Small-Angle X-ray Scattering, Light Scattering, and NMR Study of PEO−PPO−PEO Triblock Copolymer/Cationic Surfactant Complexes in Aqueous Solution

2005 ◽  
Vol 109 (15) ◽  
pp. 7073-7083 ◽  
Author(s):  
Jörgen Jansson ◽  
Karin Schillén ◽  
Markus Nilsson ◽  
Olle Söderman ◽  
Gerhard Fritz ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Light Scattering ◽  
Cationic Surfactant ◽  
Small Angle ◽  
Triblock Copolymer ◽  
X Ray ◽  
X Ray Scattering ◽  
Nmr Study ◽  
Scattering Light ◽  
Ray Scattering

A Small-Angle X-Ray Scattering Study on Pre-Irradiated Malate Synthase. The Influence of Formate, Superoxide Dismutase, and Catalase on the X-Ray Induced Aggregation of the Enzyme

1985 ◽  
Vol 40 (5-6) ◽  
pp. 364-372 ◽  
Author(s):  
P. Zipper ◽  
R. Wilfing ◽  
M. Kriechbaum ◽  
H. Durchschlag
Keyword(s):  
Aqueous Solution ◽  
Superoxide Dismutase ◽  
Small Angle ◽  
Malate Synthase ◽  
X Ray ◽  
X Ray Scattering ◽  
X Irradiation ◽  
The Mean ◽  
Induced Aggregation ◽  
Ray Scattering

Abstract The sulfhydryl enzyme malate synthase from baker’s yeast was X-irradiated with 6 kGy in air-saturated aqueous solution (enzyme concentration: ≃ 10 mg/ml; volume: 120 μl), in the absence or presence of the specific scavengers formate, superoxide dismutase, and catalase. After X-irradiation, a small aliquot of the irradiated solutions was tested for enzymic activity while the main portion was investigated by means of small-angle X-ray scattering. Additionally, an unir­radiated sample without additives was investigated as a reference. Experiments yielded the fol­lowing results: 1. X-irradiation in the absence of the mentioned scavengers caused considerable aggregation, fragmentation, and inactivation of the enzyme. The dose Dt37 for total (= repairable + non­-repayable) inactivation resulted as 4.4 kGy. The mean radius of gyration was found to be about 13 nm. The mean degree of aggregation was obtained as 5.7, without correction for fragmenta­tion. An estimation based on the thickness factor revealed that about 19% of material might be strongly fragmented. When this amount of fragments was accordingly taken into account, a value of 7.1 was obtained as an upper limit for the mean degree of aggregation. The observed retention of the thickness factor and the finding of two different cross-section factors are in full accord with the two-dimensional aggregation model established previously (Zipper and Durchschlag, Radiat. Environ. Biophys. 18, 99 - 121 (1980)). 2. The presence of catalytic amounts of superoxide dismutase and/or catalase, in the absence of formate, during X-irradiation reduced both aggregation and inactivation significantly. 3. The presence of formate (10 or 100 mᴍ) during X-irradiation led to a strong decrease of aggregation and inactivation. This effect was more pronounced with the higher formate concen­tration or when superoxide dismutase and/or catalase were simultaneously present during X-irradiation. The presence of formate also reduced the amount of fragments significantly. 4. The results clearly show that the aggregation and inactivation of malate synthase upon X-irradiation in aqueous solution are mainly caused by OH·; to a minor extent O·̄2 and H2O2 are additionally involved in the damaging processes.

Chain Conformation of Poly(d-lactide) in Tetrahydrofuran by Static Light Scattering, Small-Angle X-ray Scattering, and Intrinsic Viscosity

2020 ◽  
Vol 53 (5) ◽  
pp. 1604-1612 ◽  
Author(s):  
Yoshinori Suzuki ◽  
Takahiro Watanabe ◽  
Hiroki Kosugi ◽  
Kayo Ueda ◽  
Moriya Kikuchi ◽  
...  
Keyword(s):  
Light Scattering ◽  
Intrinsic Viscosity ◽  
Small Angle ◽  
Chain Conformation ◽  
Static Light Scattering ◽  
X Ray ◽  
X Ray Scattering ◽  
Ray Scattering

Small-Angle X-ray Scattering and Light Scattering on Lysozyme and Sodium Glycocholate Micelles

2005 ◽  
Vol 109 (50) ◽  
pp. 23857-23869 ◽  
Author(s):  
Claudia Leggio ◽  
Luciano Galantini ◽  
Emanuela Zaccarelli ◽  
Nicolae Viorel Pavel
Keyword(s):  
Light Scattering ◽  
Small Angle ◽  
Sodium Glycocholate ◽  
X Ray ◽  
X Ray Scattering ◽  
Ray Scattering

scholarly journals Use of dynamic light scattering and small-angle X-ray scattering to characterize new surfactants in solution conditions for membrane-protein crystallization

2015 ◽  
Vol 71 (7) ◽  
pp. 838-846 ◽  
Author(s):  
Mohamed Dahani ◽  
Laurie-Anne Barret ◽  
Simon Raynal ◽  
Colette Jungas ◽  
Pétra Pernot ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Membrane Protein ◽  
Small Angle ◽  
Protein Crystallization ◽  
X Ray ◽  
X Ray Scattering ◽  
Membrane Protein Crystallization ◽  
Surfactant Phase ◽  
Ray Scattering

The structural and interactive properties of two novel hemifluorinated surfactants, F2H9-β-M and F4H5-β-M, the syntheses of which were based on the structure and hydrophobicity of the well known dodecyl-β-maltoside (DD-β-M), are described. The shape of their micellar assemblies was characterized by small-angle X-ray scattering and their intermicellar interactions in crystallizing conditions were measured by dynamic light scattering. Such information is essential for surfactant phase-diagram determination and membrane-protein crystallization.

Coaggregation of Two Anionic Azo Dyestuffs: A Combined Static Light Scattering and Small-Angle X-ray Scattering Study

2014 ◽  
Vol 118 (27) ◽  
pp. 7618-7629 ◽  
Author(s):  
Rolf Michels ◽  
Günter Goerigk ◽  
Ulla Vainio ◽  
Jérémie Gummel ◽  
Klaus Huber
Keyword(s):  
Light Scattering ◽  
Small Angle ◽  
Static Light Scattering ◽  
X Ray ◽  
X Ray Scattering ◽  
Scattering Study ◽  
Ray Scattering

Human Serum Albumin Unfolding: A Small-Angle X-ray Scattering and Light Scattering Study

2008 ◽  
Vol 112 (48) ◽  
pp. 15460-15469 ◽  
Author(s):  
Luciano Galantini ◽  
Claudia Leggio ◽  
Nicolae Viorel Pavel
Keyword(s):  
Light Scattering ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Small Angle ◽  
X Ray ◽  
X Ray Scattering ◽  
Scattering Study ◽  
Ray Scattering
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