Studies of Adsorption and Viscoelastic Properties of Proteins onto Liquid Crystal Phthalocyanine Surface Using Quartz Crystal Microbalance with Dissipation Technique

2008 ◽  
Vol 112 (31) ◽  
pp. 11822-11830 ◽  
Author(s):  
Sharmistha Paul ◽  
Deepen Paul ◽  
Tamara Basova ◽  
Asim K. Ray
Keyword(s):  
Liquid Crystal ◽  
Quartz Crystal Microbalance ◽  
Viscoelastic Properties ◽  
Quartz Crystal

Monitoring the Viscoelastic Properties of Skin in Liquid Environments Using Quartz Crystal Microbalance

2011 ◽  
Vol 100 (2) ◽  
pp. 530-535 ◽  
Author(s):  
N. Sanjeeva Murthy ◽  
Fahmi Bedoui ◽  
Brian E. Kilfoyle ◽  
Carmine Iovine ◽  
Bozena Michniak-Kohn ◽  
...  
Keyword(s):  
Quartz Crystal Microbalance ◽  
Viscoelastic Properties ◽  
Quartz Crystal

scholarly journals Quartz Crystal Microbalance with Dissipation Monitoring of Dynamic Viscoelastic Changes of Tobacco BY-2 Cells under Different Osmotic Conditions

Biosensors ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 136
Author(s):  
Zongxing Chen ◽  
Tiean Zhou ◽  
Jiajin Hu ◽  
Haifeng Duan
Keyword(s):  
Mechanical Properties ◽  
Quartz Crystal Microbalance ◽  
Viscoelastic Properties ◽  
Quartz Crystal ◽  
Morphological Changes ◽  
Turgor Pressure ◽  
Plant Cells ◽  
Dynamic Monitoring ◽  
Cellular Structures ◽  
Living Plant

The plant cell mechanics, including turgor pressure and wall mechanical properties, not only determine the growth of plant cells, but also reflect the functional and structural changes of plant cells under biotic and abiotic stresses. However, there are currently no appropriate techniques allowing to monitor the complex mechanical properties of living plant cells non-invasively and continuously. In this work, quartz crystal microbalance with dissipation (QCM-D) monitoring technique with overtones (3–9) was used for the dynamic monitoring of adhesions of living tobacco BY-2 cells onto positively charged N,N-dimethyl-N-propenyl-2-propen-1-aminiumchloride homopolymer (PDADMAC)/SiO2 QCM crystals under different concentrations of mannitol (CM) and the subsequent effects of osmotic stresses. The cell viscoelastic index (CVIn) (CVIn = ΔD⋅n/ΔF) was used to characterize the viscoelastic properties of BY-2 cells under different osmotic conditions. Our results indicated that lower overtones of QCM could detect both the cell wall and cytoskeleton structures allowing the detection of plasmolysis phenomena; whereas higher overtones could only detect the cell wall’s mechanical properties. The QCM results were further discussed with the morphological changes of the BY-2 cells by an optical microscopy. The dynamic changes of cell’s generated forces or cellular structures of plant cells caused by external stimuli (or stresses) can be traced by non-destructive and dynamic monitoring of cells’ viscoelasticity, which provides a new way for the characterization and study of plant cells. QCM-D could map viscoelastic properties of different cellular structures in living cells and could be used as a new tool to test the mechanical properties of plant cells.

scholarly journals Changes of Viscoelastic Properties of Aptamer-Based Sensing Layers Following Interaction with Listeria innocua

Sensors ◽  
2021 ◽  
Vol 21 (16) ◽  
pp. 5585
Author(s):  
Marek Tatarko ◽  
Sandro Spagnolo ◽  
Veronika Oravczová ◽  
Judit Süle ◽  
Milan Hun ◽  
...  
Keyword(s):  
Protein Structure ◽  
Shear Modulus ◽  
Resonant Frequency ◽  
Penetration Depth ◽  
Quartz Crystal Microbalance ◽  
Viscoelastic Properties ◽  
Quartz Crystal ◽  
Viscosity Coefficient ◽  
Listeria Innocua ◽  
E Coli

A multiharmonic quartz crystal microbalance (QCM) has been applied to study the viscoelastic properties of the aptamer-based sensing layers at the surface of a QCM transducer covered by neutravidin following interaction with bacteria Listeria innocua. Addition of bacteria in the concentration range 5 × 103–106 CFU/mL resulted in a decrease of resonant frequency and in an increase of dissipation. The frequency decrease has been lower than one would expect considering the dimension of the bacteria. This can be caused by lower penetration depth of the acoustics wave (approximately 120 nm) in comparison with the thickness of the bacterial layer (approximately 500 nm). Addition of E. coli at the surface of neutravidin as well as aptamer layers did not result in significant changes in frequency and dissipation. Using the Kelvin–Voight model the analysis of the viscoelastic properties of the sensing layers was performed and several parameters such as penetration depth, Γ, viscosity coefficient, η, and shear modulus, μ, were determined following various modifications of QCM transducer. The penetration depth decreased following adsorption of the neutravidin layer, which is evidence of the formation of a rigid protein structure. This value did not change significantly following adsorption of aptamers and Listeria innocua. Viscosity coefficient was higher for the neutravidin layer in comparison with the naked QCM transducer in a buffer. However, a further increase of viscosity coefficient took place following attachment of aptamers suggesting their softer structure. The interaction of Listeria innocua with the aptamer layer resulted in slight decrease of viscosity coefficient. The shearing modulus increased for the neutravidin layer and decreased following aptamer adsorption, while a slight increase of µ was observed after the addition of Listeria innocua.

Measurements of fast fluctuations of viscoelastic properties with the quartz crystal microbalance

The Analyst ◽  
2005 ◽  
Vol 130 (11) ◽  
pp. 1474 ◽  
Author(s):  
Markus Pax ◽  
Janosch Rieger ◽  
Robert H. Eibl ◽  
Christiane Thielemann ◽  
Diethelm Johannsmann
Keyword(s):  
Quartz Crystal Microbalance ◽  
Viscoelastic Properties ◽  
Quartz Crystal
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