An Improved15N Relaxation Dispersion Experiment for the Measurement of Millisecond Time-Scale Dynamics in Proteins†

2008 ◽  
Vol 112 (19) ◽  
pp. 5898-5904 ◽  
Author(s):  
D. Flemming Hansen ◽  
Pramodh Vallurupalli ◽  
Lewis E. Kay
Keyword(s):  
Time Scale ◽  
Relaxation Dispersion ◽  
Millisecond Time Scale

13CHD2Methyl Group Probes of Millisecond Time Scale Exchange in Proteins by1H Relaxation Dispersion: An Application to Proteasome Gating Residue Dynamics

2010 ◽  
Vol 132 (32) ◽  
pp. 10992-10995 ◽  
Author(s):  
Andrew J. Baldwin ◽  
Tomasz L. Religa ◽  
D. Flemming Hansen ◽  
Guillaume Bouvignies ◽  
Lewis E. Kay
Keyword(s):  
Time Scale ◽  
Relaxation Dispersion ◽  
Millisecond Time Scale

Sensing oxygen at the millisecond time-scale using an ultra-microelectrode array (UMEA)

2017 ◽  
Vol 238 ◽  
pp. 1008-1016 ◽  
Author(s):  
Fleur van Rossem ◽  
Johan G. Bomer ◽  
Hans L. de Boer ◽  
Yawar Abbas ◽  
Eddy de Weerd ◽  
...  
Keyword(s):  
Time Scale ◽  
Microelectrode Array ◽  
Millisecond Time Scale

scholarly journals Routine Access to Millisecond Time Scale Events with Accelerated Molecular Dynamics

2012 ◽  
Vol 8 (9) ◽  
pp. 2997-3002 ◽  
Author(s):  
Levi C.T. Pierce ◽  
Romelia Salomon-Ferrer ◽  
Cesar Augusto F. de Oliveira ◽  
J. Andrew McCammon ◽  
Ross C. Walker
Keyword(s):  
Molecular Dynamics ◽  
Time Scale ◽  
Accelerated Molecular Dynamics ◽  
Millisecond Time Scale

scholarly journals Directly monitor protein rearrangement on a nanosecond-to-millisecond time-scale

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Eric H.-L. Chen ◽  
Tony T.-Y. Lu ◽  
Jack C.-C. Hsu ◽  
Yufeng Jane Tseng ◽  
T.-S. Lim ◽  
...  
Keyword(s):  
Time Scale ◽  
Millisecond Time Scale

scholarly journals Theta-Frequency Resonance at the Cerebellum Input Stage Improves Spike Timing on the Millisecond Time-Scale

2013 ◽  
Vol 7 ◽  
Author(s):  
Daniela Gandolfi ◽  
Paola Lombardo ◽  
Jonathan Mapelli ◽  
Sergio Solinas ◽  
Egidio D’Angelo
Keyword(s):  
Time Scale ◽  
Spike Timing ◽  
Input Stage ◽  
Frequency Resonance ◽  
Theta Frequency ◽  
Millisecond Time Scale

scholarly journals Modeling of excitatory amino acid transporters and clearance of synaptic cleft on millisecond time scale

2019 ◽  
Vol 14 (4) ◽  
pp. 407
Author(s):  
Denis Shchepakin ◽  
Leonid Kalachev ◽  
Michael Kavanaugh
Keyword(s):  
Amino Acid ◽  
Time Scale ◽  
Time Course ◽  
Transport Model ◽  
Excitatory Amino Acid ◽  
Synaptic Cleft ◽  
Amino Acid Transporters ◽  
Excitatory Amino Acid Transporters ◽  
Millisecond Time Scale ◽  
The Impact

Excitatory Amino Acid Transporters (EAATs) operate over wide time scales in the brain. They maintain low ambient concentrations of the primary excitatory amino acid neurotransmitter glutamate, but they also seem to play a significant role in clearing glutamate from the synaptic cleft in the millisecond time-scale process of chemical communication that occurs between neurons. The detailed kinetic mechanisms underlying glutamate uptake and clearance remain incompletely understood. In this work we used a combination of methods to model EAAT kinetics and gain insight into the impact of transport on glutamate dynamics in a general sense. We derive reliable estimates of the turnover rates of the three major EAAT subtypes expressed in the mammalian cerebral cortex. Previous studies have provided transporter kinetic estimates that vary over an order of magnitude. The values obtained in this study are consistent with estimates that suggest the unitary transporter rates are approximately 20-fold slower than the time course of glutamate in the synapse. A combined diffusion/transport model provides a possible mechanism for the apparent discrepancy.

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