scholarly journals Thioflavine-T and Congo Red Reveal the Polymorphism of Insulin Amyloid Fibrils When Probed by Polarization-Resolved Fluorescence Microscopy

2013 ◽  
Vol 117 (3) ◽  
pp. 784-788 ◽  
Author(s):  
Julien Duboisset ◽  
Patrick Ferrand ◽  
Wei He ◽  
Xiao Wang ◽  
Hervé Rigneault ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Congo Red ◽  
Amyloid Fibrils ◽  
Thioflavine T

scholarly journals Fluorescence detection of amyloid deposits in human tissues using histochemical dyes

2021 ◽  
Author(s):  
VV Guselnikova ◽  
DA Sufieva ◽  
DL Tsyba ◽  
DE Korzhevskii
Keyword(s):  
Fluorescence Microscopy ◽  
Fluorescence Detection ◽  
Congo Red ◽  
Amyloid Fibrils ◽  
Polarized Light ◽  
Methyl Violet ◽  
Human Tissues ◽  
Transthyretin Amyloidosis ◽  
Amyloid Deposits ◽  
Β Amyloid

Recently, fluorescence microscopy becomes more available, presenting new opportunities to face several challenges of experimental biology and medicine. The study was aimed to assess the effectiveness of fluorescence microscopy for the identification of amyloid deposits in human tissues. Post-mortem samples of the myocardium (n = 12) and cerebral cortex (n = 8) obtained from subjects of both sexes aged 60–98 with verified amyloidosis were used as a material for the study. The specimens were stained using 11 different histochemical dyes and subsequently analyzed by light and fluorescence microscopy. Qualitative and quantitative analysis has shown that Thioflavin T is the most effective stain for fluorescence detection of β- and transthyretin amyloid in human tissues. Congo red staining is highly effective for the detection of transthyretin amyloidosis, however, it is ill-suited for the identification of β-amyloid plaques. It has been found that the ability of Congo red to exhibit fluorescence when binding to amyloid fibrils can be used for verification of amyloid deposits instead of the traditional polarized light microscopy. As has been first noted, methyl violet can selectively bind to β-amyloid with fluorescent complex formation. In addition, methyl violet treatment effectively reduces the autofluorescent background in the nervous tissue. This makes methyl violet staining a promising diagnostic tool for Alzheimer's-type pathology.

scholarly journals Intracellular Aluminium in Inflammatory and Glial Cells in Cerebral Amyloid Angiopathy: A Case Report

2019 ◽  
Vol 16 (8) ◽  
pp. 1459 ◽  
Author(s):  
Mold ◽  
Cottle ◽  
King ◽  
Exley
Keyword(s):  
Fluorescence Microscopy ◽  
Brain Tissue ◽  
Glial Cells ◽  
Cerebral Amyloid Angiopathy ◽  
Congo Red ◽  
Unusual Case ◽  
Amyloid Angiopathy ◽  
Specific Fluorescence ◽  
Cerebral Amyloid ◽  
The Individual

(1) Introduction: In 2006, we reported on very high levels of aluminium in brain tissue in an unusual case of cerebral amyloid angiopathy (CAA). The individual concerned had been exposed to extremely high levels of aluminium in their potable water due to a notorious pollution incident in Camelford, Cornwall, in the United Kingdom. The recent development of aluminium-specific fluorescence microscopy has now allowed for the location of aluminium in this brain to be identified. (2) Case Summary: We used aluminium-specific fluorescence microscopy in parallel with Congo red staining and polarised light to identify the location of aluminium and amyloid in brain tissue from an individual who had died from a rare and unusual case of CAA. Aluminium was almost exclusively intracellular and predominantly in inflammatory and glial cells including microglia, astrocytes, lymphocytes and cells lining the choroid plexus. Complementary staining with Congo red demonstrated that aluminium and amyloid were not co-located in these tissues. (3) Discussion: The observation of predominantly intracellular aluminium in these tissues was novel and something similar has only previously been observed in cases of autism. The results suggest a strong inflammatory component in this case and support a role for aluminium in this rare and unusual case of CAA.

Fluorometric determination of amyloid fibrils in vitro using the fluorescent dye, thioflavine T

1989 ◽  
Vol 177 (2) ◽  
pp. 244-249 ◽  
Author(s):  
Hironobu Naiki ◽  
Keiichi Higuchi ◽  
Masanori Hosokawa ◽  
Toshio Takeda
Keyword(s):  
Amyloid Fibrils ◽  
Fluorescent Dye ◽  
Fluorometric Determination ◽  
Thioflavine T

scholarly journals CONGO RED DICHROISM WITH DISPERSED AMYLOID FIBRILS, AN EXTRINSIC COTTON EFFECT

1970 ◽  
Vol 66 (4) ◽  
pp. 1044-1051 ◽  
Author(s):  
E. P. Benditt ◽  
N. Eriksen ◽  
C. Berglund
Keyword(s):  
Congo Red ◽  
Amyloid Fibrils ◽  
Cotton Effect

scholarly journals The conformation of the Congo-red ligand bound to amyloid fibrils HET-s(218–289): a solid-state NMR study

2017 ◽  
Vol 69 (4) ◽  
pp. 207-213 ◽  
Author(s):  
Chandrakala Gowda ◽  
Giorgia Zandomeneghi ◽  
Herbert Zimmermann ◽  
Anne K. Schütz ◽  
Anja Böckmann ◽  
...  
Keyword(s):  
Solid State ◽  
Congo Red ◽  
Solid State Nmr ◽  
Amyloid Fibrils ◽  
Nmr Study

Fluorometric Examination of Tissue Amyloid Fibrils in Murine Senile Amyloidosis: Use of the Fluorescent Indicator, Thioflavine T

1991 ◽  
pp. 393-396 ◽  
Author(s):  
Hironobu Naiki ◽  
Keiichi Higuchi ◽  
Kaori Kitagawa ◽  
Atsuyoshi Shimada ◽  
When-Hsi Chen ◽  
...  
Keyword(s):  
Amyloid Fibrils ◽  
Fluorescent Indicator ◽  
Thioflavine T
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