A Sensitive High-Pressure Liquid Chromatography/Particle Beam/Mass Spectrometry Assay for the Determination of all-trans-Retinoic Acid and 13-cis-Retinoic Acid in Human Plasma

1996 ◽  
Vol 85 (3) ◽  
pp. 287-290 ◽  
Author(s):  
Paul A. Lehman ◽  
Thomas J. Franz
Keyword(s):  
Mass Spectrometry ◽  
High Pressure ◽  
Liquid Chromatography ◽  
Retinoic Acid ◽  
Human Plasma ◽  
Particle Beam ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid ◽  
Mass Spectrometry Assay

scholarly journals Simultaneous Determination of all-trans-Retinoic Acid, β-Carotene, and Vitamin A in Galenic Preparations by Liquid Chromatography

2001 ◽  
Vol 84 (2) ◽  
pp. 354-360
Author(s):  
Vincenzo Pucci ◽  
Francesca Bugamelli ◽  
Roberto Mandrioli ◽  
Maria A Raggi
Keyword(s):  
Liquid Chromatography ◽  
Retinoic Acid ◽  
Vitamin A ◽  
Degradation Products ◽  
Extraction Procedure ◽  
Uv Detector ◽  
Trans Retinoic Acid ◽  
All Trans Retinoic Acid ◽  
Β Carotene

Abstract The concentrations of vitamin A, β-carotene, and all-trans-retinoic acid in oral preparations were determined in a single analysis by a method based on isocratic, reversed-phase liquid chromatography (LC). The LC system consisted of a C18 column, a mobile phase of acetonitrile, dichloromethane, methanol, and water and a UV detector set at 330 nm. The linearity ranges were 25–250 ng/mL for trans-retinoic acid and vitamin A, and 100–1000 ng/mL for β-carotene. This LC method for the determination of retinoids is simple, precise, and accurate. No extraction procedure is required before the chromatographic analysis; only a suitable dilution is necessary. The method proved to be reliable, fast, and economical. Furthermore, this method is indicative of stability, because it allows for the determination of degradation products such as 13-cis-retinoic acid.

Determination of deuterium-labeled phenylalanine and tyrosine in human plasma with high pressure liquid chromatography and mass spectrometry

1976 ◽  
Vol 73 (3) ◽  
pp. 431-438 ◽  
Author(s):  
Friedrich K. Trefz ◽  
Dennis J. Byrd ◽  
Milan E. Blaskovics ◽  
Walter Kochen ◽  
Peter Lutz
Keyword(s):  
Mass Spectrometry ◽  
High Pressure ◽  
Liquid Chromatography ◽  
Human Plasma ◽  
High Pressure Liquid Chromatography
Sign in / Sign up

Export Citation Format

Share Document