Peptide Nucleic Acid-Mediated Aggregation of Reduced Graphene Oxides and Label-Free Detection of DNA Mutation

2014 ◽  
Vol 118 (23) ◽  
pp. 6297-6301 ◽  
Author(s):  
Taegyeong Kang ◽  
Hoon Choi ◽  
Sang-Woo Joo ◽  
So Yeong Lee ◽  
Kyong-Ah Yoon ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Label Free ◽  
Graphene Oxides ◽  
Dna Mutation ◽  
Reduced Graphene ◽  
Label Free Detection ◽  
Reduced Graphene Oxides

scholarly journals Label-Free Detection of Zeptomol miRNA via Peptide Nucleic Acid Hybridization Using Novel Cyclic Voltammetry Method

Sensors ◽  
2020 ◽  
Vol 20 (3) ◽  
pp. 836
Author(s):  
Shintaro Takase ◽  
Kouta Miyagawa ◽  
Hisafumi Ikeda
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Microelectrode Array ◽  
Pcr Amplification ◽  
Nucleic Acid Hybridization ◽  
Detection Sensitivity ◽  
Cyclic Voltammograms ◽  
Dna Duplexes ◽  
Label Free ◽  
Label Free Detection

To harness the applicability of microribonucleic acid (miRNA) as a cancer biomarker, the detection sensitivity of serum miRNA needs to be improved. This study evaluated the detection sensitivity of miRNA hybridization using cyclic voltammograms (CVs) and microelectrode array chips modified with peptide nucleic acid (PNA) probes and 6-hydroxy-1-hexanethiol. We investigated the PNA probe modification pattern on array chips using fluorescently labeled cDNA. The pattern was not uniformly spread over the working electrode (WE) and had a one-dimensional swirl-like pattern. Accordingly, we established a new ion-channel sensor model wherein the WE is negatively biased through the conductive π–π stacks of the PNA/DNA duplexes. This paper discusses the mechanism underlying the voltage shift in the CV curves based on the electric double-layer capacitance. Additionally, the novel hybridization evaluation parameter ΔE is introduced. Compared to conventional evaluation using oxidation current changes, ΔE was more sensitive. Using ΔE and a new hybridization system for ultrasmall amounts of aqueous solutions (as low as 35 pL), 140 zeptomol label-free miRNA were detected without polymerase chain reaction (PCR) amplification at an adequate sensitivity. Herein, the differences in the target molar amount and molar concentration are elucidated from the viewpoint of hybridization sensitivity.

scholarly journals Reduced Graphene Oxides: Influence of the Reduction Method on the Electrocatalytic Effect towards Nucleic Acid Oxidation

Nanomaterials ◽  
2017 ◽  
Vol 7 (7) ◽  
pp. 168 ◽  
Author(s):  
Daniela Báez ◽  
Helena Pardo ◽  
Ignacio Laborda ◽  
José Marco ◽  
Claudia Yáñez ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Reduction Method ◽  
Acid Oxidation ◽  
Graphene Oxides ◽  
Reduced Graphene ◽  
Electrocatalytic Effect ◽  
Reduced Graphene Oxides

Enhanced 1T phase promotes sodium storage performances of MoS2 flower-like spheres with embedded reduced graphene oxides

2021 ◽  
Vol 297 ◽  
pp. 122027
Author(s):  
Xianglin Yu ◽  
Ruixue Li ◽  
Xinyu Hu ◽  
Ren He ◽  
Kehui Xue ◽  
...  
Keyword(s):  
Graphene Oxides ◽  
Reduced Graphene ◽  
Reduced Graphene Oxides ◽  
Sodium Storage

scholarly journals Analysis of Reduced Graphene Oxides by X-ray Photoelectron Spectroscopy and Electrochemical Capacitance

2013 ◽  
Vol 42 (8) ◽  
pp. 924-926 ◽  
Author(s):  
Michio Koinuma ◽  
Hikaru Tateishi ◽  
Kazuto Hatakeyama ◽  
Shinsuke Miyamoto ◽  
Chikako Ogata ◽  
...  
Keyword(s):  
Photoelectron Spectroscopy ◽  
Graphene Oxides ◽  
Electrochemical Capacitance ◽  
X Ray ◽  
Reduced Graphene ◽  
Reduced Graphene Oxides

Effect of thermally reduced graphene oxides obtained at different temperatures on the barrier and mechanical properties of polypropylene/TRGO and polyamide‐6/TRGO nanocomposites

2018 ◽  
Vol 40 (S2) ◽  
pp. E1746-E1756 ◽  
Author(s):  
Moisés Gómez ◽  
Alexis Díaz ◽  
Paula Reyes ◽  
Mehrdad Yazdani‐Pedram ◽  
Sônia Marlí Bohrz Nachtigall ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Polyamide 6 ◽  
Graphene Oxides ◽  
Reduced Graphene ◽  
Different Temperatures ◽  
Reduced Graphene Oxides
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