Lysis of Human Red Blood Cells. 4. Comparison of in Vitro and in Vivo Hemolysis Data

1997 ◽  
Vol 86 (11) ◽  
pp. 1215-1217 ◽  
Author(s):  
Joseph F. Krzyzaniak ◽  
Fernando A. Alvarez Núñez ◽  
Dawn M. Raymond ◽  
Samuel H. Yalkowsky
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Red Blood Cells

Decarboxylation of Radioactive DOPA by Erythrocytes in Schizophrenia

1971 ◽  
Vol 118 (545) ◽  
pp. 465-466 ◽  
Author(s):  
Ngo Tran ◽  
Marcel Laplante ◽  
Etienne Lebel
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Ionization Chamber ◽  
Present Experiment ◽  
Continuous Measurement ◽  
Human Tissues ◽  
Chamber Method ◽  
Human Red Blood Cells

The decarboxylation of 3, 4-dihydroxyphenyl-alanine (Dopa) to dopamine has been shown previously in animal and human tissues in both in vitro and in vivo studies (Sourkes, 1966; Vogel et al., 1970). However, very little information is available as to whether or not the decarboxylation of Dopa occurs in human red blood cells (RBC). In the present experiment we demonstrated this change in RBC from normals and from schizophrenics. An ionization chamber method was used for an instantaneous and continuous measurement of 14CO2 production from DL-dopa-carboxyl-14C by RBC in vitro.

In vivo and in vitro penetration of vitamins into human red blood cells

1971 ◽  
Vol 24 (8) ◽  
pp. 924-929 ◽  
Author(s):  
Michael F. Sorrell ◽  
Oscar Frank ◽  
Hermes Aquino ◽  
Allan D. Thomson ◽  
Herman Baker
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Red Blood Cells

Alteration of human red blood cells stored in plastic packs. A case report with in vivo and in vitro studies

Transfusion ◽  
1982 ◽  
Vol 22 (2) ◽  
pp. 154-157 ◽  
Author(s):  
JT Callahan ◽  
MF Collecutt ◽  
JR Lightbody ◽  
BS Faragher
Keyword(s):  
Case Report ◽  
Red Blood Cells ◽  
Blood Cells ◽  
In Vitro Studies ◽  
Human Red Blood Cells

scholarly journals Spectrin and Other Membrane-Skeletal Components in Human Red Blood Cells of Different Age

2017 ◽  
Vol 42 (3) ◽  
pp. 1139-1152 ◽  
Author(s):  
Annarita Ciana ◽  
Cesare Achilli ◽  
Giampaolo Minetti
Keyword(s):  
Red Blood Cells ◽  
Surface Area ◽  
Blood Cells ◽  
Ubiquitin Proteasome System ◽  
Membrane Skeleton ◽  
Experimental Conditions ◽  
Human Red Blood Cells ◽  
Cell Age

Background: Old human red blood cells (RBCs) have a reduced surface area with respect to young RBCs. If this decrease occurred through the release of vesicles similar to the spectrin-free vesicles that are shed in vitro under different experimental conditions or during storage, there would be no decrease of membrane-skeleton, but only of lipid bilayer surface area, during RBC ageing in vivo. However, we observed a decrease in spectrin and other membrane-skeletal proteins in old RBCs. Because RBCs contain components of the ubiquitin-proteasome system and other hydrolytic systems for protein degradation, we asked whether increased membrane-skeleton fragments could be detected in older RBCs. Methods: Four different anti-spectrin antibodies and an antibody anti-ubiquitin conjugates were used to analyse, by Western blotting, fragments of spectrin and other proteins in RBCs of different age separated in density gradients and characterized for their protein 4.1a/4.1b ratio as a cell age parameter. Results: spectrin fragments do exist in RBCs of all ages, they represent a minute fraction of all spectrin, are membrane-bound and not cytoplasmic and do not increase with cell age. Besides spectrin, other membrane-skeletal components decrease with cell age. Conclusion: Observed results challenge the commonly accepted view that decrease in cell membrane throughout RBC life in vivo occurs via the release of spectrin-free vesicles.

In vitro and in vivo Binding of Epirubicin to Red Blood Cells and Human Plasma Proteins

1994 ◽  
Vol 49 (7-8) ◽  
pp. 483-488 ◽  
Author(s):  
Suzan Bandak ◽  
Martin Czejka ◽  
Johann Schüller
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Plasma Proteins ◽  
In Vivo Binding ◽  
Human Red Blood Cells ◽  
Encapsulation Rate ◽  
Vitro Binding ◽  
In Vitro Interaction

In this study, the in vitro interaction of epirubicin (EPR), a cytostatic antibiotic, with plasma proteins (PP), namely α-HSA, γ-HSG, α+β-HSG and with isolated human red blood cells (RBCs) was investigated and further correlated with the in vivo pharmacokinetics and binding of EPR and two of its metabolites, 13-dihydroepirubicin and 7-deoxydoxorubicinone to RBCs. The in vitro encapsulation rate in isolated erythrocytes amounts to 52.9 ± 2.8% and remains constant within the range of studied concentrations (2.5-20 μg/ml). EPR was found to bind differently to the various PP in vitro. Binding to α-HSA amounted up to 51.0 ± 7.10%, to α + β-HSG 79.45 ± 2.7%, to γ-HSG 57.1 ± 2.8%. The in vivo-binding rate of EPR, dihydroepirubicin and deoxydoxorubicinone to RBCs after 5 min of injection was 32 ± 6.96%, 11.6 ± 3.1% and 10.05 ± 3.5% respectively, their availability in serum was 42.6 ± 11.8%, 2.4 ± 0.4% and 1.2 ± 0.67% respectively

Ultrastructural observations on the in vitro cytoadherence of Plasmodium falciparum infected red blood cells

1990 ◽  
Vol 48 (3) ◽  
pp. 914-915
Author(s):  
D.J.P. Ferguson ◽  
A.R. Berendt ◽  
J. Tansey ◽  
K. Marsh ◽  
C.I. Newbold
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Umbilical Vein ◽  
Cell Types ◽  
Amelanotic Melanoma ◽  
Cytoplasmic Process ◽  
Umbilical Vein Endothelial Cells

In human malaria, the most serious clinical manifestation is cerebral malaria (CM) due to infection with Plasmodium falciparum. The pathology of CM is thought to relate to the fact that red blood cells containing mature forms of the parasite (PRBC) cytoadhere or sequester to post capillary venules of various tissues including the brain. This in vivo phenomenon has been studied in vitro by examining the cytoadherence of PRBCs to various cell types and purified proteins. To date, three Ijiost receptor molecules have been identified; CD36, ICAM-1 and thrombospondin. The specific changes in the PRBC membrane which mediate cytoadherence are less well understood, but they include the sub-membranous deposition of electron-dense material resulting in surface deformations called knobs. Knobs were thought to be essential for cytoadherence, lput recent work has shown that certain knob-negative (K-) lines can cytoadhere. In the present study, we have used electron microscopy to re-examine the interactions between K+ PRBCs and both C32 amelanotic melanoma cells and human umbilical vein endothelial cells (HUVEC).We confirm previous data demonstrating that C32 cells possess numerous microvilli which adhere to the PRBC, mainly via the knobs (Fig. 1). In contrast, the HUVEC were relatively smooth and the PRBCs appeared partially flattened onto the cell surface (Fig. 2). Furthermore, many of the PRBCs exhibited an invagination of the limiting membrane in the attachment zone, often containing a cytoplasmic process from the endothelial cell (Fig. 2).

scholarly journals Multimodal Diagnostics of Microrheologic Alterations in Blood of Coronary Heart Disease and Diabetic Patients

Diagnostics ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 76
Author(s):  
Anastasia Maslianitsyna ◽  
Petr Ermolinskiy ◽  
Andrei Lugovtsov ◽  
Alexandra Pigurenko ◽  
Maria Sasonko ◽  
...  
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Optical Methods ◽  
Diabetic Patients ◽  
Aggregation Index ◽  
Significant Difference

Coronary heart disease (CHD) has serious implications for human health and needs to be diagnosed as early as possible. In this article in vivo and in vitro optical methods are used to study blood properties related to the aggregation of red blood cells in patients with CHD and comorbidities such as type 2 diabetes mellitus (T2DM). The results show not only a significant difference of the aggregation in patients compared to healthy people, but also a correspondence between in vivo and in vitro parameters. Red blood cells aggregate in CHD patients faster and more numerously; in particular the aggregation index increases by 20 ± 7%. The presence of T2DM also significantly elevates aggregation in CHD patients. This work demonstrates multimodal diagnostics and monitoring of patients with socially significant pathologies.

In vitro effects of low level yellow laser irradiation on human red blood cells

2016 ◽  
Author(s):  
Mustafa S. Al Musawi ◽  
M.S. Jaafar ◽  
B.T. Al-Gailani ◽  
Naser M. Ahmed ◽  
Fatanah M. Suhaimi
Keyword(s):  
Red Blood Cells ◽  
Laser Irradiation ◽  
Blood Cells ◽  
Low Level ◽  
Human Red Blood Cells ◽  
Yellow Laser ◽  
In Vitro Effects

Clock gene-independent daily regulation of haemoglobin oxidation in red blood cells

2021 ◽  
Author(s):  
Andrew D. Beale ◽  
Priya Crosby ◽  
Utham K. Valekunja ◽  
Rachel S. Edgar ◽  
Johanna E. Chesham ◽  
...  
Keyword(s):  
Circadian Rhythms ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Human Subjects ◽  
Developmental Regulation ◽  
Physiological Role ◽  
Cell Types ◽  
The Body

AbstractCellular circadian rhythms confer daily temporal organisation upon behaviour and physiology that is fundamental to human health and disease. Rhythms are present in red blood cells (RBCs), the most abundant cell type in the body. Being naturally anucleate, RBC circadian rhythms share key elements of post-translational, but not transcriptional, regulation with other cell types. The physiological function and developmental regulation of RBC circadian rhythms is poorly understood, however, partly due to the small number of appropriate techniques available. Here, we extend the RBC circadian toolkit with a novel biochemical assay for haemoglobin oxidation status, termed “Bloody Blotting”. Our approach relies on a redox-sensitive covalent haem-haemoglobin linkage that forms during cell lysis. Formation of this linkage exhibits daily rhythms in vitro, which are unaffected by mutations that affect the timing of circadian rhythms in nucleated cells. In vivo, haemoglobin oxidation rhythms demonstrate daily variation in the oxygen-carrying and nitrite reductase capacity of the blood, and are seen in human subjects under controlled laboratory conditions as well as in freely-behaving humans. These results extend our molecular understanding of RBC circadian rhythms and suggest they serve an important physiological role in gas transport.

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