In Vitro Assays Predictive of Telomerase Inhibitory Effect of G-Quadruplex Ligands in Cell Nuclei

2013 ◽  
Vol 118 (10) ◽  
pp. 2605-2614 ◽  
Author(s):  
Hidenobu Yaku ◽  
Takashi Murashima ◽  
Daisuke Miyoshi ◽  
Naoki Sugimoto
Keyword(s):  
Inhibitory Effect ◽  
In Vitro Assays ◽  
Cell Nuclei ◽  
G Quadruplex

Characterization of Serratia species and qualitative detection of Serratia marcescens in raw and pasteurized milk by an analytical method based on polymerase chain reaction

2021 ◽  
Vol 67 (2) ◽  
pp. 3453-3464
Author(s):  
Evelin Korcz ◽  
László Varga ◽  
Zoltán Kerényi
Keyword(s):  
Serratia Marcescens ◽  
Bacterial Species ◽  
Relevant Literature ◽  
Inhibitory Effect ◽  
Test Methods ◽  
In Vitro Assays ◽  
Pasteurized Milk ◽  
Milk Samples ◽  
Microbiological Methods

Serratia species are opportunistic pathogenic microorganisms primarily known as nosocomial infectious agents, which can also cause food quality problems. The appearance of the extracellular pigment-producing Serratia marcescens in cow’s milk causes its red discoloration, posing a challenge to the dairy industry and food certification laboratories. The detection of the bacterium by conventional procedures based on microbiological methods is time-consuming and labor-intensive, and in many cases does not lead to satisfactory results due to the competitive inhibitory effect of the accompanying microflora. Following the analysis of the relevant literature, the published endpoint PCR methods and the primers used for the detection of S. marcescens were evaluated in in silico and in vitro assays, and then the procedure was tested on farm milk samples. Using the method, a total of 60 raw and pasteurized milk samples were analyzed, more than half of which (i.e., 32) were identified as S. marcescens positive. The significance of our work is mainly represented by the application of the published test methods in food industry practice. Our results highlight to the importance of detecting this bacterial species.

scholarly journals Porphyra-334, a mycosporine-like amino acid, attenuates UV-induced apoptosis in HaCaT cells

2017 ◽  
Vol 67 (2) ◽  
pp. 257-264 ◽  
Author(s):  
Sung-Suk Suh ◽  
Se Kyung Oh ◽  
Sung Gu Lee ◽  
Il-Chan Kim ◽  
Sanghee Kim
Keyword(s):  
Caspase 3 ◽  
Biological Activities ◽  
Inhibitory Effect ◽  
In Vitro Assays ◽  
Control Group ◽  
Hacat Cells ◽  
Dramatic Decrease ◽  
Induced Apoptosis ◽  
Active Caspase

Abstract The main aim of the current research was to study the effect of porphyra-334, one of mycosporine-like amino acids (MAAs), well known as UV-absorbing compounds, on UVinduced apoptosis in human immortalized keratinocyte (HaCaT) cells. Due to their UV-screening capacity and ability to prevent UV-induced DNA damage, MAAs have recently attracted considerable attention in both industry and research in pharmacology. Herein, human HaCaT cells were used to determine the biological activities of porphyra- 334 by various in vitro assays, including proliferation, apoptosis and Western blot assays. The proliferation rate of UV-irradiated HaCaT cells was significantly decreased compared to the control group. Pretreatment with porphyra- 334 markedly attenuated the inhibitory effect of UV and induced a dramatic decrease in the apoptotic rate. Expression of active caspase-3 protein was increased in response to UV irradiation, while caspase-3 levels were similar between cells treated with porphyra-334 and the non-irradiated control group. Taken together, our data suggest that porphyra-334 inhibits UV-induced apoptosis in HaCaT cells through attenuation of the caspase pathway.

scholarly journals Potential Health Benefits of Origanum heracleoticum Essential Oil: Phytochemical and Biological Variability among Different Calabrian Populations

2018 ◽  
Vol 13 (9) ◽  
pp. 1934578X1801300 ◽  
Author(s):  
Mariangela Marrelli ◽  
Fabrizio Araniti ◽  
Maria Rosa Abenavoli ◽  
Giancarlo Statti ◽  
Filomena Conforti
Keyword(s):  
Essential Oils ◽  
Health Benefits ◽  
Hierarchical Cluster ◽  
Inhibitory Effect ◽  
Biological Properties ◽  
In Vitro Assays ◽  
No Production ◽  
Potential Health ◽  
Phytochemical Content

The potential health benefits of the essential oils of six different populations of Origanum heracleoticum L. from Calabria (Italy) were assessed, together with the evaluation of the influence of the site of collection on their metabolic profile and biological activity. Different terpenoids were identified, being thymol, carvacrol and o-cymene among the most abundant ones. Hierarchical cluster analysis allowed the identification of two main groups, which were discriminated by the altitude of collection. The antioxidant activity was tested by means of two in vitro assays: DPPH and β-carotene bleaching test. Samples EO-4 and EO-3 were the most active in protecting linoleic acid from peroxidation, with IC50 values of 4.00 and 4.68 μg/mL after 30 minutes of incubation. The anti-inflammatory potential was assessed through the evaluation of the capacity to inhibit NO production in lipopolysaccharide (LPS)-induced murine macrophage RAW 264.7 cell line. All essential oils induced a dose-depending inhibitory effect. An excellent activity was demonstrated for sample EO-4, followed by sample EO-3 (IC50 values equal to 32.77 and 49.48 μg/mL, respectively). Observed biological properties were correlated to the phytochemical content of analyzed samples.

scholarly journals Evaluation of cannabidiol’s inhibitory effect on alpha-glucosidase and its stability in simulated gastric and intestinal fluids

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Hang Ma ◽  
Huifang Li ◽  
Chang Liu ◽  
Navindra P. Seeram
Keyword(s):  
Molecular Docking ◽  
High Performance ◽  
Inhibitory Effect ◽  
In Vitro Assays ◽  
Inhibitory Effects ◽  
In Vivo Models ◽  
Glucosidase Activity ◽  
Intestinal Fluids

Abstract Objective Cannabidiol (CBD) has been reported to have anti-diabetic effects in pre-clinical and clinical studies but its inhibitory effects on α-glucosidase, a carbohydrate hydrolyzing enzyme, remain unknown. Herein, we evaluated CBD’s inhibitory effects on α-glucosidase using in vitro assays and computational studies. Methods CBD’s inhibitory effect on α-glucosidase activity was evaluated in a yeast enzymatic assay and by molecular docking. The stability of CBD in simulated gastric and intestinal fluids was evaluated by high-performance liquid chromatography analyses. Results CBD, at 10, 19, 38, 76, 152, 304, 608, and 1216 μM, inhibited α-glucosidase activity with inhibition of 17.1, 20.4, 48.1, 56.6, 59.1, 63.7, 74.1, and 95.4%, respectively. Acarbose, the positive control, showed a comparable inhibitory activity (with 85.1% inhibition at 608 μM). CBD’s inhibitory effect on α-glucosidase was supported by molecular docking showing binding energy (-6.39 kcal/mol) and interactions between CBD and the α-glucosidase protein. CBD was stable in simulated gastric and intestinal fluids for two hours (maintained ≥ 90.0%). Conclusions CBD showed moderate inhibitory effect against yeast α-glucosidase activity and was stable in gastric and intestinal fluids. However, further studies on CBD’s anti-α-glucosidase effects using cellular and in vivo models are warranted to support its potential application for the management of type II diabetes mellitus.

scholarly journals Colloidal Iron Oxide Formulation for Equine Hoof Disinfection

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 766
Author(s):  
Maurizio Isola ◽  
Cristina Piccinotti ◽  
Massimiliano Magro ◽  
Luca Fasolato ◽  
Fabio Vianello ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Colloidal Suspension ◽  
Bacterial Load ◽  
Inhibitory Effect ◽  
In Vitro Assays ◽  
Resistant Bacteria ◽  
Colloidal Iron ◽  
In Vivo Test

The presence of bacteria of various origins on horse hoofs enables the onset of infections following trauma or even post-surgical wounds. Thus, the analysis of new antibacterial substances is of fundamental importance. In this study, the antibacterial efficacy of Iron Animals (IA), a stable colloidal suspension of iron oxide, organic acids, and detergents, was tested in vitro and in vivo. In vitro assays were performed to test the unspecific inhibitory effect of IA on both gram-positive and gram-negative bacteria monitoring the microorganism growth by spectrophotometry (optical density OD600) at 37 °C for 24 h. In vivo test consists on the quantification of the bacterial load in colony forming units per gram (CFU/g) of specimens collected from the frog region of the anterior hooves of 11 horses. Sampling followed the application of four disinfectant protocols consisting of two consecutive 3 min scrubs with 50 mL of 10% Povidone-iodine (PI) or 4% Chlorhexidine (CHx), with or without an additional application for 15 min of 10 mL of Iron Animals (PI+IA and CHx+IA). In vitro, IA completely suppressed the bacterial growth of all the tested microorganisms, resulting in effectiveness also against CHx-resistant bacteria, such as Staphylococcus aureus. In vivo, PI emerged as an ineffective protocol; CHx was effective in 18% of cases, but with the addition of IA (CHx + IA) its use emerged as the best disinfectant protocol for horse hoof, achieving the lowest bacterial load in 55% of cases. The addition of IA, after PI or CHx, improves the effectiveness of both disinfectants leading to the highest bactericidal activity in 82% of cases.

α-Glucosidase and α-Amylase Inhibitory Effect and Antioxidant Activity of Aerial Part from Linaria aegyptiaca L.

2019 ◽  
Vol 15 (1) ◽  
pp. 22-27
Author(s):  
Abir Bekhaoua ◽  
Ihcen Khacheba ◽  
Hadjer Boussoussa ◽  
Mohamed Yousfi
Keyword(s):  
Antioxidant Activity ◽  
Aerial Part ◽  
Biological Effects ◽  
Antioxidant Properties ◽  
Inhibitory Effect ◽  
In Vitro Assays ◽  
Inhibitory Effects ◽  
Methanolic Extracts ◽  
Large Genus

Background: The genus Linaria belongs to the Scrophulariaceae family. It is a large genus comprising about 200 species. Various parts of several Linaria species have been reported to exhibit various biological effects. In Algeria especially in the Sahara and steppe regions, the different species of Linaria have several uses in dietary application. Objective: The aims of this study are to evaluate the α-Glucosidase and α-Amylase inhibitory effects and the antioxidant activity using in vitro assays by an organic extract of the aerial part of Linaria aegyptiaca collected in two months, April and June, from southern Algeria. Methods: The extracts were obtained with successful maceration in (hexane, dichloromethane, acetone and methanol). The phenolics and flavonoids contents of L. aegyptiaca extracts were evaluated with Folin- Ciocalteu and aluminum chloride reagents, respectively. Then, we studied their inhibitory effects on α-Glucosidase and α-Amylase enzymes. The antioxidant potential was determined in vitro with DPPH, ABT and Phosphomolybdate tests. Results: The highest phenolic and flavonoid content were detected in the methanolic extracts of Linaria aegyptiaca collected in April. All the extracts showed good inhibitory activity on both enzymes, where the best activity was against α- amylase by acetonic extract collected in June with an IC50 = 95.03 μg/ml. The evaluation of antioxidant activity showed that all the extracts exhibited a good antioxidant capacity compared to standard antioxidants. Conclusion: The aim of this research is to establish the anti-diabetic properties and the probable alpha glucosidase and alpha amylase inhibitory activities of Algerian Linaria aegyptiaca species. These results show that this species has good antioxidant properties and a good potential for hyperglycemia management, too. The Algerian Linaria aegyptiaca can be considered as a natural source of anti-hyperglycemic treatment and might be interesting for the prospect of new molecules with antidiabetic effect.

scholarly journals Biocontrol of Chickpea Fusarium Wilt by Bacillus Spp. Rhizobacteria

2013 ◽  
Vol 53 (2) ◽  
pp. 177-183 ◽  
Author(s):  
Souad Zaim ◽  
Lakhdar Belabid ◽  
Miloud Bellahcene
Keyword(s):  
Disease Control ◽  
Fusarium Wilt ◽  
Mycelial Growth ◽  
Field Trials ◽  
Inhibitory Effect ◽  
In Vitro Assays ◽  
Beneficial Effects ◽  
Volatile Metabolites ◽  
Bacillus Spp

Abstract Among 131 rhizobacteria isolates, 29 potentially antagonistic strains were screened in in vitro assays. The five antagonistic Bacillus spp. Rb29, Rb6, Rb12, Rb4, and Rb15 showed the most inhibitory effect against FOC1 (from 25.63 to 71.11%), mycelial growth, and FOC2 (from 28.43 to 60.65%) in vitro. Results also revealed that production of volatile metabolite, components and inhibition of the test pathogen by volatile metabolites varied among different antagonistic rhizobacteria. Isolates Rb29, Rb6, Rb12, Rb4, and Rb15 produced more volatile metabolites which inhibited mycelial FOC growth by 40%. Chickpea Fusarium wilt severity caused by FOC1 was reduced from 60 to 99% in the susceptible cultivar ILC 482 treated with antagonistic Bacillus spp. (Rb29, Rb6, Rb12, Rb4, and Rb15) in pot assays and by 98, 81, 68, 64, 57.20%, respectively, in the field trials. As for their beneficial effects on disease control, the results revealed that Bacillus spp. may improve plant growth and disease control.

scholarly journals A Short S-Equol Exposure Has a Long-Term Inhibitory Effect on Adipogenesis in Mouse 3T3-L1 Cells

2021 ◽  
Vol 11 (20) ◽  
pp. 9657
Author(s):  
Gilberto Mandujano-Lázaro ◽  
Carlos Galaviz-Hernández ◽  
César A. Reyes-López ◽  
Julio C. Almanza-Pérez ◽  
Abraham Giacoman-Martínez ◽  
...  
Keyword(s):  
Weight Control ◽  
Inhibitory Effect ◽  
New Drugs ◽  
Metabolic Effects ◽  
In Vitro Assays ◽  
Differentiation Protocol ◽  
Body Weight Control ◽  
The Impact

In the search for new drugs against obesity, the chronic disease that threatens human health worldwide, several works have focused on the study of estrogen homologs because of the role of estrogen receptors (ERs) in adipocyte growth. The isoflavone equol, an ERβ agonist, has shown beneficial metabolic effects in in vivo and in vitro assays; however, additional studies are required to better characterize its potential for body weight control. Here, we showed that the treatment of 3T3-L1 cells with 10 μM of S-equol for the first three days of the adipocyte differentiation protocol was able to prevent cells becoming semi-rounded and having a lipid droplet formation until the seventh day of culture; moreover, lipid accumulation was reduced by about 50%. Congruently, S-equol induced a reduction in mRNA expression of the adipogenic markers C/EBPα and PPARγ, and adipokines secretion, mainly Adiponectin, Leptin, Resistin, and MCP-1, while the release of PAI-1 was augmented. Moreover, it also reduced the expression of ERα and attenuated the subexpression of ERβ associated with adipogenesis. Altogether, our data suggested that S-equol binding to ERβ affects the transcriptional program that regulates adipogenesis and alters adipocyte functions. Future efforts will focus on studying the impact of S-equol on ER signaling pathways.

scholarly journals Anti-Trypanosoma cruzi Activity of Metabolism Modifier Compounds

2021 ◽  
Vol 22 (2) ◽  
pp. 688 ◽  
Author(s):  
Nieves Martinez-Peinado ◽  
Clara Martori ◽  
Nuria Cortes-Serra ◽  
Julian Sherman ◽  
Ana Rodriguez ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Developmental Stages ◽  
Cell Metabolism ◽  
Heat Shock Protein 90 ◽  
Inhibitory Effect ◽  
Parasite Growth ◽  
New Drugs ◽  
In Vitro Assays ◽  
Growth Environment

Chagas disease is caused by the protozoan parasite Trypanosoma cruzi and affects over 6 million people worldwide. Development of new drugs to treat this disease remains a priority since those currently available have variable efficacy and frequent adverse effects, especially during the long regimens required for treating the chronic stage of the disease. T. cruzi modulates the host cell-metabolism to accommodate the cell cytosol into a favorable growth environment and acquire nutrients for its multiplication. In this study we evaluated the specific anti-T. cruzi activity of nine bio-energetic modulator compounds. Notably, we identified that 17-DMAG, which targets the ATP-binding site of heat shock protein 90 (Hsp90), has a very high (sub-micromolar range) selective inhibition of the parasite growth. This inhibitory effect was also highly potent (IC50 = 0.27 μmol L−1) against the amastigote intracellular replicative stage of the parasite. Moreover, molecular docking results suggest that 17-DMAG may bind T. cruzi Hsp90 homologue Hsp83 with good affinity. Evaluation in a mouse model of chronic T. cruzi infection did not show parasite growth inhibition, highlighting the difficulties encountered when going from in vitro assays onto preclinical drug developmental stages.

scholarly journals Antrodia cinnamomea Enhances Chemo-Sensitivity of 5-FU and Suppresses Colon Tumorigenesis and Cancer Stemness via Up-Regulation of Tumor Suppressor miR-142-3p

Biomolecules ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 306 ◽  
Author(s):  
Yan-Jiun Huang ◽  
Vijesh Kumar Yadav ◽  
Prateeti Srivastava ◽  
Alexander TH Wu ◽  
Thanh-Tuan Huynh ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Tumor Suppressor ◽  
Colony Formation ◽  
Inhibitory Effect ◽  
In Vitro Assays ◽  
Cancer Stemness ◽  
Antrodia Cinnamomea ◽  
Tumor Sphere

5-Fluorouracil (5-FU) regimen remains the backbone of the first-line agent to treat colon cancer, but often these patients develop resistance. Cancer stem cells (CSC’s) are considered as one of the key contributors in the development of drug resistance and tumor recurrence. We aimed to provide preclinical evidence for Antrodia cinnamomea (AC), as a potential in suppressing colon cancer CSC’s to overcome 5-FU drug-resistant. In-vitro assays including cell viability, colony formation, AC + 5-FU drug combination index and tumor sphere generation were applied to determine the inhibitory effect of AC. Mouse xenograft models also incorporated to evaluate in vivo effect of AC. AC treatment significantly inhibited the proliferation, colony formation and tumor sphere generation. AC also inhibited the expression of oncogenic markers (NF-κB, and C-myc), EMT/metastasis markers (vimentin and MMP3) and stemness associated markers (β-catenin, SOX-2 and Nanog). Sequential treatment of AC and 5-FU synergized and reduces colon cancer viability both in vivo and in vitro. Mechanistically, AC mediated anti-tumor effect was associated with an increased level of tumor suppressor microRNAs especially, miR142-3p. AC can be a potent synergistic adjuvant, down-regulates cancer stemness genes and enhances the antitumor ability of 5-FU by stimulating apoptosis-associated genes, suppressing inflammation and metastasis genes through miR142-3p in colon cancer.

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