Dynamics of β-Carotene-to-Chlorophyll Singlet Energy Transfer in the Core of Photosystem II

2003 ◽  
Vol 107 (25) ◽  
pp. 6214-6220 ◽  
Author(s):  
Frank L. de Weerd ◽  
Jan P. Dekker ◽  
Rienk van Grondelle
Keyword(s):  
Energy Transfer ◽  
Photosystem Ii ◽  
The Core ◽  
Β Carotene

Immunological Evidence for the Binding of β-Carotene and Xanthophylls onto Peptides of Photosystem I from Nicotiana tabacum

1994 ◽  
Vol 49 (7-8) ◽  
pp. 427-438 ◽  
Author(s):  
A. Makewicz ◽  
A. Radunz ◽  
G. H. Schmid
Keyword(s):  
Nicotiana Tabacum ◽  
Photosystem Ii ◽  
Western Blot ◽  
Photosystem I ◽  
Light Harvesting ◽  
Wild Type ◽  
The Core ◽  
Ps I ◽  
Molecular Masses ◽  
Β Carotene

Photosystem I preparations were obtained from wild type tobacco Nicotiana tabacum var. John William’s Broadleaf (JWB) and from the two chlorophyll-deficient mutants N. tabacum Su/su and N. tabacum Su/su var. Aurea. The preparations were characterized with respect to the chlorophyll a/b ratio, their photosynthetic activity and their absorption spectroscopic properties. Peptides from these preparations were analyzed by SDS polyacrylamide gel electrophoresis and transferred for the detection of bound carotenoids according to the Western blot procedure to nitrocellulose or Immobilon membranes. The PS I preparation from the wild type JWB consisted of the core and the LHCP complex. The core complex contains the two core peptides with the same apparent MW of 66 kDa and several peptides with the lesser molecular masses of 22, 20, 19, 17, 16, 10 and 9 kDa. The light-harvesting protein complex consists of 4 subunits with the molecular masses 28, 26, 25 and 24 kDa. The PS I preparations of the yellow-green mutant Su/su and of the Aurea mutant Su/su var. Aurea contain as impurity traces of the D1 and D2 core peptides of photosystem II and also traces of the chlorophyll-binding photosystem II peptides with the molecular masses 42 and 47 kDa. The peptides of the photosystem I preparation were characterized by specific photosystem I antisera: An antiserum to the photosystem I complex reacts in the Western blot only with the homologous peptides of photosystem I. In comparative analyses with photosystem II preparations this antiserum (directed to photosystem I) reacts, as expected, only with the peptides of the light-harvesting complex. An antiserum to the CP 1 core peptides reacts only with the 66 kDa peptides of photosystem I and gives no cross reaction with heterodimer forms of the D1/D2 core peptides of photosystem II. In the Western blot procedure by means of polyclonal monospecific antisera to carotenoids it was demonstrated that β-carotene is bound in high concentration onto the core peptides CP 1 and to a lesser extent onto the two larger subunits of the LHCP complex, exhibiting the molecular masses of 28 and 26 kDa. Neoxanthin is bound onto the same peptides. In contrast to this, lutein was only identified on the core peptides CP 1 and violaxanthin only on the larger subunits of the LHCP complex. As the carotenoids are labelled with antibodies, even after SDS treatment in the electrophoresis, it is assumed, that the carotenoids are covalently bound via the ionon ring to the respective peptide

β-Carotene Probes the Energy Transfer Pathway in the Photosystem II Core Complex

2019 ◽  
Vol 10 (13) ◽  
pp. 3710-3714 ◽  
Author(s):  
Yusuke Yoneda ◽  
Yutaka Nagasawa ◽  
Yasufumi Umena ◽  
Hiroshi Miyasaka
Keyword(s):  
Energy Transfer ◽  
Photosystem Ii ◽  
Core Complex ◽  
Energy Transfer Pathway ◽  
Β Carotene

scholarly journals Pathways for Energy Transfer in the Core Light-Harvesting Complexes CP43 and CP47 of Photosystem II

2002 ◽  
Vol 82 (3) ◽  
pp. 1586-1597 ◽  
Author(s):  
Frank L. de Weerd ◽  
Ivo H.M. van Stokkum ◽  
Herbert van Amerongen ◽  
Jan P. Dekker ◽  
Rienk van Grondelle
Keyword(s):  
Energy Transfer ◽  
Photosystem Ii ◽  
Light Harvesting ◽  
Light Harvesting Complexes ◽  
The Core

Quantitative Changes of the Lipid and Fatty Acid Composition of Leaves of Aleurites montana as a Consequence of Growth under 700 ppm CO2 in the Atmosphere

1996 ◽  
Vol 51 (11-12) ◽  
pp. 833-840 ◽  
Author(s):  
P He ◽  
A Radunz ◽  
K. P Bader ◽  
G. H Schmid
Keyword(s):  
Fatty Acids ◽  
Thylakoid Membrane ◽  
Saturated Fatty Acids ◽  
Phospholipid Fraction ◽  
Phospholipid Content ◽  
The Core ◽  
Glycolipid Fraction ◽  
Carbonic Acids ◽  
And Control ◽  
Β Carotene

Abstract Leaf lipids of Aleurites plants that were cultivated for 5 months in air containing 700 ppm CO2, were compared to those of control plants cultivated at 350 ppm CO2. The content of ether soluble lipids referred to dry matter is the same in CO2-and control plants. The comparison of lipids analyzed as the pigments chlorophyll and carotenoids, phospholipids and glycolipids shows that the ratio of phospholipids and glycolipids is slightly shifted in favor of phospholipids in CO2-plants. Thus, within the group of phospholipids, phosphatidylglycerol and phosphatidylinositol occur in higher concentrations in CO2-plants. Although the differences in the lipid content appear moderate in CO2-and control plants, it is the saturation degree of fatty acids that differs substantially. The fatty acids of CO2-plants contain according to the higher phospholipid content approx. 5% more saturated fatty acids. Stearic acid is three-fold increased. Whereas in the phospholipid fraction saturated fatty acids comprise one half of all fatty acids, the unsaturated fatty acids make up for 80 to 90% in the glycolipid fraction. In CO2-plants not only in the phospholipid fraction but also in the glycolipid fraction saturated fatty acids occur in a higher portion. This means that not only in the cell membrane of CO2-plants but also in the thylakoid membrane the fluidity is decreased. Also in the wax-fraction long-chained carbonic acids with 20 -26 carbon atoms occur. As the portion of these carbonic acids is twice as high in CO2-plants, it is concluded that a stronger formation of the wax layers exists in CO2-plants. By means of Western blotting and by the use of lipid and carotenoid antisera the binding of lipids onto proteins of photosystem II and photosystem I was analyzed. It is seen that besides the major amount of lipids which build up the thylakoid membrane, some lipids are also bound to membrane peptides. Whereas monogalactolipid is bound to the LHCP-complex peptides, to the OEC1 -peptide and the 43 and 47 kDa chlorophyll binding peptides, the anionic lipids sulfoquinovosyldiglyceride and phosphatidylglycerol and digalactolipid are bound to the core peptides of PS II and PS I. β-carotene and the xanthophylls were found to be bound to the core peptides and β-carotene and violaxanthin were also bound to the light-harvesting pigment complex.

scholarly journals Energy Transfer Pathways in the Minor Antenna Complex CP29 of Photosystem II: A Femtosecond Study of Carotenoid to Chlorophyll Transfer on Mutant and WT Complexes

2003 ◽  
Vol 84 (4) ◽  
pp. 2517-2532 ◽  
Author(s):  
Roberta Croce ◽  
Marc G. Müller ◽  
Stefano Caffarri ◽  
Roberto Bassi ◽  
Alfred R. Holzwarth
Keyword(s):  
Energy Transfer ◽  
Photosystem Ii ◽  
Antenna Complex
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