Electric-Field-Induced Changes in Absorption and Fluorescence of the Green Fluorescent Protein Chromophore in a PMMA Film

2011 ◽  
Vol 115 (26) ◽  
pp. 8622-8626 ◽  
Author(s):  
Takakazu Nakabayashi ◽  
Kazuyuki Hino ◽  
Yuka Ohta ◽  
Sayuri Ito ◽  
Hirofumi Nakano ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Electric Field ◽  
Fluorescent Protein ◽  
Pmma Film ◽  
Green Fluorescent ◽  
Induced Changes

The Effect of an Electric Field on the Spectroscopic Properties of the Isolated Green Fluorescent Protein Chromophore Anion

ChemPhysChem ◽  
2018 ◽  
Vol 19 (14) ◽  
pp. 1686-1690 ◽  
Author(s):  
Jeppe Langeland ◽  
Christina Kjær ◽  
Lars H. Andersen ◽  
Steen Brøndsted Nielsen
Keyword(s):  
Green Fluorescent Protein ◽  
Electric Field ◽  
Fluorescent Protein ◽  
Spectroscopic Properties ◽  
Green Fluorescent

Electric field effect on the ground state proton transfer in the H-bonded HBDI complex: an implication of the green fluorescent protein

RSC Advances ◽  
2014 ◽  
Vol 4 (51) ◽  
pp. 26543-26551 ◽  
Author(s):  
Baotao Kang ◽  
Hongguang Liu ◽  
Du-Jeon Jang ◽  
Jin Yong Lee
Keyword(s):  
Green Fluorescent Protein ◽  
Electric Field ◽  
Proton Transfer ◽  
Ground State ◽  
First Principles ◽  
Field Effect ◽  
Fluorescent Protein ◽  
Electric Field Effect ◽  
First Principles Calculations ◽  
Green Fluorescent

In this paper, first-principles calculations were performed regarding the electric field effect on the ground state proton transfer (GSPT) in the H-bonded p-hydroxybenzylideneimidazolidinone (HBDI) network that represents the active site of the green fluorescent protein (GFP).

Electric field effects on fluorescence of the green fluorescent protein

2008 ◽  
Vol 457 (4-6) ◽  
pp. 408-412 ◽  
Author(s):  
Takakazu Nakabayashi ◽  
Masataka Kinjo ◽  
Nobuhiro Ohta
Keyword(s):  
Green Fluorescent Protein ◽  
Electric Field ◽  
Fluorescent Protein ◽  
Electric Field Effects ◽  
Field Effects ◽  
Green Fluorescent

scholarly journals The Effect of an Electric Field on the Spectroscopic Properties of the Isolated Green Fluorescent Protein Chromophore Anion

ChemPhysChem ◽  
2018 ◽  
Vol 19 (14) ◽  
pp. 1685-1685
Author(s):  
Jeppe Langeland ◽  
Christina Kjær ◽  
Lars H. Andersen ◽  
Steen Brøndsted Nielsen
Keyword(s):  
Green Fluorescent Protein ◽  
Electric Field ◽  
Fluorescent Protein ◽  
Spectroscopic Properties ◽  
Green Fluorescent

scholarly journals Cellular Leptin Resistance Impairs the Leptin-Mediated Suppression of Neuropeptide Y Secretion in Hypothalamic Neurons

Endocrinology ◽  
2011 ◽  
Vol 152 (11) ◽  
pp. 4138-4147 ◽  
Author(s):  
Sandeep S. Dhillon ◽  
Sean A. McFadden ◽  
Jennifer A. Chalmers ◽  
Maria-Luisa Centeno ◽  
Ginah L. Kim ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Neuropeptide Y ◽  
Energy Homeostasis ◽  
Fluorescent Protein ◽  
Primary Cultures ◽  
Leptin Resistance ◽  
Cellular Processes ◽  
Compound C ◽  
Green Fluorescent ◽  
Induced Changes

Evidence shows that neuropeptide Y (NPY) neurons are involved in mediating the anorexigenic action of leptin via neuronal circuits in the hypothalamus. However, studies have produced limited data on the cellular processes involved and whether hypothalamic NPY neurons are susceptible to cellular leptin resistance. To investigate the direct regulation of NPY secretion by leptin, we used novel NPY-synthesizing, immortalized mHypoA-NPY/green fluorescent protein and mHypoA-59 hypothalamic cell lines derived from adult hypothalamic primary cultures. We report that leptin treatment significantly suppressed NPY secretion in the cells by approximately 20%. We found a decrease in c-fos expression upon leptin exposure, indicating deactivation or hyperpolarization of the neurons. Protein analysis indicated that leptin inhibits AMP-activated protein kinase (AMPK) activity and activates acetyl-coenzyme A carboxylase in NPY neurons, supporting the hypothesis of an AMPK-dependent mechanism. Inhibiting both AMPK with Compound C or phosphatidylinositol 3 kinase (PI3K) with 2-(4-morpholinyl)-8-phenyl-1(4H)-1-benzopyran-4-one hydrochloride prevented the leptin-mediated decrease in NPY secretion, indicating both AMPK- and PI3K-mediated mechanisms. Further, NPY secretion was stimulated by 30% by the AMPK activator, aminoimidazole carboxamide ribonucleotide. Importantly, prolonged leptin exposure in the mHypoA-NPY/green fluorescent protein cells prevented leptin-induced changes in AMPK phosphorylation and suppression of NPY secretion, indicating that NPY neurons are susceptible to leptin resistance. Our studies indicate that AMPK and PI3K pathways are involved in leptin action in NPY neurons and that leptin resistance blocks the feedback response likely required to maintain energy homeostasis.

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