Detailed Study of DNA Hairpin Dynamics Using Single-Molecule Fluorescence Assisted by DNA Origami

2013 ◽  
Vol 117 (40) ◽  
pp. 11932-11942 ◽  
Author(s):  
Roman Tsukanov ◽  
Toma E. Tomov ◽  
Rula Masoud ◽  
Hagai Drory ◽  
Noa Plavner ◽  
...  
Keyword(s):  
Single Molecule ◽  
Dna Origami ◽  
Dna Hairpin ◽  
Single Molecule Fluorescence

scholarly journals Human T-Cell Lymphotropic Virus Type 1 Nucleocapsid Protein-Induced Structural Changes in Transactivation Response DNA Hairpin Measured by Single-Molecule Fluorescence Resonance Energy Transfer

2008 ◽  
Vol 82 (24) ◽  
pp. 12164-12171 ◽  
Author(s):  
Qusai Darugar ◽  
Hannah Kim ◽  
Robert J. Gorelick ◽  
Christy Landes
Keyword(s):  
Energy Transfer ◽  
Virus Type ◽  
Single Molecule ◽  
Nucleocapsid Protein ◽  
Structural Changes ◽  
Dna Hairpin ◽  
Single Molecule Fluorescence ◽  
Human T Cell ◽  
Hiv 1

ABSTRACT Time-resolved single-molecule fluorescence spectroscopy was used to study the human T-cell lymphotropic virus type 1 (HTLV-1) nucleocapsid protein (NC) chaperone activity compared to that of the human immunodeficiency virus type 1 (HIV-1) NC protein. HTLV-1 NC contains two zinc fingers, each having a CCHC binding motif similar to HIV-1 NC. HIV-1 NC is required for recognition and packaging of the viral RNA and is also a nucleic acid chaperone protein that facilitates nucleic acid restructuring during reverse transcription. Because of similarities in structures between the two retroviruses, we have used single-molecule fluorescence energy transfer to investigate the chaperoning activity of the HTLV-1 NC protein. The results indicate that the HTLV-1 NC protein induces structural changes by opening the transactivation response (TAR) DNA hairpin to an even greater extent than HIV-1 NC. However, unlike HIV-1 NC, HTLV-1 NC does not chaperone the strand-transfer reaction involving TAR DNA. These results suggest that, despite its effective destabilization capability, HTLV-1 NC is not as effective at overall chaperone function as is its HIV-1 counterpart.

scholarly journals Single-Molecule Fluorescence Meets DNA Origami

2012 ◽  
Vol 102 (3) ◽  
pp. 388a
Author(s):  
Ingo H. Stein ◽  
Christian Steinhauer ◽  
Verena Schüller ◽  
Tim Liedl ◽  
Philip Tinnefeld
Keyword(s):  
Single Molecule ◽  
Dna Origami ◽  
Single Molecule Fluorescence

Combining gold nanoparticle antennas with single-molecule fluorescence resonance energy transfer (smFRET) to study DNA hairpin dynamics

Nanoscale ◽  
2018 ◽  
Vol 10 (14) ◽  
pp. 6611-6619 ◽  
Author(s):  
Jinyong Hu ◽  
Meiyan Wu ◽  
Li Jiang ◽  
Zhensheng Zhong ◽  
Zhangkai Zhou ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Gold Nanoparticle ◽  
Fluorescence Resonance Energy Transfer ◽  
Single Molecule ◽  
Fluorescence Enhancement ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Dna Hairpin ◽  
Single Molecule Fluorescence ◽  
Fluorescence Resonance

Gold nanoparticle antennas as a promising platform not only for fluorescence enhancement but also for the studies of single-molecule kinetics.

Angular modulation of single-molecule fluorescence by gold nanoparticles on DNA origami templates

Nanophotonics ◽  
2013 ◽  
Vol 2 (3) ◽  
pp. 167-172 ◽  
Author(s):  
Friederike M. Möller ◽  
Phil Holzmeister ◽  
Tapasi Sen ◽  
Guillermo P. Acuna ◽  
Philip Tinnefeld
Keyword(s):  
Single Molecule ◽  
Fluorescence Intensity ◽  
Three Dimensional ◽  
Dna Origami ◽  
Single Molecule Fluorescence ◽  
Excitation Light ◽  
Fixed Distance ◽  
Linearly Polarized ◽  
Intensity Map ◽  
Good Agreement

AbstractWe study the angular fluorescence intensity modulation of a single dye positioned near a spherical gold nanoparticle, induced by rotation of linearly polarized excitation light. Accurate positioning and alignment of nanoparticle and fluorophore with respect to each other and the incoming electric field is achieved by a three-dimensional, self-assembled DNA origami. An intensity map is obtained for a fixed distance and two different nanoparticle diameters, revealing polarization-dependent enhancement and quenching of fluorescence intensity in good agreement to numerical simulations.

Angular modulation of single-molecule fluorescence by gold nanoparticles on DNA origami templates

2019 ◽  
Author(s):  
Friederike M. Möller ◽  
Phil Holzmeister ◽  
Tapasi Sen ◽  
Guillermo P. Acuna ◽  
Philip Tinnefeld
Keyword(s):  
Gold Nanoparticles ◽  
Single Molecule ◽  
Dna Origami ◽  
Single Molecule Fluorescence

scholarly journals Single Molecule Fluorescence Studies of Transition Paths in DNA Hairpin Folding

2016 ◽  
Vol 110 (3) ◽  
pp. 635a
Author(s):  
Katherine Truex ◽  
Hoi Sung Chung ◽  
John M. Louis ◽  
William A. Eaton
Keyword(s):  
Single Molecule ◽  
Dna Hairpin ◽  
Single Molecule Fluorescence ◽  
Fluorescence Studies ◽  
Transition Paths
Sign in / Sign up

Export Citation Format

Share Document