Accelerating the Phase Separation in Aqueous Poly(N-isopropylacrylamide) Solutions by Slight Modification of the Polymer Stereoregularity: A Single Molecule Fluorescence Study

2013 ◽  
Vol 117 (20) ◽  
pp. 10818-10824 ◽  
Author(s):  
Takanori Tada ◽  
Yukiteru Katsumoto ◽  
Karel Goossens ◽  
Hiroshi Uji-i ◽  
Johan Hofkens ◽  
...  
Keyword(s):  
Phase Separation ◽  
Single Molecule ◽  
Slight Modification ◽  
Single Molecule Fluorescence ◽  
Fluorescence Study

scholarly journals Fast Biosynthesis of GFP Molecules - A Single Molecule Fluorescence Study

2010 ◽  
Vol 98 (3) ◽  
pp. 259a-260a
Author(s):  
Georg Büldt ◽  
Alexandros Katranidis ◽  
Ramona Schlesinger ◽  
Knud H. Nierhaus ◽  
Ingo Gregor ◽  
...  
Keyword(s):  
Single Molecule ◽  
Single Molecule Fluorescence ◽  
Fluorescence Study

scholarly journals Single-Molecule Fluorescence Study of RNA Recognition by Viral RNAi Suppressors

2017 ◽  
Vol 112 (3) ◽  
pp. 151a
Author(s):  
Mohamed Fareh ◽  
Jasper van Lopik ◽  
Iason Katechis ◽  
Ronald van Rij ◽  
Chirlmin Joo
Keyword(s):  
Single Molecule ◽  
Rna Recognition ◽  
Single Molecule Fluorescence ◽  
Fluorescence Study

scholarly journals Single Molecule Fluorescence Study of G-Quadruplex Sensor

2015 ◽  
Vol 108 (2) ◽  
pp. 164a
Author(s):  
Yingya Li ◽  
Xiao Fan ◽  
Yanyan Li ◽  
Haitao Li
Keyword(s):  
Single Molecule ◽  
Single Molecule Fluorescence ◽  
Fluorescence Study ◽  
G Quadruplex

scholarly journals Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA

2019 ◽  
Author(s):  
Wooyoung Kang ◽  
Kook Sun Ha ◽  
Heesoo Uhm ◽  
Kyuhyong Park ◽  
Ja Yil Lee ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Single Molecule ◽  
Sigma Factor ◽  
Single Molecule Fluorescence ◽  
Fluorescence Study ◽  
Transcription Reinitiation ◽  
Dna Template ◽  
Rna Transcript

(Abstract)Despite extensive studies on transcription mechanisms, it is unknown how termination complexes are disassembled, especially in what order the components dissociate. Our single-molecule fluorescence study unveils that RNA transcript release precedes RNA polymerase (RNAP) dissociation from DNA template in bacterial intrinsic termination of transcription much more often than concurrent dissociation. As termination is defined by release of product RNA from transcription complex, the subsequent retention of RNAP on DNA constitutes a previously unidentified stage, termed here as ‘recycling.’ During the recycling stage, RNAPs one-dimensionally diffuse on DNA in downward and upward directions, and these RNAPs can initiate transcription again at nearby promoters in case of retaining a sigma factor. The efficiency of this event, termed here as ‘reinitiation,’ increases with supplement of a sigma factor. In summary, after releasing RNA product at intrinsic termination, recycling RNAP diffuses on DNA template for reinitiation most times.

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