The Central Role of Gln63 for the Hydrogen Bonding Network and UV–Visible Spectrum of the AppA BLUF Domain

2012 ◽  
Vol 116 (28) ◽  
pp. 8064-8073 ◽  
Author(s):  
Ya-Wen Hsiao ◽  
Jan P. Götze ◽  
Walter Thiel
Keyword(s):  
Hydrogen Bonding ◽  
Visible Spectrum ◽  
Uv Visible ◽  
Bluf Domain ◽  
Hydrogen Bonding Network

Liquid-liquid extraction of succinate using a dicopper cryptate

2020 ◽  
Author(s):  
Riccardo Mobili ◽  
Sonia La Cognata ◽  
Francesca Merlo ◽  
Andrea Speltini ◽  
Massimo Boiocchi ◽  
...  
Keyword(s):  
Aqueous Phase ◽  
Visible Spectrum ◽  
Tca Cycle ◽  
Residual Concentration ◽  
Waste Products ◽  
Liquid Liquid Extraction ◽  
The Tca Cycle ◽  
Quantitative Extraction ◽  
Uv Visible

<div> <p>The extraction of the succinate dianion from a neutral aqueous solution into dichloromethane is obtained using a lipophilic cage-like dicopper(II) complex as the extractant. The quantitative extraction exploits the high affinity of the succinate anion for the cavity of the azacryptate. The anion is effectively transferred from the aqueous phase, buffered at pH 7 with HEPES, into dichloromethane. A 1:1 extractant:anion adduct is obtained. Extraction can be easily monitored by following changes in the UV-visible spectrum of the dicopper complex in dichloromethane, and by measuring the residual concentration of succinate in the aqueous phase by HPLC−UV. Considering i) the relevance of polycarboxylates in biochemistry, as e.g. normal intermediates of the TCA cycle, ii) the relevance of dicarboxylates in the environmental field, as e.g. waste products of industrial processes, and iii) the recently discovered role of succinate and other dicarboxylates in pathophysiological processes including cancer, our results open new perspectives for research in all contexts where selective recognition, trapping and extraction of polycarboxylates is required. </p> </div>

scholarly journals Liquid-liquid extraction of succinate using a dicopper cryptate

2020 ◽  
Author(s):  
Riccardo Mobili ◽  
Sonia La Cognata ◽  
Francesca Merlo ◽  
Andrea Speltini ◽  
Massimo Boiocchi ◽  
...  
Keyword(s):  
Aqueous Phase ◽  
Visible Spectrum ◽  
Tca Cycle ◽  
Residual Concentration ◽  
Waste Products ◽  
Liquid Liquid Extraction ◽  
The Tca Cycle ◽  
Quantitative Extraction ◽  
Uv Visible

<div> <p>The extraction of the succinate dianion from a neutral aqueous solution into dichloromethane is obtained using a lipophilic cage-like dicopper(II) complex as the extractant. The quantitative extraction exploits the high affinity of the succinate anion for the cavity of the azacryptate. The anion is effectively transferred from the aqueous phase, buffered at pH 7 with HEPES, into dichloromethane. A 1:1 extractant:anion adduct is obtained. Extraction can be easily monitored by following changes in the UV-visible spectrum of the dicopper complex in dichloromethane, and by measuring the residual concentration of succinate in the aqueous phase by HPLC−UV. Considering i) the relevance of polycarboxylates in biochemistry, as e.g. normal intermediates of the TCA cycle, ii) the relevance of dicarboxylates in the environmental field, as e.g. waste products of industrial processes, and iii) the recently discovered role of succinate and other dicarboxylates in pathophysiological processes including cancer, our results open new perspectives for research in all contexts where selective recognition, trapping and extraction of polycarboxylates is required. </p> </div>

Model Complexes Elucidate the Role of the Proximal Hydrogen-Bonding Network in Cytochrome P450s

2020 ◽  
Vol 59 (12) ◽  
pp. 8034-8043
Author(s):  
Andrew P. Hunt ◽  
Subhra Samanta ◽  
Matthew R. Dent ◽  
Michael W. Milbauer ◽  
Judith N. Burstyn ◽  
...  
Keyword(s):  
Hydrogen Bonding ◽  
Cytochrome P450s ◽  
Model Complexes ◽  
Hydrogen Bonding Network

Investigation into the role of the hydrogen bonding network in cyclodextrin-based self-assembling mesophases

2014 ◽  
Vol 2 (25) ◽  
pp. 4928-4936 ◽  
Author(s):  
Sandra Ward ◽  
Oliver Calderon ◽  
Ping Zhang ◽  
Matthew Sobchuk ◽  
Samantha N. Keller ◽  
...  
Keyword(s):  
Hydrogen Bonding ◽  
Self Organized ◽  
Self Assembling ◽  
Bond Network ◽  
Hydrogen Bonding Network

The ability to form self-organized thermotropic mesophases of amphiphilic cyclodextrins correlates well with their ability to establish an intermolecular H-bond network.

scholarly journals Molecular Dynamics Study of Twister Ribozyme: Role of Mg2+Ions and the Hydrogen-Bonding Network in the Active Site

Biochemistry ◽  
2016 ◽  
Vol 55 (27) ◽  
pp. 3834-3846 ◽  
Author(s):  
Melek N. Ucisik ◽  
Philip C. Bevilacqua ◽  
Sharon Hammes-Schiffer
Keyword(s):  
Molecular Dynamics ◽  
Hydrogen Bonding ◽  
Active Site ◽  
Hydrogen Bonding Network
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