Comment on “A Sulfonium Cation Intermediate in the Mechanism of Methionine Sulfoxide Reductase B: A DFT Study”

2011 ◽  
Vol 115 (36) ◽  
pp. 10775-10775 ◽  
Author(s):  
Fabrice Neiers ◽  
Sandrine Boschi-Muller ◽  
Guy Branlant
Keyword(s):  
Methionine Sulfoxide ◽  
Methionine Sulfoxide Reductase ◽  
Dft Study ◽  
Methionine Sulfoxide Reductase B

A Sulfonium Cation Intermediate in the Mechanism of Methionine Sulfoxide Reductase B: A DFT Study

2011 ◽  
Vol 115 (29) ◽  
pp. 9202-9212 ◽  
Author(s):  
Jesse J. Robinet ◽  
Hisham. M. Dokainish ◽  
David J. Paterson ◽  
James W. Gauld
Keyword(s):  
Methionine Sulfoxide ◽  
Methionine Sulfoxide Reductase ◽  
Dft Study ◽  
Methionine Sulfoxide Reductase B

scholarly journals Contribution of methionine sulfoxide reductase B (MsrB) toFrancisella tularensisinfection in mice

2016 ◽  
Vol 364 (2) ◽  
pp. fnw260 ◽  
Author(s):  
Shib Shankar Saha ◽  
Masanori Hashino ◽  
Jin Suzuki ◽  
Akihiko Uda ◽  
Kenta Watanabe ◽  
...  
Keyword(s):  
Methionine Sulfoxide ◽  
Methionine Sulfoxide Reductase ◽  
Methionine Sulfoxide Reductase B

scholarly journals Plant Thioredoxin CDSP32 Regenerates 1-Cys Methionine Sulfoxide Reductase B Activity through the Direct Reduction of Sulfenic Acid

2010 ◽  
Vol 285 (20) ◽  
pp. 14964-14972 ◽  
Author(s):  
Lionel Tarrago ◽  
Edith Laugier ◽  
Mirko Zaffagnini ◽  
Christophe H. Marchand ◽  
Pierre Le Maréchal ◽  
...  
Keyword(s):  
Direct Reduction ◽  
Methionine Sulfoxide ◽  
Methionine Sulfoxide Reductase ◽  
Sulfenic Acid ◽  
Methionine Sulfoxide Reductase B

scholarly journals A Methionine Sulfoxide Reductase B Is Required for the Establishment of Astragalus sinicus–Mesorhizobium Symbiosis

2020 ◽  
Vol 61 (9) ◽  
pp. 1631-1645
Author(s):  
Zaiyong Si ◽  
Ning Guan ◽  
Yuan Zhou ◽  
Lingli Mei ◽  
Yixing Li ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Hairy Roots ◽  
Methionine Sulfoxide ◽  
Defense Responses ◽  
Methionine Sulfoxide Reductase ◽  
Bimolecular Fluorescence Complementation ◽  
Nodule Development ◽  
Astragalus Sinicus ◽  
Infection Threads ◽  
Methionine Sulfoxide Reductase B

Abstract Methionine sulfoxide reductase B (MsrB) is involved in oxidative stress or defense responses in plants. However, little is known about its role in legume–rhizobium symbiosis. In this study, an MsrB gene was identified from Astragalus sinicus and its function in symbiosis was characterized. AsMsrB was induced under phosphorus starvation and displayed different expression patterns under symbiotic and nonsymbiotic conditions. Hydrogen peroxide or methyl viologen treatment enhanced the transcript level of AsMsrB in roots and nodules. Subcellular localization showed that AsMsrB was localized in the cytoplasm of onion epidermal cells and co-localized with rhizobia in nodules. Plants with AsMsrB-RNAi hairy roots exhibited significant decreases in nodule number, nodule nitrogenase activity and fresh weight of the aerial part, as well as an abnormal nodule and symbiosome development. Statistical analysis of infection events showed that plants with AsMsrB-RNAi hairy roots had significant decreases in the number of root hair curling events, infection threads and nodule primordia compared with the control. The content of hydrogen peroxide increased in AsMsrB-RNAi roots but decreased in AsMsrB overexpression roots at the early stage of infection. The transcriptome analysis showed synergistic modulations of the expression of genes involved in reactive oxygen species generation and scavenging, defense and pathogenesis and early nodulation. In addition, a candidate protein interacting with AsMsrB was identified and confirmed by bimolecular fluorescence complementation. Taken together, our results indicate that AsMsrB plays an essential role in nodule development and symbiotic nitrogen fixation by affecting the redox homeostasis in roots and nodules.

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