Probing the Enzymatic Activity of Alkaline Phosphatase within Quantum Dot Bioconjugates

2015 ◽  
Vol 119 (4) ◽  
pp. 2208-2221 ◽  
Author(s):  
Jonathan C. Claussen ◽  
Anthony Malanoski ◽  
Joyce C. Breger ◽  
Eunkeu Oh ◽  
Scott A. Walper ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Quantum Dot ◽  
Enzymatic Activity

Soil response to chemicals used in a field experiment

2013 ◽  
Vol 27 (2) ◽  
pp. 151-158 ◽  
Author(s):  
S. Jezierska-Tys ◽  
A. Rutkowska
Keyword(s):  
Alkaline Phosphatase ◽  
Enzymatic Activity ◽  
Field Experiment ◽  
Soil Microorganisms ◽  
Block Design ◽  
Soil Samples ◽  
Alkaline Phosphatases ◽  
Soil Response ◽  
Negative Effect ◽  
Set Up

Abstract The effect of chemicals (Reglone 200 SL and Elastiq 550 EC) on soil microorganisms and their enzymatic activity was estimated. The study was conducted in a field experiment which was set up in the split-block design and comprised three treatments. Soil samples were taken six times, twice in each year of study. The results showed that the application of chemicals generally had no negative effect on the number of soil microorganisms. The application of Reglone 200 SL caused an increase of proteolytic and ureolytic activity and affected the activity of dehydrogenases, acid and alkaline phosphatases in the soil. The soil subjected of Elastiq 550 EC was characterized by lower activity of dehydrogenases, protease, urease and alkaline phosphatase.

scholarly journals Replacing Maize Grain with Ancient Wheat Lines By-Products in Organic Laying Hens’ Diet Affects Intestinal Morphology and Enzymatic Activity

2021 ◽  
Vol 13 (12) ◽  
pp. 6554
Author(s):  
Nicola Francesco Addeo ◽  
Basilio Randazzo ◽  
Ike Olivotto ◽  
Maria Messina ◽  
Francesca Tulli ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Enzymatic Activity ◽  
Laying Hens ◽  
Triticum Aestivum L ◽  
Treated Group ◽  
Intestinal Morphology ◽  
Control Group ◽  
Positive Effects ◽  
Maize Grain ◽  
By Products

The effects of replacement of maize grain with ancient wheat by-products on intestinal morphometry and enzymatic activity in laying hens was studied. Eighty hens were divided into two groups (40 each, 8 replicates, 5 hens/replicate) fed two isoproteic and isoenergetic diets. In the treated group, part of the maize was replaced by a mix of ancient grains (AGs) middling, in a 50:50 ratio of Triticum aestivum L. var. spelta (spelt) and Triticum durum dicoccum L. (emmer wheat). The AG diet affected the weight of all the large intestine tracts, decreasing the weight of caeca (p < 0.01) and increasing those of colon (p < 0.01), rectum and cloaca (p < 0.05). Villus height in the AG group was higher (p < 0.01) than the control for the duodenum and jejunum, while for the ileum, the control group showed the highest values (p < 0.01). The submucosa thickness was higher (p < 0.01) in the control group for the duodenum and ileum, while the jejunum for the AG group showed the highest (p < 0.05) submucosa thickness. The crypts depth was higher (p < 0.01) in the control group for the duodenum and ileum. Enzyme activity was enhanced by AGs (p < 0.01) in the duodenum. Regarding the jejunum, sucrase-isomaltase and alkaline phosphatase had higher activity (p < 0.05 and p < 0.01, respectively) in the AG group. In the ileum, sucrase-isomaltase showed higher activity (p < 0.01) in the control group, while alkaline phosphatase showed the highest values (p < 0.05) in the AG group. Overall, results suggested that the dietary inclusion of AGs exerted positive effects in hens, showing an improved intestinal function.

scholarly journals Necessity of Enzymatic Activity of Alkaline Phosphatase for Mineralization of Osteoblastic Cells

2002 ◽  
Vol 88 (3) ◽  
pp. 262-269 ◽  
Author(s):  
Yuki Sugawara ◽  
Kuniaki Suzuki ◽  
Mino Koshikawa ◽  
Masaki Ando ◽  
Junichiro Iida
Keyword(s):  
Alkaline Phosphatase ◽  
Enzymatic Activity ◽  
Osteoblastic Cells

scholarly journals The effect of different copper doses and organic fertilisation on soil’s enzymatic activity

2020 ◽  
Vol 66 (No. 2) ◽  
pp. 93-98
Author(s):  
Beata Kuziemska ◽  
Andrzej Wysokiński ◽  
Joanna Trębicka
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Enzymatic Activity ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Organic Materials ◽  
Chicken Manure ◽  
Test Plant ◽  
Orchard Grass ◽  
Organic Fertilisation

A three-year pot experiment carried out in the vegetation hall in 2014–2016 included studying the enzymatic activity of soil, into which various amounts of copper: (100, 200 and 300 mg Cu/kg soil) and organic materials (cattle manure, chicken manure, post-mushroom substrate) were introduced, used separately, at a soil-introduction dose of 2 g C<sub>org</sub>/kg. Copper and organic materials were used once, only in the first year of the study, before sowing test plant orchard grass. In soil collected after the last (fourth) swath of grass in each year of the study, the activity of urease, dehydrogenases, acid, and alkaline phosphatase was determined. Applications of copper to the soil, regardless of its dose, resulted in a decrease in urease, dehydrogenases and alkaline phosphatase and an increase in acid phosphatase activity. The inactivating effect of this metal on the activity of urease, dehydrogenases and alkaline phosphatase increased with the increase of its dose. Organic fertilisation generally increased the enzymatic activity of the analysed soil. In subsequent years of the study, urease and alkaline phosphatase activity decreased, while acid phosphatase activity increased. Dehydrogenase activity did not change significantly in subsequent years of the study.  

Mercury and other potentially toxic elements in the Sierra Gorda (Queretaro area, Mexico): affection to enzymatic activity in soils.

2020 ◽  
Author(s):  
Pablo Higueras ◽  
Karen Arroyo ◽  
JuanAntonio Campos ◽  
Jesus Peco ◽  
JoseMaria Esbrí ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Enzymatic Activity ◽  
Contaminated Soils ◽  
Toxic Elements ◽  
Soil Health ◽  
Potentially Toxic Elements ◽  
Key Factors ◽  
Permissible Limits ◽  
Study Sites

&lt;p&gt;Cinnabar mining, to obtain mercury, is still an important activity for the residents of the Sierra Gorda in Mexico, so this activity is currently source of mercury emission and possibly of other potentially toxic elements (PTE). In this work, seven study sites, located in areas with presence of exploitations of active or decommissioned mercury mines, have been studies with the aim of characterizing its occurrence and their effects on soil health.&lt;/p&gt;&lt;p&gt;Biogeochemical analyses have been carried out with the purpose of identifying the key factors related with nutritional and toxicological status of these soils, looking for possible relationships between mercury, PTEs and their impact on the enzymatic activity of the soil.&lt;/p&gt;&lt;p&gt;The values &amp;#8203;&amp;#8203;obtained for total mercury ranged from 5 to 159 ppm; comparing these values with those from an uncontaminated area, we observe that all zones are above reference range (0.01 to 0.03 mg/kg) and that four of them exceed the maximum permissible limits (23 mg/kg), according to Mexican regulations. Other measured PTE elements were Pb, with a range between 18.7 to 814.1 mg/kg; Cu between 45.4 to 94.2 mg/kg; Zn between 145.1 to 555.8 mg/kg; As between 30.5 to 1590 mg/kg; and Sb between 18.3 to 169.6 mg/kg. &amp;#160;Comparing with other areas, anomalous concentrations of trace elements in soils with the following values are considered: Pb up to 10,000 mg/kg, Cu up to 2,000 mg/kg, Zn up to 10,000 mg/kg and As up to 2500 mg/kg; none of the determined elements exceeds these reference values. In the case of enzymatic activities, a range between 111.36 and 332.38 &amp;#181;gTPF g&lt;sup&gt;-1&lt;/sup&gt;day&lt;sup&gt;-1&lt;/sup&gt; was obtained with dehydrogenase. These values are slightly higher compared to other Hg contaminated soils (110 &amp;#181;gTPF g&lt;sup&gt;-1&lt;/sup&gt;day&lt;sup&gt;-1&lt;/sup&gt;) described by this team. For the acid phosphatase, a range between 516.72 to 1606.34 &amp;#181;gPNF g&lt;sup&gt;-1&lt;/sup&gt;h&lt;sup&gt;-1&lt;/sup&gt;; and for alkaline phosphatase a range between 1624.92 to 4070.82 &amp;#181;gPNF g&lt;sup&gt;-1&lt;/sup&gt;h&lt;sup&gt;-1&lt;/sup&gt;. These values correspond to those measured in Sokolov, Czech Republic, ranging from 381 to 1510 &amp;#181;gPNF g&lt;sup&gt;-1&lt;/sup&gt;h&lt;sup&gt;-1&lt;/sup&gt; for acid phosphatase and 455 to 4820 &amp;#181;gPNF g&lt;sup&gt;-1&lt;/sup&gt;h&lt;sup&gt;-1&lt;/sup&gt; for alkaline phosphatase measured in topsoil layer from spoil heaps after brown coal mining.&lt;/p&gt;&lt;p&gt;Our results show that the soil has contents of PTE elements indicating low pollution degree, except for Hg, registering concentrations above the maximum permissible limits for non-industrial soils; however, the results of the enzymatic activity reflect a &quot;good&quot; activity. Therefore, the incidence of the presence of these metals in the soil health, as measured through enzymatic activity, does not have a significant impact and the studied soils can be considered as suitable for commercial, residential or agricultural uses.&lt;/p&gt;

scholarly journals Analysis of Localization of Mutated Tissue-Nonspecific Alkaline Phosphatase Proteins Associated with Neonatal Hypophosphatasia Using Green Fluorescent Protein Chimeras1

1998 ◽  
Vol 83 (11) ◽  
pp. 3936-3942
Author(s):  
Guiming Cai ◽  
Toshimi Michigami ◽  
Takehisa Yamamoto ◽  
Natsuo Yasui ◽  
Kenichi Satomura ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Plasma Membrane ◽  
Green Fluorescent Protein ◽  
Enzymatic Activity ◽  
Fluorescent Protein ◽  
Clinical Severity ◽  
Wild Type ◽  
Neonatal Patient ◽  
Tissue Nonspecific Alkaline Phosphatase ◽  
Green Fluorescent

Hypophosphatasia is associated with a defect of the tissue-nonspecific alkaline phosphatase (TNSALP) gene. The onset and clinical severity are usually correlated in hypophosphatasia; patients with perinatal hypophosphatasia die approximately at the time of birth. In contrast, we describe a male neonatal patient with hypophosphatasia who had no respiratory problems and survived. He was compound heterozygous for the conversion of Phe to Leu at codon 310 (F310L) and the deletion of a nucleotide T at 1735 (delT1735), causing the frame shift with the result of the addition of 80 amino acids at the C-terminal of the protein. Because the C-terminal portion of TNSALP is known to be important for TNSALP to bind to the plasma membrane, the localization of wild-type and mutated TNSALP proteins was analyzed using green fluorescent protein chimeras. The expression vectors containing the complementary DNA of fusion proteins consisting of signal peptide, green fluorescent protein, and wild-type or mutated TNSALP, caused by delT1735 or F310L mutation, were introduced transiently or stably in Saos-2 cells. The delT1735 mutant failed to localize at the cell surface membrane, whereas the wild-type and the F310L mutants were located in the plasma membrane and cytoplasm. The assay for enzymatic activity of TNSALP revealed that the delT1735 mutant lost the activity and that the F310L mutant exhibited an enzymatic activity level that was 72% of the normal level. The F310L mutation was also detected in another neonatal patient with relatively mild (nonlethal) hypophosphatasia (reported in J Clin Endocrinol Metab, 81:4458–4461, 1996), suggesting that residual ALP activity of the F310L mutant contributes to the less severe phenotype. The patient is unique, with respect to a discrepancy between onset and clinical severity in hypophosphatasia.

Size-dependent CdSe quantum dot–lysozyme interaction and effect on enzymatic activity

RSC Advances ◽  
2016 ◽  
Vol 6 (52) ◽  
pp. 46744-46754 ◽  
Author(s):  
Kishan Das ◽  
Kamla Rawat ◽  
Rajan Patel ◽  
H. B. Bohidar
Keyword(s):  
Quantum Dots ◽  
Quantum Dot ◽  
Enzymatic Activity ◽  
Cdse Quantum Dots ◽  
Size Dependent ◽  
Cdse Quantum Dot

Size-dependent (2.5 and 6.3 nm) interaction of MPA modified hydrophilic CdSe quantum dots with lysozyme are reported.

scholarly journals Histochemical localization of potassium-stimulated P-nitrophenylphosphatase activity in the somatosensory cortex of the rat.

1976 ◽  
Vol 24 (6) ◽  
pp. 731-739 ◽  
Author(s):  
W L Stahl ◽  
S H Broderson
Keyword(s):  
Alkaline Phosphatase ◽  
Enzymatic Activity ◽  
Somatosensory Cortex ◽  
Adenosine Triphosphatase ◽  
Large Diameter ◽  
Cortical Layers ◽  
Outer Portion ◽  
Cacodylate Buffer ◽  
Neuronal Processes ◽  
Rat Somatosensory Cortex

Potassium-stimulated p-nitrophenylphosphatase (K+-pNPPase) activity was investigated in rat somatosensory cortex where 64-88% of enzymatic activity survived 5-10 min of fixation with 3% formaldehyde in 0.1 M cacodylate buffer, pH 7.4. Potassium-stimulated activity was inhibited by 1-10 mM ouabain. Levamisole (1.7 mM) inhibited brain alkaline phosphatase activity, facilitating the detection of K+-pNPPase activity. Strontium (10-20 mM) inhibited enzymatic activity by 38-75%. In parallel histochemical studies reaction product was found in strata, with cortical layers 2, 3, 4 and the outer portion of 5 containing the heaviest deposits. Highly reactive, vertically oriented, large diameter fibers were seen as groups between the outer portion of layer 5 and the pail surface. These fibers apparently arborize in the superficial layers. Smaller fibers were also positive and were oriented in various planes. The highest density of smaller, positive fibers occurred in layers 2 through 5. All positive fibers appeared to be axons or dendrites. Reaction product was not heavily concentrated in neuron perikarya or in glial elements. Sections did not contain reaction product when incubated in media lacking K+ or containing ouabain. The convergence of data from parallel histochemical and biochemical approaches supports the conclusion that the reactivity localized in the cerebral cortex represented the site of K+-pNPPase, a known component of the Na+,K+-adenosine triphosphatase complex. Neuronal processes demonstrated the highest enzymatic activity and may be most important in the active transport of Na+ and K+ in somatosensory cortex.

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