Highly Efficient Spectral Hole-Burning in Oxygen-Evolving Photosystem II Preparations†

2004 ◽  
Vol 108 (29) ◽  
pp. 10428-10439 ◽  
Author(s):  
Joseph L. Hughes ◽  
Barry J. Prince ◽  
Elmars Krausz ◽  
Paul J. Smith ◽  
Ron J. Pace ◽  
...  
2004 ◽  
Vol 108 (1-4) ◽  
pp. 101-105 ◽  
Author(s):  
Barry J. Prince ◽  
Elmars Krausz ◽  
Peterson Årsköld Sindra ◽  
Paul J. Smith ◽  
Ron J. Pace

2002 ◽  
Vol 98 (1-4) ◽  
pp. 131-139
Author(s):  
Y Kawamata ◽  
S Machida ◽  
K Horie ◽  
S Itoh ◽  
M Iwaki ◽  
...  

2006 ◽  
Vol 119-120 ◽  
pp. 298-303 ◽  
Author(s):  
Joseph L. Hughes ◽  
Paul J. Smith ◽  
Ron J. Pace ◽  
Elmars Krausz

2004 ◽  
Vol 57 (12) ◽  
pp. 1179 ◽  
Author(s):  
Joseph L. Hughes ◽  
Barry J. Prince ◽  
Sindra Peterson Årsköld ◽  
Paul J. Smith ◽  
Ron J. Pace ◽  
...  

Low-temperature spectra of fully active (oxygen-evolving) Photosystem II (PSII) cores prepared from spinach exhibit well developed structure. Spectra of isolated sub-fragments of PSII cores establish that the native reaction centre is better structured and red-shifted compared to the isolated reaction centre. Laser illumination of PSII cores leads to efficient and deep spectral hole-burning. Measurements of homogeneous hole-widths establish excited-state lifetimes in the 40–300 ps range. The high hole-burning efficiency is attributed to charge separation of P680 in native PSII that follows reaction-centre excitation via ‘slow transfer’ states in the inner light-harvesting assemblies CP43 and CP47. The ‘slow transfer’ state in CP47 and that in CP43 can be distinguished in the hole-burning action spectrum and high-resolution hole-burning spectra. An important observation is that 685–700 nm illumination gives rise to efficient P680 charge separation, as established by QA− formation. This leads to a new paradigm for P680. The charge-separating state has surprisingly weak absorption and extends to 700 nm.


1987 ◽  
Vol 48 (C5) ◽  
pp. C5-511-C5-515 ◽  
Author(s):  
J. L. OUDAR ◽  
J. DUBARD ◽  
F. ALEXANDRE ◽  
D. HULIN ◽  
A. MIGUS ◽  
...  

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