scholarly journals Ionic Surfactant Aggregates in Saline Solutions: Sodium Dodecyl Sulfate (SDS) in the Presence of Excess Sodium Chloride (NaCl) or Calcium Chloride (CaCl2)

2009 ◽  
Vol 113 (17) ◽  
pp. 5863-5870 ◽  
Author(s):  
Maria Sammalkorpi ◽  
Mikko Karttunen ◽  
Mikko Haataja
Keyword(s):  
Sodium Chloride ◽  
Sodium Dodecyl Sulfate ◽  
Calcium Chloride ◽  
Sodium Dodecyl ◽  
Ionic Surfactant ◽  
Surfactant Aggregates ◽  
Dodecyl Sulfate ◽  
Excess Sodium ◽  
Saline Solutions

scholarly journals Analysis of Some Biogenic Amines by Micellar Liquid Chromatography

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Irena Malinowska ◽  
Katarzyna E. Stępnik
Keyword(s):  
Liquid Chromatography ◽  
Sodium Dodecyl Sulfate ◽  
Biogenic Amines ◽  
Physicochemical Parameters ◽  
Mobile Phase ◽  
Sodium Dodecyl ◽  
Micellar Liquid Chromatography ◽  
Ionic Surfactant ◽  
Mobile Phases ◽  
Dodecyl Sulfate

Micellar liquid chromatography (MLC) with the use of high performance liquid chromatography (HPLC) was used to determine some physicochemical parameters of six biogenic amines: adrenaline, dopamine, octopamine, histamine, 2-phenylethylamine, and tyramine. In this paper, an influence of surfactant’s concentration and pH of the micellar mobile phase on the retention of the tested substances was examined. To determine the influence of surfactant’s concentration on the retention of the tested amines, buffered solutions (at pH 7.4) of ionic surfactant—sodium dodecyl sulfate SDS (at different concentrations) with acetonitrile as an organic modifier (0.8/0.2 v/v) were used as the micellar mobile phases. To determine the influence of pH of the micellar mobile phase on the retention, mobile phases contained buffered solutions (at different pH values) of sodium dodecyl sulfate SDS (at 0.1 M) with acetonitrile (0.8/0.2 v/v). The inverse of value of retention factor () versus concentration of micelles () relationships were examined. Other physicochemical parameters of solutes such as an association constant analyte—micelle ()—and partition coefficient of analyte between stationary phase and water (hydrophobicity descriptor) () were determined by the use of Foley’s equation.

Hepatic and lipoprotein lipases selectively assayed in postheparin plasma

1993 ◽  
Vol 39 (2) ◽  
pp. 218-223 ◽  
Author(s):  
A D Henderson ◽  
W Richmond ◽  
R S Elkeles
Keyword(s):  
Sodium Chloride ◽  
Sodium Dodecyl Sulfate ◽  
Lipoprotein Lipase ◽  
Sex Difference ◽  
Hepatic Lipase ◽  
Sodium Dodecyl ◽  
Plasma Samples ◽  
Dodecyl Sulfate ◽  
Normal Adults

Abstract Sensitive, reliable procedures are reported for the selective assay of lipoprotein lipase (LPL) and hepatic lipase (HL) in postheparin plasma samples. LPL is inhibited in the HL assay by inclusion of 0.76 mol/L sodium chloride in the substrate. In the LPL assay, specificity is attained by pretreating the sample with sodium dodecyl sulfate, which selectively denatures HL. This LPL method was validated by direct comparison with a procedure in which HL is inactivated by an antiserum to human HL. We used the described assays to quantify LPL and HL activity in 32 normal adults, demonstrating a clear sex difference for both enzymes. On average, the men displayed higher HL and lower LPL activities than did the women.

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