Fluorescence Study of DNA Binding and Bending byEcoRI DNA Methyltransferase

2006 ◽  
Vol 110 (39) ◽  
pp. 19647-19651
Author(s):  
Baocheng Ma ◽  
Jun Wang ◽  
Xiaohong Fang
Keyword(s):  
Dna Binding ◽  
Dna Methyltransferase ◽  
Fluorescence Study ◽  
Dna Binding And Bending

scholarly journals Lowering DNA binding affinity of SssI DNA methyltransferase does not enhance the specificity of targeted DNA methylation in E. coli

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Krystyna Ślaska-Kiss ◽  
Nikolett Zsibrita ◽  
Mihály Koncz ◽  
Pál Albert ◽  
Ákos Csábrádi ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Dna Binding ◽  
Binding Affinity ◽  
Dna Methyltransferase ◽  
Restriction Enzymes ◽  
Dna Binding Protein ◽  
E Coli ◽  
Dna Binding Affinity ◽  
Higher Eukaryotes

AbstractTargeted DNA methylation is a technique that aims to methylate cytosines in selected genomic loci. In the most widely used approach a CG-specific DNA methyltransferase (MTase) is fused to a sequence specific DNA binding protein, which binds in the vicinity of the targeted CG site(s). Although the technique has high potential for studying the role of DNA methylation in higher eukaryotes, its usefulness is hampered by insufficient methylation specificity. One of the approaches proposed to suppress methylation at unwanted sites is to use MTase variants with reduced DNA binding affinity. In this work we investigated how methylation specificity of chimeric MTases containing variants of the CG-specific prokaryotic MTase M.SssI fused to zinc finger or dCas9 targeting domains is influenced by mutations affecting catalytic activity and/or DNA binding affinity of the MTase domain. Specificity of targeted DNA methylation was assayed in E. coli harboring a plasmid with the target site. Digestions of the isolated plasmids with methylation sensitive restriction enzymes revealed that specificity of targeted DNA methylation was dependent on the activity but not on the DNA binding affinity of the MTase. These results have implications for the design of strategies of targeted DNA methylation.

scholarly journals Cooperative DNA Binding and Protein/DNA Fiber Formation Increases the Activity of the Dnmt3a DNA Methyltransferase

2014 ◽  
Vol 289 (43) ◽  
pp. 29602-29613 ◽  
Author(s):  
Max Emperle ◽  
Arumugam Rajavelu ◽  
Richard Reinhardt ◽  
Renata Z. Jurkowska ◽  
Albert Jeltsch
Keyword(s):  
Dna Binding ◽  
Dna Methyltransferase ◽  
Fiber Formation

scholarly journals DNA Binding and Bending by the Human Papillomavirus Type 16 E2 Protein

1997 ◽  
Vol 272 (13) ◽  
pp. 8236-8242 ◽  
Author(s):  
Alison Thain ◽  
Kenneth Webster ◽  
Dave Emery ◽  
Anthony R. Clarke ◽  
Kevin Gaston
Keyword(s):  
Human Papillomavirus ◽  
Dna Binding ◽  
E2 Protein ◽  
Human Papillomavirus Type 16 ◽  
Dna Binding And Bending

H3K36me2/3 Binding and DNA Binding of the DNA Methyltransferase DNMT3A PWWP Domain Both Contribute to its Chromatin Interaction

2019 ◽  
Vol 431 (24) ◽  
pp. 5063-5074 ◽  
Author(s):  
Michael Dukatz ◽  
Katharina Holzer ◽  
Michel Choudalakis ◽  
Max Emperle ◽  
Cristiana Lungu ◽  
...  
Keyword(s):  
Dna Binding ◽  
Dna Methyltransferase ◽  
Chromatin Interaction ◽  
Pwwp Domain

scholarly journals Human placental DNA methyltransferase: DNA substrate and DNA binding specificity

1984 ◽  
Vol 12 (8) ◽  
pp. 3473-3490 ◽  
Author(s):  
Richard Y.-H. Wang ◽  
Lan-Hsiang Huang ◽  
Melanie Ehrlich
Keyword(s):  
Dna Binding ◽  
Dna Methyltransferase ◽  
Binding Specificity ◽  
Dna Binding Specificity ◽  
Dna Substrate

Single-stranded DNA binding and methylation by EcoP1I DNA methyltransferase

2004 ◽  
Vol 314 (1) ◽  
pp. 159-165 ◽  
Author(s):  
Srivani Sistla ◽  
Vinita Krishnamurthy ◽  
Desirazu N Rao
Keyword(s):  
Dna Binding ◽  
Dna Methyltransferase ◽  
Single Stranded Dna

Ananlysis of the DNA binding and bending activity of M.EcoRV DNA-(adenine N6)-methyltransferase

2007 ◽  
Vol 2007 (Fall) ◽  
Author(s):  
Nils Anspach ◽  
Tomasz P. Jurkowski ◽  
Liliya Kulishova ◽  
Albert Jeltsch ◽  
Wolfgang Nellen
Keyword(s):  
Dna Binding ◽  
Dna Binding And Bending

Global analysis of ion dependence unveils hidden steps in DNA binding and bending by integration host factor

2013 ◽  
Vol 139 (12) ◽  
pp. 121927 ◽  
Author(s):  
Paula Vivas ◽  
Yogambigai Velmurugu ◽  
Serguei V. Kuznetsov ◽  
Phoebe A. Rice ◽  
Anjum Ansari
Keyword(s):  
Dna Binding ◽  
Global Analysis ◽  
Integration Host Factor ◽  
Host Factor ◽  
Dna Binding And Bending

Study of DNA binding and bending by Bacillus subtilis GabR, a PLP-dependent transcription factor

2017 ◽  
Vol 1861 (1) ◽  
pp. 3474-3489 ◽  
Author(s):  
Davide Amidani ◽  
Angela Tramonti ◽  
Andrea Valeria Canosa ◽  
Barbara Campanini ◽  
Stefano Maggi ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Bacillus Subtilis ◽  
Dna Binding ◽  
Dna Binding And Bending

scholarly journals The DNA Binding and Bending Activities of Truncated Tail-less HMGB1 protein are Differentially Affected by Lys-2 and Lys-81 Residues and Their Acetylation

2011 ◽  
Vol 7 (6) ◽  
pp. 691-699 ◽  
Author(s):  
Ivan Elenkov ◽  
Petar Pelovsky ◽  
Iva Ugrinova ◽  
Masayuki Takahashi ◽  
Evdokia Pasheva
Keyword(s):  
Dna Binding ◽  
Hmgb1 Protein ◽  
Dna Binding And Bending
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