The Role of P-Glycoprotein and Canalicular Multispecific Organic Anion Transporter in the Hepatobiliary Excretion of Drugs

1998 ◽  
Vol 87 (9) ◽  
pp. 1025-1040 ◽  
Author(s):  
Hiroyuki Kusuhara ◽  
Hiroshi Suzuki ◽  
Yuichi Sugiyama
Keyword(s):  
Organic Anion ◽  
Organic Anion Transporter ◽  
P Glycoprotein ◽  
Anion Transporter

scholarly journals SLCO4A1-AS1 Facilitates the Malignant Phenotype via miR-149-5p/STAT3 Axis in Gastric Cancer Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Qing Li ◽  
Dachuan Zhang ◽  
Hui Wang ◽  
Jun Xie ◽  
Lei Peng ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Organic Anion ◽  
Organic Anion Transporter ◽  
Gastric Cancer Cells ◽  
Anion Transporter ◽  
Transporter Family ◽  
Cancer Tissues ◽  
Development Of Gastric Cancer

Solute carrier organic anion transporter family member 4A1 (SLCO4A1-AS1), a newly discovered lncRNA, may exert effects in tumors. Since its role in gastric cancer remains obscure, we sought to explore the mechanism of SLCO4A1-AS1 in gastric cancer. The relationship among SLCO4A1-AS1, miR-149-5p, and STAT3 was detected by bioinformatics, dual luciferase analysis, and Pearson’s test, and the expressions of these genes were determined by quantitative real-time PCR and Western blot. Moreover, CCK-8, flow cytometry, wound healing assay, and Transwell analysis were performed to verify the function of SLCO4A1-AS1 in gastric cancer. Rescue experiments were used to detect the role of miR-149-5p. The expressions of SLCO4A1-AS1 and STAT3 were increased, while the expression of miR-149-5p was suppressed in gastric cancer tissues and cell lines. In addition, STAT3 expression was negatively correlated with miR-149-5p expression but was positively correlated with SLCO4A1-AS1 expression. Overexpression of SLCO4A1-AS1 promoted cell viability, migration, invasion, and STAT3 expression but suppressed apoptosis, while knockdown of SLCO4A1-AS1 had the opposite effect. SLCO4A1-AS1 bound to miR-149-5p and targeted STAT3. Moreover, miR-149-5p mimic inhibited the malignant development of gastric cancer cells and obviously reversed the function of SLCO4A1-AS1 overexpression. Our research reveals that abnormally increased SLCO4A1-AS1 expression may be an important molecular mechanism in the development of gastric cancer.

scholarly journals Role of human organic anion transporter 4 in the transport of ochratoxin A

2002 ◽  
Vol 1590 (1-3) ◽  
pp. 64-75 ◽  
Author(s):  
Ellappan Babu ◽  
Michio Takeda ◽  
Shinichi Narikawa ◽  
Yukari Kobayashi ◽  
Atsushi Enomoto ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Organic Anion ◽  
Organic Anion Transporter ◽  
Anion Transporter

scholarly journals Evaluation of the endothelin receptor antagonists ambrisentan, darusentan, bosentan, and sitaxsentan as substrates and inhibitors of hepatobiliary transporters in sandwich-cultured human hepatocytesThis article is one of a selection of papers published in the two-part special issue entitled 20 Years of Endothelin Research.

2010 ◽  
Vol 88 (6) ◽  
pp. 682-691 ◽  
Author(s):  
J. Craig Hartman ◽  
Kenneth Brouwer ◽  
Arun Mandagere ◽  
Lawrence Melvin ◽  
Richard Gorczynski
Keyword(s):  
Organic Anion ◽  
Hepatic Uptake ◽  
Organic Anion Transporter ◽  
Endothelin Receptor Antagonists ◽  
Endothelin Receptor ◽  
Transport Activity ◽  
Receptor Antagonists ◽  
P Glycoprotein ◽  
Anion Transporter ◽  
Hepatic Transporters

To evaluate potential mechanisms of clinical hepatotoxicity, 4 endothelin receptor antagonists (ERAs) were examined for substrate activity and inhibition of hepatic uptake and efflux transporters in sandwich-cultured human hepatocytes. The 4 transporters studied were sodium-dependent taurocholate cotransporter (NTCP), organic anion transporter (OATP), bile salt export pump (BSEP), and multidrug resistance-associated protein 2 (MRP2). ERA transporter inhibition was examined using the substrates taurocholate (for NTCP and BSEP), [3H]estradiol-17β-d-glucuronide (for OATP), and [2-d-penicillamine, 5-d-penicillamine]enkephalin (for MRP2). ERA substrate activity was evaluated using probe inhibitors ritonavir (OATP and BSEP), bromosulfalein (OATP), erythromycin (P-glycoprotein), probenecid (MRP2 and OATP), and cyclosporin (NTCP). ERAs were tested at 2, 20, and 100 µmol·L–1 for inhibition and at 2 µmol·L–1 as substrates. OATP, NTCP, or BSEP transport activity was not reduced by ambrisentan or darusentan. Bosentan and sitaxsentan attenuated NTCP transport at higher concentrations. Only sitaxsentan decreased OATP transport (52%), and only bosentan reduced BSEP transport (78%). MRP2 transport activity was unaltered. OATP inhibitors decreased influx of all ERAs. Darusentan influx was least affected (84%–100% of control), whereas bosentan was most affected (32%–58% of control). NTCP did not contribute to influx of ERAs. Only bosentan and darusentan were shown as substrates for both BSEP and P-glycoprotein efflux. All ERAs tested were substrates for at least one hepatic transporter. Bosentan and sitaxsentan, but not ambrisentan and darusentan, inhibited human hepatic transporters, which provides a potential mechanism for the increased hepatotoxicity observed for these agents in the clinical setting.

Role of organic anion transporter 3 (Oat3) in the renal transport of glutathione (GSH)

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Lawrence H. Lash ◽  
David A. Putt ◽  
Feng Xu ◽  
Larry H. Matherly
Keyword(s):  
Organic Anion ◽  
Organic Anion Transporter ◽  
Renal Transport ◽  
Anion Transporter ◽  
Organic Anion Transporter 3

Role of Hepatic Organic Anion Transporter 2 in the Pharmacokinetics ofR- andS-Warfarin: In Vitro Studies and Mechanistic Evaluation

2018 ◽  
Vol 15 (3) ◽  
pp. 1284-1295 ◽  
Author(s):  
Yi-an Bi ◽  
Jian Lin ◽  
Sumathy Mathialagan ◽  
Laurie Tylaska ◽  
Ernesto Callegari ◽  
...  
Keyword(s):  
Organic Anion ◽  
In Vitro Studies ◽  
Organic Anion Transporter ◽  
Anion Transporter

scholarly journals Major role of organic anion transporter 3 in the transport of indoxyl sulfate in the kidney

2002 ◽  
Vol 61 (5) ◽  
pp. 1760-1768 ◽  
Author(s):  
Tsuneo Deguchi ◽  
Sumio Ohtsuki ◽  
Masaki Otagiri ◽  
Hitomi Takanaga ◽  
Hiroshi Asaba ◽  
...  
Keyword(s):  
Organic Anion ◽  
Organic Anion Transporter ◽  
Indoxyl Sulfate ◽  
Anion Transporter ◽  
Organic Anion Transporter 3
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