SRLS Analysis of 15N Spin Relaxation from E. coli Ribonuclease HI: The Tensorial Perspective

2012 ◽  
Vol 116 (2) ◽  
pp. 886-894 ◽  
Author(s):  
Eva Meirovitch ◽  
Yury E. Shapiro ◽  
Mirco Zerbetto ◽  
Antonino Polimeno
Keyword(s):  
Spin Relaxation ◽  
E Coli ◽  
15N Spin Relaxation

Practical Aspects of Two-Dimensional Proton-Detected 15N Spin Relaxation Measurements

1993 ◽  
Vol 102 (3) ◽  
pp. 253-264 ◽  
Author(s):  
N.J. Skelton ◽  
A.G. Palmer ◽  
M. Akke ◽  
J. Kordel ◽  
M. Rance ◽  
...  
Keyword(s):  
Spin Relaxation ◽  
Two Dimensional ◽  
Relaxation Measurements ◽  
15N Spin Relaxation

scholarly journals What Drives 15N Spin Relaxation in Disordered Proteins? Combined NMR/MD Study of the H4 Histone Tail

2018 ◽  
Vol 115 (12) ◽  
pp. 2348-2367 ◽  
Author(s):  
Kerstin Kämpf ◽  
Sergei A. Izmailov ◽  
Sevastyan O. Rabdano ◽  
Adam T. Groves ◽  
Ivan S. Podkorytov ◽  
...  
Keyword(s):  
Spin Relaxation ◽  
Disordered Proteins ◽  
Histone Tail ◽  
15N Spin Relaxation

Protein dynamics studied by rotating frame 15N spin relaxation times

1993 ◽  
Vol 3 (2) ◽  
Author(s):  
T. Szyperski ◽  
P. Luginb�hl ◽  
G. Otting ◽  
P. G�ntert ◽  
K. W�thrich
Keyword(s):  
Protein Dynamics ◽  
Spin Relaxation ◽  
Relaxation Times ◽  
Rotating Frame ◽  
15N Spin Relaxation

Endotoxin Alteration of the Mucopolysaccharide Blood Vessel Coating in Rats

1974 ◽  
Vol 32 ◽  
pp. 148-149
Author(s):  
D. E. Philpott ◽  
A. Takahashi
Keyword(s):  
Skeletal Muscle ◽  
Endothelial Cells ◽  
Salivary Gland ◽  
Carotid Artery ◽  
Basement Membrane ◽  
Coronary Vessels ◽  
Ruthenium Red ◽  
E Coli ◽  
Old Rats ◽  
The Brain

Two month, eight month and two year old rats were treated with 10 or 20 mg/kg of E. Coli endotoxin I. P. The eight month old rats proved most resistant to the endotoxin. During fixation the aorta, carotid artery, basil arartery of the brain, coronary vessels of the heart, inner surfaces of the heart chambers, heart and skeletal muscle, lung, liver, kidney, spleen, brain, retina, trachae, intestine, salivary gland, adrenal gland and gingiva were treated with ruthenium red or alcian blue to preserve the mucopolysaccharide (MPS) coating. Five, 8 and 24 hrs of endotoxin treatment produced increasingly marked capillary damage, disappearance of the MPS coating, edema, destruction of endothelial cells and damage to the basement membrane in the liver, kidney and lung.

Ribosome Structural Organization

1974 ◽  
Vol 32 ◽  
pp. 332-333
Author(s):  
James A. Lake
Keyword(s):  
Ribosomal Proteins ◽  
Structural Organization ◽  
Three Dimensional ◽  
Small Subunit ◽  
Structural Studies ◽  
X Ray Diffraction ◽  
Specific Antibodies ◽  
X Ray ◽  
E Coli ◽  
Complete Sequences

The understanding of ribosome structure has advanced considerably in the last several years. Biochemists have characterized the constituent proteins and rRNA's of ribosomes. Complete sequences have been determined for some ribosomal proteins and specific antibodies have been prepared against all E. coli small subunit proteins. In addition, a number of naturally occuring systems of three dimensional ribosome crystals which are suitable for structural studies have been observed in eukaryotes. Although the crystals are, in general, too small for X-ray diffraction, their size is ideal for electron microscopy.

Sites of Adsorption of the Bacteriophages T3 and T5 on the Wall of Escherichia Coli B.

1967 ◽  
Vol 25 ◽  
pp. 96-97
Author(s):  
Manfred E. Bayer
Keyword(s):  
Escherichia Coli ◽  
Cell Wall ◽  
Electron Microscope ◽  
Uranyl Acetate ◽  
E Coli ◽  
Receptor Sites ◽  
Rigid Cell Wall ◽  
Host Cell Surface ◽  
Bacterial Viruses ◽  
Escherichia Coli B

Bacterial viruses adsorb specifically to receptors on the host cell surface. Although the chemical composition of some of the cell wall receptors for bacteriophages of the T-series has been described and the number of receptor sites has been estimated to be 150 to 300 per E. coli cell, the localization of the sites on the bacterial wall has been unknown.When logarithmically growing cells of E. coli are transferred into a medium containing 20% sucrose, the cells plasmolize: the protoplast shrinks and becomes separated from the somewhat rigid cell wall. When these cells are fixed in 8% Formaldehyde, post-fixed in OsO4/uranyl acetate, embedded in Vestopal W, then cut in an ultramicrotome and observed with the electron microscope, the separation of protoplast and wall becomes clearly visible, (Fig. 1, 2). At a number of locations however, the protoplasmic membrane adheres to the wall even under the considerable pull of the shrinking protoplast. Thus numerous connecting bridges are maintained between protoplast and cell wall. Estimations of the total number of such wall/membrane associations yield a number of about 300 per cell.

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