In situ Electrochemical Fluorescence Studies of PPV

2006 ◽  
Vol 110 (51) ◽  
pp. 25791-25796 ◽  
Author(s):  
F. Montilla ◽  
R. Mallavia
Keyword(s):  
1991 ◽  
Vol 24 (19) ◽  
pp. 5451-5458 ◽  
Author(s):  
S. Mani ◽  
M. F. Malone ◽  
Henning H. Winter ◽  
J. L. Halary ◽  
L. Monnerie

2000 ◽  
Vol 48 (2) ◽  
pp. 219-227 ◽  
Author(s):  
Alison R. Hayman ◽  
Alison J. Bune ◽  
John R. Bradley ◽  
Jeremy Rashbass ◽  
Timothy M. Cox

Tartrate-resistant acid phosphatase (TRAP) is a histochemical marker of the osteoclast. It is also characteristic of monohistiocytes, particularly alveolar macrophages, and is associated with diverse pathological conditions, including hairy cell leukemia and AIDS encephalopathy. To study the biology of this enzyme, we investigated its expression and activity in mouse tissues. Confocal fluorescence studies showed that TRAP is localized to the lysosomal compartment of macrophages. In adult mice, high activities of the enzyme were demonstrated in bone, spleen, liver, thymus, and colon, with lower amounts in lung, stomach, skin, brain, and kidney. Trace amounts were detected in testis, muscle, and heart. Expression of TRAP mRNA was investigated in tissue sections by in situ hybridization and protein expression was monitored by histochemical staining or immunohistochemically. TRAP is widely expressed in many tissues, where it is associated with cells principally originating from the bone marrow, including those of osteoclast/macrophage lineage. The cellular distribution of TRAP mRNA and enzyme antigen in the tissues corresponds closely to that of cells staining with an antibody directed to the CD80 (B7) antigen. Therefore, to confirm its putative localization in dendritic cells, isolated bone marrow dendritic cells were matured in culture. These co-stained strongly for TRAP protein and the CD80 antigen. These studies demonstrate that TRAP is a lysosomal enzyme that is found in diverse murine tissues, where it is expressed in dendritic cells as well as osteoclasts and macrophages, as previously shown.


Biochemistry ◽  
2003 ◽  
Vol 42 (15) ◽  
pp. 4333-4348 ◽  
Author(s):  
Pascal Mercier ◽  
Roisean E. Ferguson ◽  
Malcolm Irving ◽  
John E. T. Corrie ◽  
David R. Trentham ◽  
...  

2009 ◽  
Vol 78 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Jelena Zvezdanović ◽  
Tijana Cvetić ◽  
Sonja Veljović-Jovanović ◽  
Dejan Marković

1992 ◽  
Vol 53 (1) ◽  
pp. 95-107 ◽  
Author(s):  
S. Balaei ◽  
J.J. Aaron ◽  
A. Desbene-Monvernay ◽  
P.C. Lacaze
Keyword(s):  
Ex Situ ◽  

2010 ◽  
Vol 5 (3) ◽  
pp. 290-294
Author(s):  
Eko Sri Kunarti

Monitoring of hydrolysis and condensation polymerization reactions of tetramethylorthosilicate-organosiloxane have been carried out. Proton transfer from the excited state of 8-hydroxy-1,3,6-pyrenetrisulfonic acid trisodium salt (pyranine) to surrounding water molecules was used as a sensitive fluorescence method for following the kinetics of water consumption during the early stages of the sol-gel polymerisation process. Changes in water/silane ratio, type of organosiloxane, mol ratio of organosiloxane to silica precursor and pH affected markedly the kinetic behavior of the hydrolysis and condensation reaction of tetramethylorthosilicate.   Keywords: hydrolysis, polymerisation, fluorescence, pyranine, tetramethylorthosilicate, organosiloxane.


1969 ◽  
Vol 22 (9) ◽  
pp. 1899 ◽  
Author(s):  
IH Leaver ◽  
GC Ramsay

Indoles, phenols, and thiols have been shown to undergo photosensitization by a diphenylpyrazoline in non-polar solvents. The intermediate radicals produced have been trapped using a nitrosoalkane scavenger, and the resulting nitroxide radicals examined in situ by e.s.r. Fluorescence studies of these systems have shown that indoles and phenols strongly quench the singlet excited state of the diphenyl- pyrazoline, whereas thiols have no effect.


1984 ◽  
Vol 75 ◽  
pp. 743-759 ◽  
Author(s):  
Kerry T. Nock

ABSTRACTA mission to rendezvous with the rings of Saturn is studied with regard to science rationale and instrumentation and engineering feasibility and design. Future detailedin situexploration of the rings of Saturn will require spacecraft systems with enormous propulsive capability. NASA is currently studying the critical technologies for just such a system, called Nuclear Electric Propulsion (NEP). Electric propulsion is the only technology which can effectively provide the required total impulse for this demanding mission. Furthermore, the power source must be nuclear because the solar energy reaching Saturn is only 1% of that at the Earth. An important aspect of this mission is the ability of the low thrust propulsion system to continuously boost the spacecraft above the ring plane as it spirals in toward Saturn, thus enabling scientific measurements of ring particles from only a few kilometers.


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