Measurement of the Interaction Forces between Proteins and Iniferter-Based Graft-Polymerized Surfaces with an Atomic Force Microscope in Aqueous Media

Langmuir ◽  
2001 ◽  
Vol 17 (4) ◽  
pp. 1080-1087 ◽  
Author(s):  
Satoru Kidoaki ◽  
Yasuhide Nakayama ◽  
Takehisa Matsuda
Keyword(s):  
Atomic Force Microscope ◽  
Aqueous Media ◽  
Interaction Forces ◽  
Atomic Force

Measuring the Interaction Forces between Protein Inclusion Bodies and an Air Bubble Using an Atomic Force Microscope

2001 ◽  
Vol 17 (5) ◽  
pp. 963-969 ◽  
Author(s):  
N.D. Wangsa-Wirawan ◽  
A. Ikai ◽  
B.K. O'Neill ◽  
A.P.J. Middelberg
Keyword(s):  
Atomic Force Microscope ◽  
Inclusion Bodies ◽  
Interaction Forces ◽  
Air Bubble ◽  
Atomic Force

Soft matter interactions at the molecular scale: interaction forces and energies between single hydrophobic model peptides

2017 ◽  
Vol 19 (6) ◽  
pp. 4216-4221 ◽  
Author(s):  
Philipp Stock ◽  
Thomas Utzig ◽  
Markus Valtiner
Keyword(s):  
Atomic Force Microscope ◽  
Soft Matter ◽  
Interaction Forces ◽  
Scale Interaction ◽  
Atomic Force ◽  
Molecular Scale ◽  
Model Peptides

The interaction between single hydrophobic molecules is quantitatively characterized by using an atomic force microscope (AFM).

Measuring forces between biological macromolecules with the Atomic Force Microscope: characterization and applications

1995 ◽  
Vol 53 ◽  
pp. 718-719
Author(s):  
Gil U Lee ◽  
Linda Chrisey ◽  
Richard J. Colton
Keyword(s):  
Atomic Force Microscope ◽  
Molecular Interactions ◽  
Molecular Interaction ◽  
Variable Stiffness ◽  
Force Transducer ◽  
Resonance Spectroscopy ◽  
Biological Macromolecules ◽  
Receptor System ◽  
Interaction Forces ◽  
Atomic Force

Structure and function in biological macromolecular systems such as proteins and polynucleotides are based on intermolecular interactions that are short ranged and chemically specific. Our knowledge of these molecular interactions results from indirect physical and thermodynamic measurements such as x-ray crystallography, light scattering and nuclear magnetic resonance spectroscopy. Direct measurement of molecular interaction forces requires that the state of a system be monitored with near atomic resolution while an independent force is applied to the system of 10−12 to 10−9 Newton magnitude. The atomic force microscope (AFM) has recently been applied to the study of single molecular interactions. The microfabricated cantilever of the AFM, a force transducer of small yet variable stiffness and high resonance frequency, produces a transducer of 10−15 N/Hz1/2 force sensitivities and 0.01 nm position accuracy.This presentation describes the AFM measurement of the molecular interaction forces in the model ligand-receptor system streptavidin-biotin and between complementary strands of DNA.

Data analysis of interaction forces measured with the atomic force microscope

2000 ◽  
Vol 82 (1-4) ◽  
pp. 85-95 ◽  
Author(s):  
W. Baumgartner ◽  
P. Hinterdorfer ◽  
H. Schindler
Keyword(s):  
Data Analysis ◽  
Atomic Force Microscope ◽  
Interaction Forces ◽  
Atomic Force

Dynamic analysis of AFM by applying Timoshenko beam theory in tapping mode and considering the impact of interaction forces in a liquid environment

2014 ◽  
Vol 92 (6) ◽  
pp. 472-483 ◽  
Author(s):  
M. Damircheli ◽  
M.H. Korayem
Keyword(s):  
Dynamic Response ◽  
Atomic Force Microscope ◽  
Timoshenko Beam ◽  
Ambient Air ◽  
Beam Model ◽  
Timoshenko Beam Model ◽  
Interaction Forces ◽  
Liquid Environment ◽  
Atomic Force ◽  
Simulation Results

In an atomic force microscope (AFM), the cantilever vibrates by excitation at a frequency near the fundamental frequency, and the changes in vibration parameters, which result from the nonlinear forces of interaction between sample and cantilever tip, can be used as a tool to reveal the properties of the sample. To properly describe the images acquired by the AFM and to approximate the properties of the investigated sample, it is essential to use analytical and numerical models that can accurately simulate the dynamics of the cantilever and sample. For short beams, the Timoshenko model seems to be very accurate. Considering the fact that short beams (cantilevers) have many applications including the imaging of biological samples in liquid environments, the use of this theory seems to be necessary. In this paper, by employing the Timoshenko beam model, the effect of rotational inertia and shear deformation has been taken into consideration. The interaction forces between sample and cantilever in liquid, ambient air, and vacuum environments are quite different in terms of magnitude and formulation, and they play a significant role in the system’s dynamic response. These forces include hydrodynamic forces, electrostatic double layer force, etc. Using an accurate model for the interaction forces will improve the simulation results significantly. In this paper, the frequency response of the atomic force microscope has been investigated by applying the Timoshenko beam model and considering the forces of interaction between sample and tip in the air and liquid environments. The results indicate that the resonant frequency changes and cantilever vibration amplitude diminishes in a liquid environment compared to the air environment. The simulation results have good agreement with the experimental ones. The frequency responses for the attractive and repulsive regions in the two environments are compared and it is demonstrated that the dynamic response is highly dependent on the hydrodynamic and interaction forces in the liquid medium.

scholarly journals Mapping interaction forces with the atomic force microscope

1994 ◽  
Vol 66 (6) ◽  
pp. 2159-2165 ◽  
Author(s):  
M. Radmacher ◽  
J.P. Cleveland ◽  
M. Fritz ◽  
H.G. Hansma ◽  
P.K. Hansma
Keyword(s):  
Atomic Force Microscope ◽  
Interaction Forces ◽  
Atomic Force
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