Single Molecule Nanoparticles of the Conjugated Polymer MEH−PPV, Preparation and Characterization by Near-Field Scanning Optical Microscopy

2005 ◽  
Vol 109 (18) ◽  
pp. 8543-8546 ◽  
Author(s):  
Craig Szymanski ◽  
Changfeng Wu ◽  
Joseph Hooper ◽  
Mary Alice Salazar ◽  
Alejandro Perdomo ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Single Molecule ◽  
Conjugated Polymer ◽  
Near Field ◽  
Scanning Optical Microscopy ◽  
Near Field Scanning

scholarly journals Cell biology beyond the diffraction limit: near-field scanning optical microscopy

2001 ◽  
Vol 114 (23) ◽  
pp. 4153-4160
Author(s):  
Frank de Lange ◽  
Alessandra Cambi ◽  
Richard Huijbens ◽  
Bärbel de Bakker ◽  
Wouter Rensen ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Single Molecule ◽  
Cell Biology ◽  
Imaging Techniques ◽  
Near Field ◽  
Diffraction Limit ◽  
Detection Sensitivity ◽  
Live Cells ◽  
Scanning Optical Microscopy ◽  
Near Field Scanning

Throughout the years, fluorescence microscopy has proven to be an extremely versatile tool for cell biologists to study live cells. Its high sensitivity and non-invasiveness, together with the ever-growing spectrum of sophisticated fluorescent indicators, ensure that it will continue to have a prominent role in the future. A drawback of light microscopy is the fundamental limit of the attainable spatial resolution – ∼250 nm – dictated by the laws of diffraction. The challenge to break this diffraction limit has led to the development of several novel imaging techniques. One of them, near-field scanning optical microscopy (NSOM), allows fluorescence imaging at a resolution of only a few tens of nanometers and, because of the extremely small near-field excitation volume, reduces background fluorescence from the cytoplasm to the extent that single-molecule detection sensitivity becomes within reach. NSOM allows detection of individual fluorescent proteins as part of multimolecular complexes on the surface of fixed cells, and similar results should be achievable under physiological conditions in the near future.

scholarly journals Single Molecule Rotational and Translational Diffusion Observed by Near-Field Scanning Optical Microscopy

1997 ◽  
Vol 101 (40) ◽  
pp. 7318-7323 ◽  
Author(s):  
A. G. T. Ruiter ◽  
J. A. Veerman ◽  
M. F. Garcia-Parajo ◽  
N. F. van Hulst
Keyword(s):  
Optical Microscopy ◽  
Single Molecule ◽  
Near Field ◽  
Translational Diffusion ◽  
Scanning Optical Microscopy ◽  
Near Field Scanning
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