Single-Molecule FRET Studies of RNA Folding: A Diels–Alderase Ribozyme with Photolabile Nucleotide Modifications

2013 ◽  
Vol 117 (42) ◽  
pp. 12800-12806 ◽  
Author(s):  
Andrei Yu. Kobitski ◽  
Stefan Schäfer ◽  
Alexander Nierth ◽  
Marco Singer ◽  
Andres Jäschke ◽  
...  
Keyword(s):  
Single Molecule ◽  
Rna Folding ◽  
Single Molecule Fret

scholarly journals Complex RNA Folding Kinetics Revealed by Single-Molecule FRET and Hidden Markov Models

2014 ◽  
Vol 136 (12) ◽  
pp. 4534-4543 ◽  
Author(s):  
Bettina G. Keller ◽  
Andrei Kobitski ◽  
Andres Jäschke ◽  
G. Ulrich Nienhaus ◽  
Frank Noé
Keyword(s):  
Hidden Markov Models ◽  
Single Molecule ◽  
Rna Folding ◽  
Markov Models ◽  
Hidden Markov ◽  
Folding Kinetics ◽  
Single Molecule Fret

scholarly journals Quantitative tests of a reconstitution model for RNA folding thermodynamics and kinetics

2017 ◽  
Vol 114 (37) ◽  
pp. E7688-E7696 ◽  
Author(s):  
Namita Bisaria ◽  
Max Greenfeld ◽  
Charles Limouse ◽  
Hideo Mabuchi ◽  
Daniel Herschlag
Keyword(s):  
Single Molecule ◽  
Rna Folding ◽  
Tertiary Structure ◽  
Additive Model ◽  
Thermodynamics And Kinetics ◽  
Single Molecule Fret ◽  
Tertiary Contacts ◽  
Predictive Understanding ◽  
The Individual ◽  
Folding Thermodynamics

Decades of study of the architecture and function of structured RNAs have led to the perspective that RNA tertiary structure is modular, made of locally stable domains that retain their structure across RNAs. We formalize a hypothesis inspired by this modularity—that RNA folding thermodynamics and kinetics can be quantitatively predicted from separable energetic contributions of the individual components of a complex RNA. This reconstitution hypothesis considers RNA tertiary folding in terms of ΔGalign, the probability of aligning tertiary contact partners, and ΔGtert, the favorable energetic contribution from the formation of tertiary contacts in an aligned state. This hypothesis predicts that changes in the alignment of tertiary contacts from different connecting helices and junctions (ΔGHJH) or from changes in the electrostatic environment (ΔG+/−) will not affect the energetic perturbation from a mutation in a tertiary contact (ΔΔGtert). Consistent with these predictions, single-molecule FRET measurements of folding of model RNAs revealed constant ΔΔGtert values for mutations in a tertiary contact embedded in different structural contexts and under different electrostatic conditions. The kinetic effects of these mutations provide further support for modular behavior of RNA elements and suggest that tertiary mutations may be used to identify rate-limiting steps and dissect folding and assembly pathways for complex RNAs. Overall, our model and results are foundational for a predictive understanding of RNA folding that will allow manipulation of RNA folding thermodynamics and kinetics. Conversely, the approaches herein can identify cases where an independent, additive model cannot be applied and so require additional investigation.

scholarly journals Encoding Multiple Virtual Signals in DNA Barcodes with Single-Molecule FRET

Nano Letters ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1694-1701 ◽  
Author(s):  
Sung Hyun Kim ◽  
Hyunwoo Kim ◽  
Hawoong Jeong ◽  
Tae-Young Yoon
Keyword(s):  
Single Molecule ◽  
Dna Barcodes ◽  
Single Molecule Fret

Single-Molecule FRET-Based Dynamic DNA Sensor

ACS Sensors ◽  
2021 ◽  
Author(s):  
Anoja Megalathan ◽  
Kalani M. Wijesinghe ◽  
Soma Dhakal
Keyword(s):  
Single Molecule ◽  
Dna Sensor ◽  
Single Molecule Fret
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