Dependence of Purple Membrane Bump Curvature on pH and Ionic Strength Analyzed Using Atomic Force Microscopy Combined with Solvent Exchange

2014 ◽  
Vol 118 (31) ◽  
pp. 9322-9328 ◽  
Author(s):  
Yasunori Yokoyama ◽  
Kosuke Yamada ◽  
Yosuke Higashi ◽  
Satoshi Ozaki ◽  
Haorang Wang ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Ionic Strength ◽  
Purple Membrane ◽  
Solvent Exchange ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals 2P107 Curvature of Bump Structures on Purple Membrane Depending on pH and Ionic Strength Analyzed by Atomic Force Microscopy(03. Membrane proteins,Poster)

2013 ◽  
Vol 53 (supplement1-2) ◽  
pp. S176
Author(s):  
Yasunori Yokoyama ◽  
Kosuke Yamada ◽  
Yosuke Higashi ◽  
Satoshi Ozaki ◽  
Haorang Wang ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Ionic Strength ◽  
Membrane Proteins ◽  
Purple Membrane ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Contact-Mode High-Resolution High-Speed Atomic Force Microscopy Movies of the Purple Membrane

2009 ◽  
Vol 97 (5) ◽  
pp. 1354-1361 ◽  
Author(s):  
Ignacio Casuso ◽  
Noriyuki Kodera ◽  
Christian Le Grimellec ◽  
Toshio Ando ◽  
Simon Scheuring
Keyword(s):  
Atomic Force Microscopy ◽  
High Resolution ◽  
High Speed ◽  
Purple Membrane ◽  
Contact Mode ◽  
Force Microscopy ◽  
Atomic Force

Atomic Force Microscopy of Humic Substances:  Effects of pH and Ionic Strength

1999 ◽  
Vol 33 (21) ◽  
pp. 3911-3917 ◽  
Author(s):  
Eric Balnois ◽  
Kevin J. Wilkinson ◽  
Jamie R. Lead ◽  
Jacques Buffle
Keyword(s):  
Atomic Force Microscopy ◽  
Ionic Strength ◽  
Humic Substances ◽  
Force Microscopy ◽  
Atomic Force ◽  
Effects Of Ph

scholarly journals Immuno-atomic force microscopy of purple membrane

1996 ◽  
Vol 70 (4) ◽  
pp. 1796-1802 ◽  
Author(s):  
D.J. Müller ◽  
C.A. Schoenenberger ◽  
G. Büldt ◽  
A. Engel
Keyword(s):  
Atomic Force Microscopy ◽  
Purple Membrane ◽  
Force Microscopy ◽  
Atomic Force

Elastic modulus of suspended purple membrane measured by atomic force microscopy

2008 ◽  
Vol 254 (23) ◽  
pp. 7877-7880 ◽  
Author(s):  
Ari M. Siitonen ◽  
Koji Sumitomo ◽  
Chandra S. Ramanujan ◽  
Youichi Shinozaki ◽  
Nahoko Kasai ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Elastic Modulus ◽  
Purple Membrane ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Atomic force microscopy of native purple membrane

2000 ◽  
Vol 1460 (1) ◽  
pp. 27-38 ◽  
Author(s):  
Daniel J. Müller ◽  
J.Bernard Heymann ◽  
Filipp Oesterhelt ◽  
Clemens Möller ◽  
Hermann Gaub ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Purple Membrane ◽  
Force Microscopy ◽  
Atomic Force

Agarose gel structure using atomic force microscopy: Gel concentration and ionic strength effects

1998 ◽  
Vol 19 (10) ◽  
pp. 1606-1610 ◽  
Author(s):  
Mounir Maaloum ◽  
Nadine Pernodet ◽  
Bernard Tinland
Keyword(s):  
Atomic Force Microscopy ◽  
Ionic Strength ◽  
Agarose Gel ◽  
Gel Structure ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Atomic force microscopy of gastric mucin and chitosan mucoadhesive systems

2000 ◽  
Vol 348 (3) ◽  
pp. 557-563 ◽  
Author(s):  
Matthew P. DEACON ◽  
Simon McGURK ◽  
Clive J. ROBERTS ◽  
Phillip M. WILLIAMS ◽  
Saul J. B. TENDLER ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Atomic Force Microscopy ◽  
Ionic Strength ◽  
Average Length ◽  
Gastric Mucin ◽  
Acetate Buffer ◽  
Force Microscopy ◽  
Atomic Force ◽  
Molecular Hydrodynamic ◽  
Hydrodynamic Data

Atomic force microscopy has been utilized to probe, at a molecular level, the interaction between purified pig gastric mucin (PGM) and a mucoadhesive cationic polymer, chitosan (sea cure 210+), with a low degree (approx. 11%) of acetylation. Images were produced detailing the structures of both PGM and chitosan in 0.1 M acetate buffer (pH 4.5), followed by the complex of the two structures in the same buffer. PGM in 0.1 M acetate buffer revealed long linear filamentous structures, consistent with earlier electron microscopy and scanning tunnelling micoscopy studies. The chitosan molecules also adopted a linear conformation in the same buffer, although with a smaller average length and diameter. They appeared to adopt a stiff-coil conformation consistent with earlier hydrodynamic measurements. The complexes formed after mixing PGM and chitosan together revealed large aggregates. In 0.1 M ionic strength buffer they were of the order of 0.7 μm in diameter, consistent with previous electron microscopy studies. The effect of ionic strength of the buffer on the structure of the complex was also studied and, together with molecular hydrodynamic data, demonstrates that the interaction is principally electrostatic in nature.

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