scholarly journals Fluorescence Correlation Spectroscopy Simulations of Photophysical Phenomena and Molecular Interactions:  A Molecular Dynamics/Monte Carlo Approach

2006 ◽  
Vol 110 (4) ◽  
pp. 1896-1906 ◽  
Author(s):  
James A. Dix ◽  
Erik F. Y. Hom ◽  
A. S. Verkman
Keyword(s):  
Molecular Dynamics ◽  
Monte Carlo ◽  
Molecular Interactions ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Monte Carlo Approach ◽  
Fluorescence Correlation

Monte Carlo simulation of fluorescence correlation spectroscopy data

2011 ◽  
Vol 76 (3) ◽  
pp. 207-222 ◽  
Author(s):  
Peter Košovan ◽  
Filip Uhlík ◽  
Jitka Kuldová ◽  
Miroslav Štěpánek ◽  
Zuzana Limpouchová ◽  
...  
Keyword(s):  
Monte Carlo Simulation ◽  
Monte Carlo ◽  
Diffusion Coefficients ◽  
Fluorescence Correlation Spectroscopy ◽  
Rotational Diffusion ◽  
Correlation Spectroscopy ◽  
Analytical Models ◽  
Spherical Particles ◽  
Fluorescence Signal ◽  
Fluorescence Correlation

We employed the Monte Carlo simulation methodology to emulate the diffusion of fluorescently labeled particles and understand the source of differences between values of diffusion coefficients (and consequently hydrodynamic radii) of fluorescently labeled nanoparticles measured by fluorescence correlation spectroscopy (FCS) and dynamic light scattering (DLS). We used the simulation program developed in our laboratory and studied the diffusion of spherical particles of different sizes, which are labeled on their surface. In this study, we focused on two complicating effects: (i) multiple labeling and (ii) rotational diffusion which affect the fluorescence signal from large particles and hinder the analysis of autocorrelation functions according to simple analytical models. We have shown that the fluorescence fluctuations can be well fitted using the analytical model for small point-like particles, but the obtained parameters deviate in some cases significantly from the real ones. It means that the current data treatment yields apparent values of diffusion coefficients and other parameters only and the interpretation of experimental results for systems of particles with sizes comparable to the size of the active illuminated volume requires great care and precaution.

Numerical Fluorescence Correlation Spectroscopy for the Analysis of Molecular Dynamics under Nonstandard Conditions

2007 ◽  
Vol 79 (11) ◽  
pp. 4031-4039 ◽  
Author(s):  
Michael J. Culbertson ◽  
Joshua T. B. Williams ◽  
Wayland W. L. Cheng ◽  
Dee Ann Stults ◽  
Emily R. Wiebracht ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Nonstandard Conditions

scholarly journals Nanowaveguide-Illuminated Fluorescence Correlation Spectroscopy for Studying Molecular Dynamics on Cell Membranes

2020 ◽  
Vol 118 (3) ◽  
pp. 141a
Author(s):  
Joseph M. Chandler ◽  
Patrick DeLear ◽  
Brian Le ◽  
Chelsea Howard ◽  
Arstanbek Tulekeyev ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Fluorescence Correlation Spectroscopy ◽  
Cell Membranes ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation
Sign in / Sign up

Export Citation Format

Share Document