Three-Dimensional Molecular Mapping of a Multiple Emulsion by Means of CARS Microscopy

2008 ◽  
Vol 112 (5) ◽  
pp. 1420-1426 ◽  
Author(s):  
Tobias Meyer ◽  
Denis Akimov ◽  
Nicolae Tarcea ◽  
Susana Chatzipapadopoulos ◽  
Gerald Muschiolik ◽  
...  
Keyword(s):  
Molecular Mapping ◽  
Three Dimensional ◽  
Multiple Emulsion ◽  
Cars Microscopy

scholarly journals Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging

2008 ◽  
Vol 33 (16) ◽  
pp. 1887 ◽  
Author(s):  
Tom Robinson ◽  
Prashant Valluri ◽  
Hugh B. Manning ◽  
Dylan M. Owen ◽  
Ian Munro ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Molecular Mapping ◽  
Three Dimensional ◽  
Fluorescence Lifetime Imaging ◽  
Microfluidic Mixing ◽  
Lifetime Imaging

Vive La Différence! Mapping Macromolecular Complexes by Generalized Difference Imaging

2000 ◽  
Vol 6 (S2) ◽  
pp. 252-253
Author(s):  
Alasdair C. Steven ◽  
James F. Conway ◽  
Naiqian Cheng ◽  
Norman R. Watts ◽  
Paul T. Wingfield
Keyword(s):  
Molecular Mapping ◽  
Three Dimensional ◽  
Cellular Functions ◽  
Macromolecular Complexes ◽  
Protein Subunits ◽  
First Order ◽  
Molecular Weights ◽  
Cryo Electron Microscopy ◽  
Polypeptide Chains ◽  
Difference Imaging

Overview. Three-dimensional cryo-electron microscopy holds great potential for the investigation of macromolecular complexes, including viruses, the cytoskeleton, and the nanomachines that carry out many vital cellular functions. Such complexes often contain protein subunits of different kinds, with cumulative molecular weights running well into the megadalton range. To rationalize their functional assignments in structural terms, the first order of business is to map the locations of the component subunits of a given complex. A more detailed objective then becomes to understand the organization of their domains and the overall layout of the polypeptide chains. With current methodology, resolutions below 20Å are often achievable in cryo-EM, extending below 10 Å in favorable cases. Although resolutions of this order yield valuable morphological information, they do not usually suffice to identify individual subunits within a complex. Generalized difference imaging offers a solution to this problem of molecular mapping.

Abstract 18171: HCN Channel Distribution in the Human Sinoatrial Node and Latent Atrial Pacemakers (Best of Basic Science Abstract)

Circulation ◽  
2015 ◽  
Vol 132 (suppl_3) ◽  
Author(s):  
Ning Li ◽  
Thomas A Csepe ◽  
Brian J Hansen ◽  
Halina Dobrzynski ◽  
Robert S Higgins ◽  
...  
Keyword(s):  
Molecular Mapping ◽  
Expression Patterns ◽  
Critical Role ◽  
Density Ratio ◽  
Three Dimensional ◽  
Cardiac Pacemaker ◽  
Right Atrial ◽  
Expression Ratio ◽  
Compact Region ◽  
Fresh Frozen

Background: The hyperpolarization-activated current, I f , plays an important role in cardiac pacemaker cells. However, the distribution and expression of HCN isoforms in the human SAN and latent atrial pacemaker clusters is unknown. Methods: Human atria and entire SAN complexes were isolated from failing (n=5) and non-failing (n=9) human hearts cardioplegically-arrested in the operating room. Three dimensional intramural SAN structure was identified as the fibrotic compact region around the SAN artery with Connexin43-negative pacemaker cardiomyocytes visualized in Masson’s trichrome and immunostained cryosections. Pure SAN tissue was precisely isolated from the frozen SAN cryo blocks using a 16G biopsy needle (Figure). Atrial tissues from different locations were fresh frozen in liquid nitrogen. Immunoblot and immunostaining were used to study the expression pattern of HCN isoforms. Results: Three HCN isoform proteins were detected in the atria and SAN (Figure). HCN1 was predominantly distributed in all the human SAN with a 125.1±40.2 (n=12) expression ratio of SAN to right atrial free wall (RAFW). HCN2 and HCN4 expression levels were higher in SAN than atria with ratios of 6.1±0.9 and 4.6±0.6 (n=12), respectively. HCN2 expression but not HCN1/4 in the latent atrial pacemakers from the right atrioventricular ring (RR) area was significantly higher than in RAFW, with the band density ratio of RR/RAFW= 2.4±0.4 (n=12). Conclusions: This is the first study to conduct precise molecular mapping of the human SAN by isolating pure pacemaker SAN tissue. HCN1 was almost exclusively expressed in SAN and may play a critical role in determining the leading pacemaker in human SAN. Even HCN2 and HCN4 expression is higher in the SAN than RAFW, these isoforms are less SAN-specific than HCN1. SAN and latent atrial pacemakers have different HCN expression patterns, suggesting the utility of HCN isoform specific blockers to selectively modify sinus rhythm or atrial ectopic rhythms.

Three-Dimensional Molecular Mapping of Ionic Liquids at Electrified Interfaces

ACS Nano ◽  
2020 ◽  
Vol 14 (12) ◽  
pp. 17515-17523
Author(s):  
Shan Zhou ◽  
Kaustubh S. Panse ◽  
Mohammad Hossein Motevaselian ◽  
Narayana R. Aluru ◽  
Yingjie Zhang
Keyword(s):  
Ionic Liquids ◽  
Molecular Mapping ◽  
Three Dimensional ◽  
Electrified Interfaces

scholarly journals Imaging Viral Factories

Proceedings ◽  
2020 ◽  
Vol 50 (1) ◽  
pp. 3
Author(s):  
Cristina Risco Ortiz
Keyword(s):  
Electron Microscopy ◽  
Viral Replication ◽  
Molecular Mapping ◽  
Light And Electron Microscopy ◽  
Three Dimensional ◽  
Three Dimensions ◽  
Particle Assembly ◽  
Intracellular Compartments ◽  
Cell Organization ◽  
Viral Lifecycle

Viruses remodel cellular compartments to build their replication factories. Remarkably, viruses are also able to induce new membranes and new organelles. As a result of recent advances in light and transmission electron microscopy (TEM), we are starting to become aware of the variety of structures that viruses assemble inside cells. Viral factories are intracellular compartments harboring replication organelles that contain viral replication complexes and the sites of virus particle assembly. This lecture will revise the most relevant imaging technologies for studying the biogenesis of viral replication factories. Live cell microscopy, correlative light and electron microscopy, cryo-TEM, and three-dimensional imaging methods are unveiling how viruses manipulate cell organization. In particular, methods for molecular mapping in situ, in two and three dimensions, are revealing how macromolecular complexes build functional replication complexes inside infected cells. The combination of all these imaging approaches is uncovering the viral lifecycle events with a detail never seen before.

Unraveling the complexity of deep gas accumulations with three-dimensional multimodal CARS microscopy

Geology ◽  
2012 ◽  
Vol 40 (12) ◽  
pp. 1063-1066 ◽  
Author(s):  
Robert C. Burruss ◽  
Aaron D. Slepkov ◽  
Adrian F. Pegoraro ◽  
Albert Stolow
Keyword(s):  
Three Dimensional ◽  
Cars Microscopy

The orbit of Pluto over a long interval of time

1966 ◽  
Vol 25 ◽  
pp. 227-229 ◽  
Author(s):  
D. Brouwer
Keyword(s):  
Periodic Orbit ◽  
Numerical Integration ◽  
Restricted Problem ◽  
Three Dimensional ◽  
The Third ◽  
Circular Restricted Problem ◽  
Resonance Relation

The paper presents a summary of the results obtained by C. J. Cohen and E. C. Hubbard, who established by numerical integration that a resonance relation exists between the orbits of Neptune and Pluto. The problem may be explored further by approximating the motion of Pluto by that of a particle with negligible mass in the three-dimensional (circular) restricted problem. The mass of Pluto and the eccentricity of Neptune's orbit are ignored in this approximation. Significant features of the problem appear to be the presence of two critical arguments and the possibility that the orbit may be related to a periodic orbit of the third kind.

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