Local Pressure Changes in Lipid Bilayers Due to Adsorption of Melittin and Magainin-h2 Antimicrobial Peptides: Results from Computer Simulations

2014 ◽  
Vol 118 (44) ◽  
pp. 12673-12679 ◽  
Author(s):  
Ardeshir Goliaei ◽  
Kolattukudy P. Santo ◽  
Max L. Berkowitz
Keyword(s):  
Antimicrobial Peptides ◽  
Lipid Bilayers ◽  
Computer Simulations ◽  
Local Pressure ◽  
Pressure Changes

Changes in intestinal volume with hemorrhage

1960 ◽  
Vol 199 (3) ◽  
pp. 589-592 ◽  
Author(s):  
Paul C. Johnson
Keyword(s):  
Small Intestine ◽  
Blood Volume ◽  
Sympathetic Nerve Activity ◽  
Venous Pressure ◽  
Volume Changes ◽  
Local Pressure ◽  
Local Reduction ◽  
Pressure Changes ◽  
Sympathetic Discharge ◽  
Intestinal Weight

The purpose of these experiments was to study the changes in intestinal volume occurring with hemorrhage, utilizing a gravimetric technique which permitted a study of small segments of the intestine. It had been observed previously that intestinal weight often increased in the upper small intestine during hemorrhage, while in the lower small intestine it usually decreased. In studying the latter effect it was found that sympathetic nerve activity and reduction of venous pressure were both important in decreasing intestinal volume. Changes in tonus and local reduction in arterial pressure did not appear to be important. The increase in volume with hemorrhage appeared due to epinephrine discharge from the adrenal medulla since it was eliminated by adrenalectomy. Local pressure changes and alteration of tonus were eliminated as causal factors. It appears that systemic hypotension induces sympathetic discharge which in turn may cause either an increase or a decrease in intestinal blood volume. Sympathetic discharge over the vasoconstrictor fibers reduces blood volume while adrenal medullary secretion increases it. The observed response is apparently a resultant of these two antagonistic effects.

Heterodimer Formation between the Antimicrobial Peptides Magainin 2 and PGLa in Lipid Bilayers:  A Cross-Linking Study†

Biochemistry ◽  
2001 ◽  
Vol 40 (41) ◽  
pp. 12395-12399 ◽  
Author(s):  
Toshiaki Hara ◽  
Yasuyuki Mitani ◽  
Kyoko Tanaka ◽  
Natsuko Uematsu ◽  
Asako Takakura ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Lipid Bilayers ◽  
Cross Linking

scholarly journals Immobilized artificial membrane (IAM) liquid chromatography as a model for antimicrobial peptide partitioning into cell membranes: An evaluation

Eureka ◽  
2010 ◽  
Vol 1 (1) ◽  
pp. 20-33
Author(s):  
Matthew Benesch ◽  
Ruthvnen Lewis ◽  
Ronald McElhaney
Keyword(s):  
Liquid Chromatography ◽  
Antimicrobial Peptides ◽  
Lipid Bilayers ◽  
Screening Method ◽  
Interfacial Region ◽  
Artificial Membrane ◽  
Lipid Packing ◽  
Immobilized Artificial Membrane ◽  
Fast Screening ◽  
Packing Densities

Non-covalent immobilized artificial membrane reverse-phase high performance liquid chromatography was previously evaluated as a means whereby elution times for antimicrobial peptides from columns mimicking the lipid bilayers of different membrane systems might be used as a fast-screening method to compare relative binding effectiveness. Such a system would aid in the development of antimicrobial peptides that bind preferentially to model pathogenic systems and leave the host’s membranes reasonably unaffected. A non-covalent approach allows for flexibility in membrane composition but was found to be inadequate for analysis of most peptides due to significant lipid loss at high acetonitrile concentrations. A covalent approach where phosphatidylcholine was amide-linked to the silica surface was examined to evaluate its use as a fast-screening method and compare its data to that collected from the non-covalent columns. Initial work with a 1-cm column proved ineffective due to problems with balancing flow rates with retention times, and work was shifted to a longer 10-cm column. Results suggested that peptides bind much more strongly to covalent columns than non-covalent ones, with the binding especially enhanced by the presence of cationic residues. These columns had lipid packing densities much lower than true membranes, indicating that the peptides were partitioning deep into the bonded phase of the columns rather than into the interfacial region of the phosphate head groups, as expected in situations of biologically-relevant lipid packing densities.

scholarly journals Morphological Changes Induced by the Action of Antimicrobial Peptides on Supported Lipid Bilayers

2011 ◽  
Vol 115 (1) ◽  
pp. 158-167 ◽  
Author(s):  
Ahmad Arouri ◽  
Volker Kiessling ◽  
Lukas Tamm ◽  
Margitta Dathe ◽  
Alfred Blume
Keyword(s):  
Antimicrobial Peptides ◽  
Lipid Bilayers ◽  
Morphological Changes ◽  
Supported Lipid Bilayers

scholarly journals Formation of adhesion domains in stressed and confined membranes

Soft Matter ◽  
2015 ◽  
Vol 11 (19) ◽  
pp. 3780-3785 ◽  
Author(s):  
Nadiv Dharan ◽  
Oded Farago
Keyword(s):  
Surface Tension ◽  
Lipid Bilayers ◽  
Computer Simulations ◽  
Molecular Model ◽  
Coarse Grained ◽  
Supported Lipid Bilayers

We use computer simulations of a coarse-grained molecular model of supported lipid bilayers to study the formation of adhesion domains in confined membranes, and in membranes subjected to a non-vanishing surface tension. When the membrane is subjected to compression, the condensation of the adhesion domains triggers membrane buckling.

scholarly journals Lysylated phospholipids stabilize models of bacterial lipid bilayers and protect against antimicrobial peptides

2014 ◽  
Vol 1838 (9) ◽  
pp. 2198-2204 ◽  
Author(s):  
Elizabeth Cox ◽  
Austen Michalak ◽  
Sarah Pagentine ◽  
Pamela Seaton ◽  
Antje Pokorny
Keyword(s):  
Antimicrobial Peptides ◽  
Lipid Bilayers ◽  
Bacterial Lipid

Synergistic effects of magainin 2 and PGLa on their heterodimer formation, aggregation, and insertion into the bilayer

RSC Advances ◽  
2015 ◽  
Vol 5 (3) ◽  
pp. 2047-2055 ◽  
Author(s):  
Eol Han ◽  
Hwankyu Lee
Keyword(s):  
Molecular Dynamics ◽  
Antimicrobial Peptides ◽  
Molecular Dynamics Simulations ◽  
Lipid Bilayers ◽  
Synergistic Effects ◽  
Coarse Grained ◽  
Dynamics Simulations ◽  
Coarse Grained Molecular Dynamics

We performed coarse-grained molecular dynamics simulations of antimicrobial peptides PGLa and magainin 2 in lipid bilayers.

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