Experimental Study on Conformational Changes of Lysozyme in Solution Induced by Pulsed Electric Field and Thermal Stresses

2010 ◽  
Vol 114 (1) ◽  
pp. 503-510 ◽  
Author(s):  
Wei Zhao ◽  
Ruijin Yang
Keyword(s):  
Experimental Study ◽  
Electric Field ◽  
Conformational Changes ◽  
Thermal Stresses ◽  
Pulsed Electric Field

scholarly journals Receptor Binding Domain (RBD) Structural Susceptibility in the SARS-CoV-2 Virus Spike Protein Exposed to a Pulsed Electric Field

2021 ◽  
Vol 8 (2) ◽  
pp. 177-182
Author(s):  
D. Osorio-González ◽  
V. J. Muñiz-Orozco ◽  
C. P. González ◽  
M. Fuentes-Acosta ◽  
J. Mulia-Rodríguez ◽  
...  
Keyword(s):  
Electric Field ◽  
Receptor Binding ◽  
Conformational Changes ◽  
In Silico Analysis ◽  
Pulsed Electric Field ◽  
Binding Domain ◽  
Chemical Components ◽  
Receptor Binding Domain ◽  
Viral Inhibition ◽  
Starting Point

SARS-CoV-2 is responsible for causing the Coronavirus disease 2019 (COVID-19) pandemic, which has so far infected more than thirty million people and caused almost a million deaths. For this reason, it has been a priority to stop the transmission of the outbreak through preventive measures, such as surface disinfection, and to establish bases for the design of an effective disinfection technique without chemical components. In this study, we performed in silico analysis to identify the conformational alterations of the SARS-CoV-2 Spike Receptor Binding Domain (RBD) caused by the effect of a pulsed electric field at two different intensities. We found that both stimuli, especially the one with the highest angular frequency and amplitude, modified the electrical charge distribution in the RBD surface and the number of hydrogen bonds. Moreover, the secondary structure was significantly affected, with a decrease of the structured regions, particularly the regions with residues involved in recognizing and interacting with the receptor ACE2. Since many regions suffered conformational changes, we calculated RMSF and ΔRMSF to identify the regions and residues with larger fluctuations and higher flexibility. We found that regions conformed by 353-372, 453-464, and 470-490 amino acid residues fluctuate the most, where the first is considered a therapeutic target, and the last has alreadybeen characterized for its flexibility. Our results indicate that a pulsed electric field can cause loss of stability in the Spike-RBD, and we were able to identify the vulnerable sites to be used as a starting point for the development of viral inhibition or inactivation mechanisms.

In vivo experimental study of nanosecond pulsed electric field effects on solid tumors

2013 ◽  
Vol 20 (4) ◽  
pp. 1266-1272 ◽  
Author(s):  
Masataka Nagahama ◽  
Naoyuki Shimomura ◽  
Akito Nakagawa ◽  
Kenji Teranishi ◽  
Yoshihiro Uto ◽  
...  
Keyword(s):  
Experimental Study ◽  
Electric Field ◽  
Solid Tumors ◽  
Pulsed Electric Field ◽  
Electric Field Effects ◽  
Field Effects ◽  
Nanosecond Pulsed Electric Field

scholarly journals Effects of an Electric Field on the Conformational Transition of the Protein: Pulsed and Oscillating Electric Fields with Different Frequencies

Polymers ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 123
Author(s):  
Qun Zhang ◽  
Dongqing Shao ◽  
Peng Xu ◽  
Zhouting Jiang
Keyword(s):  
Dipole Moment ◽  
Secondary Structure ◽  
Electric Field ◽  
Root Mean Square ◽  
Structure Analysis ◽  
Electric Fields ◽  
Conformational Changes ◽  
Pulsed Electric Field ◽  
Mean Square ◽  
Secondary Structure Analysis

The effect of pulsed and oscillating electric fields with different frequencies on the conformational properties of all-α proteins was investigated by molecular dynamics simulations. The root mean square deviation, the root mean square fluctuation, the dipole moment distribution, and the secondary structure analysis were used to assess the protein samples’ structural characteristics. In the simulation, we found that the higher frequency of the electric field influences the rapid response to the secondary structural transitions. However, the conformational changes measured by RMSD are diminished by applying the electrical field with a higher frequency. During the dipole moment analysis, we found that the magnitude and frequency of the dipole moment was directly related to the strength and frequency of the external electric field. In terms of the type of electric fields, we found that the average values of RMSD and RMSF of whole molecular protein are larger when the protein is exposed in the pulsed electric field. Concerning the typical sample 1BBL, the secondary structure analysis showed that two alpha-helix segments both transit to turns or random coils almost simultaneously when it is exposed in a pulsed electric field. Meanwhile, two segments present the different characteristic times when the transition occurs in the condition of an oscillating electric field. This study also demonstrated that the protein with fewer charged residues or more residues in forming α-helical structures display the higher conformational stability. These conclusions, achieved using MD simulations, provide a theoretical understanding of the effect of the frequency and expression form of external electric fields on the conformational changes of the all-α proteins with charged residues and the guidance for anticipative applications.

Influence of Pulse Width on Polyphenol Extraction from Agricultural Products by Pulsed Electric Field

2013 ◽  
Vol 133 (2) ◽  
pp. 32-37 ◽  
Author(s):  
Akira Nakagawa ◽  
Hitoshi Hatayama ◽  
Koichi Takaki ◽  
Shoji Koide ◽  
Yukio Kawamura
Keyword(s):  
Electric Field ◽  
Pulse Width ◽  
Pulsed Electric Field ◽  
Agricultural Products ◽  
Polyphenol Extraction

Development of PEF extraction technology by Maguin

2015 ◽  
pp. 758-760
Author(s):  
Romain Delecourt ◽  
Loïc Marsal
Keyword(s):  
Sugar Beet ◽  
Electric Field ◽  
Pulsed Electric Field ◽  
Extraction Technology ◽  
Application System ◽  
Wide Range ◽  
Type Machine

Maguin (France) is still active in the application of pulsed electric field (PEF) technology. After having carried out successful tests on a 10 t/h pilot screw-type machine on sugar beet cossettes, a new application system based on a roller technology has been developed. This technology allows a wide range of application due to its flexibility with flowrates and materials. A variety of process schemes are proposed to ensure the best performance of the PEF technology.

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