactoglobulin individual systems were only slightly thicker than the size of the nativeβ-lactoglobulin monomer, while those formed in the presence of other globular proteins were more than twice thicker, suggesting that different species of globular proteins were incorporated into each individual fibrous aggregate in the mixed systems. At neutral pH, aggregates were generally composed of ellipsoidal primary particles much larger than the size of the monomer, suggesting that aggregation proceeds in two steps at neutral pH. Molecular structural changes probed by Raman scattering spectroscopy revealed that considerable fractions ofβ-sheet structures remained to be folded during the formation of fibrous aggregates butα-helix structures were partially lost. It was also suggested that a limited extent of hydrophobic interactions among heat-denatured protein molecules is required for the fibrous aggregation.

Electrostatic Forces on the Surface of Metals as Measured by Atomic Force Microscopy

2000 ◽  
Vol 10 (1-2) ◽  
pp. 15
Author(s):  
Eugene Sprague ◽  
Julio C. Palmaz ◽  
Cristina Simon ◽  
Aaron Watson
Keyword(s):  
Atomic Force Microscopy ◽  
Electrostatic Forces ◽  
Force Microscopy ◽  
Atomic Force

In Operando Detection of the Physical Properties Change of the Interfacial Electrolyte during Li-Metal Electrode Reaction by Atomic Force Microscopy

2020 ◽  
Author(s):  
Mitsunori Kitta
Keyword(s):  
Atomic Force Microscopy ◽  
Energy Dissipation ◽  
Physical Properties ◽  
Electrode Surface ◽  
Electrode Reaction ◽  
Metal Electrode ◽  
Ion Concentration ◽  
Force Microscopy ◽  
Atomic Force ◽  
Discharge Reaction

This manuscript propose the operando detection technique of the physical properties change of electrolyte during Li-metal battery operation.The physical properties of electrolyte solution such as viscosity (η) and mass densities (ρ) highly affect the feature of electrochemical Li-metal deposition on the Li-metal electrode surface. Therefore, the operando technique for detection these properties change near the electrode surface is highly needed to investigate the true reaction of Li-metal electrode. Here, this study proved that one of the atomic force microscopy based analysis, energy dissipation analysis of cantilever during force curve motion, was really promising for the direct investigation of that. The solution drag of electrolyte, which is controlled by the physical properties, is directly concern the energy dissipation of cantilever motion. In the experiment, increasing the energy dissipation was really observed during the Li-metal dissolution (discharge) reaction, understanding as the increment of η and ρ of electrolyte via increasing of Li-ion concentration. Further, the dissipation energy change was well synchronized to the charge-discharge reaction of Li-metal electrode.This study is the first report for direct observation of the physical properties change of electrolyte on Li-metal electrode reaction, and proposed technique should be widely interesting to the basic interfacial electrochemistry, fundamental researches of solid-liquid interface, as well as the battery researches.

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