15-Crown-5 Functionalized Au Nanoparticles Synthesized via Single Molecule Exchange on Silica Nanoparticles: Its Application to Probe 15-Crown-5/K+/15-Crown-5 “Sandwiches” as Linking Mechanisms

2009 ◽  
Vol 113 (5) ◽  
pp. 1686-1693 ◽  
Author(s):  
Mei-Lin Ho ◽  
Jia-Ming Hsieh ◽  
Chih-Wei Lai ◽  
Hsin-Chieh Peng ◽  
Chia-Cheng Kang ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Single Molecule ◽  
Au Nanoparticles

SERS-BASED AQUEOUS IMMUNOASSAY REALIZED WITH SILICA NANOPARTICLES

2011 ◽  
Vol 20 (01) ◽  
pp. 63-73
Author(s):  
C. Y. SONG ◽  
Z. Y. WANG ◽  
J. YANG ◽  
R. H. ZHANG ◽  
H. WU ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Gold Nanoparticles ◽  
Detection Limit ◽  
Silica Nanoparticles ◽  
Hot Spots ◽  
Au Nanoparticles

A simple, sensitive SERS-based immunoassay realized in aqueous solution is demonstrated with a sandwich immune protocol. In such an immunoassay, antibodies-immobilized silica nanoparticles served as the immune substrate while 4MBA-labeled immuno-Au nanoparticles are used as the immune sensors. According to the TEM images, it is clear that the immune gold nanoparticles are embedded onto the surfaces of the silica nanoparticles specifically after the immunoreaction. As a result, the aggregations of gold nanoparticles have been formed with SERS-active "hot spots" on the dimers or multimers. The SERS results confirm that the method proposed in this paper is an effective way for SERS-based aqueous immunoassay and that the detection limit is as low as 0.1 ng/mL.

scholarly journals “Blinking” silica nanoparticles for optical super resolution imaging of cancer cells

RSC Advances ◽  
2017 ◽  
Vol 7 (77) ◽  
pp. 48738-48744 ◽  
Author(s):  
Jia Li ◽  
Shenfei Zong ◽  
Zhuyuan Wang ◽  
Yiping Cui
Keyword(s):  
Silica Nanoparticles ◽  
Cancer Cells ◽  
Single Molecule ◽  
Super Resolution ◽  
Resolution Imaging

A strategy to fabricate blinking silica nanoparticles is presented, which can be used in single molecule localization imaging.

A Facile Method to Synthesis Hollow Silica Ellipsoids and Their Derivatives

2012 ◽  
Vol 1453 ◽  
Author(s):  
Henan Zhang ◽  
Daniel L. Akins
Keyword(s):  
Silica Nanoparticles ◽  
Chemical Etching ◽  
Au Nanoparticles ◽  
Silica Surface ◽  
Hollow Silica ◽  
Synthesis Conditions ◽  
Wet Chemical ◽  
Wet Chemical Etching

ABSTRACTWe have utilized wet-chemical etching of ellipsoidal silica nanoparticles to form silica nanoshells of a range of elliptical morphologies. The thicknesses of the silica ellipsoidal nanoshells are controlled through variation of synthesis conditions. A mechanism has been proposed to explain how the nanoshells are formed, and we have demonstrated that the porosity of the silica ellipsoid plays a role in the dissolution-regrowth process. We have also, via appropriate functionalization of the silica surface, coated the ellipsoidal nanoshells with Au nanoparticles.

scholarly journals Challenges and Contradictions of Metal Nano-Particle Applications for Radio-Sensitivity Enhancement in Cancer Therapy

2019 ◽  
Vol 20 (3) ◽  
pp. 588 ◽  
Author(s):  
Eva Pagáčová ◽  
Lenka Štefančíková ◽  
Franz Schmidt-Kaler ◽  
Georg Hildenbrand ◽  
Tomáš Vičar ◽  
...  
Keyword(s):  
Single Molecule ◽  
Metallic Nanoparticles ◽  
Au Nanoparticles ◽  
Cell Damage ◽  
Super Resolution ◽  
Pt Nanoparticles ◽  
Cell Types ◽  
X Rays ◽  
Practical Applications ◽  
Radiation Induced

From the very beginnings of radiotherapy, a crucial question persists with how to target the radiation effectiveness into the tumor while preserving surrounding tissues as undamaged as possible. One promising approach is to selectively pre-sensitize tumor cells by metallic nanoparticles. However, though the “physics” behind nanoparticle-mediated radio-interaction has been well elaborated, practical applications in medicine remain challenging and often disappointing because of limited knowledge on biological mechanisms leading to cell damage enhancement and eventually cell death. In the present study, we analyzed the influence of different nanoparticle materials (platinum (Pt), and gold (Au)), cancer cell types (HeLa, U87, and SKBr3), and doses (up to 4 Gy) of low-Linear Energy Transfer (LET) ionizing radiation (γ- and X-rays) on the extent, complexity and reparability of radiation-induced γH2AX + 53BP1 foci, the markers of double stand breaks (DSBs). Firstly, we sensitively compared the focus presence in nuclei during a long period of time post-irradiation (24 h) in spatially (three-dimensionally, 3D) fixed cells incubated and non-incubated with Pt nanoparticles by means of high-resolution immunofluorescence confocal microscopy. The data were compared with our preliminary results obtained for Au nanoparticles and recently published results for gadolinium (Gd) nanoparticles of approximately the same size (2–3 nm). Next, we introduced a novel super-resolution approach—single molecule localization microscopy (SMLM)—to study the internal structure of the repair foci. In these experiments, 10 nm Au nanoparticles were used that could be also visualized by SMLM. Altogether, the data show that different nanoparticles may or may not enhance radiation damage to DNA, so multi-parameter effects have to be considered to better interpret the radiosensitization. Based on these findings, we discussed on conclusions and contradictions related to the effectiveness and presumptive mechanisms of the cell radiosensitization by nanoparticles. We also demonstrate that SMLM offers new perspectives to study internal structures of repair foci with the goal to better evaluate potential differences in DNA damage patterns.

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