Enhanced Transfection Efficiency of Multicomponent Lipoplexes in the Regime of Optimal Membrane Charge Density

2008 ◽  
Vol 112 (36) ◽  
pp. 11298-11304 ◽  
Author(s):  
Giulio Caracciolo ◽  
Daniela Pozzi ◽  
Ruggero Caminiti ◽  
Cristina Marchini ◽  
Maura Montani ◽  
...  
Keyword(s):  
Charge Density ◽  
Transfection Efficiency ◽  
Membrane Charge

New multivalent cationic lipids reveal bell curve for transfection efficiency versus membrane charge density: lipid-DNA complexes for gene delivery

2005 ◽  
Vol 7 (6) ◽  
pp. 739-748 ◽  
Author(s):  
Ayesha Ahmad ◽  
Heather M. Evans ◽  
Kai Ewert ◽  
Cyril X. George ◽  
Charles E. Samuel ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Charge Density ◽  
Transfection Efficiency ◽  
Cationic Lipids ◽  
Dna Complexes ◽  
Bell Curve ◽  
Membrane Charge

scholarly journals Anomalous Thermo-osmotic Conversion Performance of Ionic Covalent-Organic-Framework Membranes in Response to Charge Variations

2022 ◽  
Author(s):  
Qi Sun ◽  
Weipeng Xian ◽  
Xiuhui Zuo ◽  
Changjia Zhu ◽  
Qing Guo ◽  
...  
Keyword(s):  
Charge Density ◽  
Energy Conversion ◽  
Energy Demand ◽  
High Performance ◽  
Critical Factor ◽  
Energy Conversion Efficiency ◽  
Nanoporous Membranes ◽  
Power Extraction ◽  
Membrane Charge ◽  
Salinity Difference

Abstract The development of efficient thermo-osmotic energy conversion devices has fascinated scientists and engineers for several decades in terms of satisfying the growing energy demand. The fabrication of ionic membranes with a high charge population is known to be a critical factor in the design of high-performance power generators for achieving high permselectivity and, consequently, high power extraction efficiency. Herein, we experimentally demonstrated that the thermo-osmotic energy conversion efficiency was improved by increasing the membrane charge density; however, this enhancement occurred only within a narrow window and subsequently exhibited a plateau over a threshold density. The complex interplay between pore−pore interactions and fluid structuration for ion transport across the upscaled nanoporous membranes helped explain the obtained results with the aid of numerical simulations. Consequently, the power generation efficiency of the multipore membrane deteriorated, deviating considerably from the case of simple linear extrapolation of the behavior of the single-pore counterparts. A plateau in the output electric power was observed at a moderate charge density, affording a value of 210 W m−2 at a 50-fold salinity difference with a temperature gradient of 40 K. This study has far-reaching implications for discerning an optimal range of membrane charge populations for augmenting the energy extraction, rather than intuitively focusing on achieving high densities.

scholarly journals DNA–DOPE–gemini surfactants complexes at low surface charge density: from structure to transfection efficiency

2018 ◽  
Vol 37 (01) ◽  
pp. 57-69 ◽  
Author(s):  
Lukáš Hubčík ◽  
Dominika Galliková ◽  
Petra Pullmannová ◽  
Ľubica Lacinová ◽  
Zdena Sulová ◽  
...  
Keyword(s):  
Charge Density ◽  
Surface Charge ◽  
Surface Charge Density ◽  
Gemini Surfactants ◽  
Transfection Efficiency

scholarly journals Zeta Potential, Effective Membrane Charge and Donnan Potential for TiO2 NF Ceramic Membrane

2018 ◽  
Vol 24 (9) ◽  
pp. 21 ◽  
Author(s):  
Ahmed Faiq Hassan Al-Alawy ◽  
Amer Naji Ahmed Al-Naemi ◽  
Mudhaffar Yacoub Hussein
Keyword(s):  
Zeta Potential ◽  
Charge Density ◽  
Surface Charge ◽  
Isoelectric Point ◽  
Pore Radius ◽  
Ceramic Membrane ◽  
Effective Thickness ◽  
Donnan Potential ◽  
Membrane Charge ◽  
Effective Pore Radius

Nanofiltration (NF) ceramic membrane have found increasing applications particularly in wastewater and water treatment. In order to estimate and optimize the performance of NF membranes, the membrane should be characterized correctly in terms of their basic parameters such as effective pore radius (rp) and equivalent effective thickness as well as effective surface charge ( ), the effective charge density ( ) and Donnan potential ( ). The impact of electrokinetic (zeta) potential on the membrane surface charge density, effective membrane charge density and Donnan potential at two different concentrations of the reference solutions 0.001, 0.01 M sodium chloride at various pH values from 3 to 9, and effective pore radius (rp) for nominal 0.9 nm  ceramic TiO2 NF membrane were evaluated. Experiments were conducted at cross flow (1.0 m/s) using Microelectrophoresis technique for measuring membrane zeta potential, effective pore radius, and Donnan steric pore model (DSPM). The TiO2 membrane isoelectric point (net membrane charge equals zero) was found at pH of 3.7, 3.5 for 0.001 and 0.01 M NaCl respectively. The results showed that the NF membrane zeta potential changes its sign from positive to negative after the isoelectric point. The evaluated effective pore radius was found to be equal to 0.56 nm by using (DSPM) and the membrane equivalent effective thickness equals to (2×10-6 m).  

scholarly journals Nanogyroids Incorporating Multivalent Lipids: Enhanced Membrane Charge Density and Pore Forming Ability for Gene Silencing

Langmuir ◽  
2011 ◽  
Vol 27 (12) ◽  
pp. 7691-7697 ◽  
Author(s):  
Cecília Leal ◽  
Kai K. Ewert ◽  
Rahau S. Shirazi ◽  
Nathan F. Bouxsein ◽  
Cyrus R. Safinya
Keyword(s):  
Gene Silencing ◽  
Charge Density ◽  
Membrane Charge
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