Controlled Site-Selective Glycosylation of Proteins by a Combined Site-Directed Mutagenesis and Chemical Modification Approach

Benjamin G. Davis; Richard C. Lloyd; J. Bryan Jones

doi:10.1021/jo9816461

Controlled Site-Selective Glycosylation of Proteins by a Combined Site-Directed Mutagenesis and Chemical Modification Approach

The Journal of Organic Chemistry ◽

10.1021/jo9816461 ◽

1998 ◽

Vol 63 (26) ◽

pp. 9614-9615 ◽

Cited By ~ 57

Author(s):

Benjamin G. Davis ◽

Richard C. Lloyd ◽

J. Bryan Jones

Keyword(s):

Chemical Modification ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Site Selective

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The role of cysteine 206 in allosteric inhibition of Escherichia coli citrate synthase. Studies by chemical modification, site-directed mutagenesis, and 19F NMR.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)54766-9 ◽

1991 ◽

Vol 266 (31) ◽

pp. 20709-20713

Author(s):

L.J. Donald ◽

B.R. Crane ◽

D.H. Anderson ◽

H.W. Duckworth

Keyword(s):

Escherichia Coli ◽

Chemical Modification ◽

Citrate Synthase ◽

Site Directed Mutagenesis ◽

19F Nmr ◽

Directed Mutagenesis ◽

Allosteric Inhibition

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Structure-function relationships of cyanobacterial ADP-glucose pyrophosphorylase. Site-directed mutagenesis and chemical modification of the activator-binding sites of ADP-glucose pyrophosphorylase from Anabaena PCC 7120.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)51054-7 ◽

1994 ◽

Vol 269 (39) ◽

pp. 24107-24113

Author(s):

Y.Y. Charng ◽

A.A. Iglesias ◽

J. Preiss

Keyword(s):

Chemical Modification ◽

Structure Function ◽

Binding Sites ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Anabaena Pcc 7120 ◽

Adp Glucose Pyrophosphorylase ◽

Pcc 7120

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Studies on the reaction mechanism of a microbial lipase/acyltransferase using chemical modification and site-directed mutagenesis.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)35273-0 ◽

1991 ◽

Vol 266 (2) ◽

pp. 997-1000 ◽

Cited By ~ 1

Author(s):

S Hilton ◽

J T Buckley

Keyword(s):

Reaction Mechanism ◽

Chemical Modification ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Microbial Lipase

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Structure-Function Studies of Escherichia coli Biotin Synthase via a Chemical Modification and Site-Directed Mutagenesis Approach

The Journal of Biochemistry ◽

10.1093/oxfordjournals.jbchem.a003028 ◽

2001 ◽

Vol 130 (5) ◽

pp. 627-635 ◽

Cited By ~ 7

Author(s):

L. Farh ◽

S.-Y. Hwang ◽

L. Steinrauf ◽

H.-J. Chiang ◽

D. Shiuan

Keyword(s):

Escherichia Coli ◽

Chemical Modification ◽

Structure Function ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Biotin Synthase

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Identification of the cysteine in the steroid-binding site of human corticosteroid binding globulin by site-directed mutagenesis and site-specific chemical modification

The Journal of Steroid Biochemistry and Molecular Biology ◽

10.1016/0960-0760(94)90260-7 ◽

1994 ◽

Vol 48 (1) ◽

pp. 139-144 ◽

Cited By ~ 3

Author(s):

Jayasri Ghose-Dastidar ◽

Reza Green ◽

J.B. Alexander Ross

Keyword(s):

Chemical Modification ◽

Binding Site ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Specific Chemical ◽

Site Specific ◽

Corticosteroid Binding Globulin ◽

Specific Chemical Modification ◽

Steroid Binding

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Location of the Catalytic Site for Phosphoenolpyruvate Formation within the Primary Structure of Clostridium symbiosum Pyruvate Phosphate Dikinase. 1. Identification of an Essential Cysteine by Chemical Modification with [1-14C]Bromopyruvate and Site-Directed Mutagenesis

Biochemistry ◽

10.1021/bi00007a011 ◽

1995 ◽

Vol 34 (7) ◽

pp. 2181-2187 ◽

Cited By ~ 14

Author(s):

Yuan Xu ◽

Linda Yankie ◽

Li Shen ◽

Young-Shik Jung ◽

Patrick S. Mariano ◽

...

Keyword(s):

Chemical Modification ◽

Primary Structure ◽

Catalytic Site ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Pyruvate Phosphate Dikinase ◽

Clostridium Symbiosum

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Mechanistic Studies on CDP-6-deoxy-.DELTA.3,4-glucoseen Reductase: The Role of Cysteine Residues in Catalysis As Probed by Chemical Modification and Site-Directed Mutagenesis

Biochemistry ◽

10.1021/bi00013a003 ◽

1995 ◽

Vol 34 (13) ◽

pp. 4159-4168 ◽

Cited By ~ 15

Author(s):

Olivier Ploux ◽

Yenyoung Lei ◽

Kirsi Vatanen ◽

Hung-wen Liu

Keyword(s):

Chemical Modification ◽

Site Directed Mutagenesis ◽

Cysteine Residues ◽

Directed Mutagenesis ◽

Mechanistic Studies

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Insights into the reaction mechanism of the diisopropyl fluorophosphatase from Loligo vulgaris by means of kinetic studies, chemical modification and site-directed mutagenesis

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology ◽

10.1016/s0167-4838(01)00153-4 ◽

2001 ◽

Vol 1546 (2) ◽

pp. 312-324 ◽

Cited By ~ 35

Author(s):

Judith Hartleib ◽

Heinz Rüterjans

Keyword(s):

Reaction Mechanism ◽

Chemical Modification ◽

Kinetic Studies ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Loligo Vulgaris

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A combination of site-directed mutagenesis and chemical modification to improve diastereopreference of Pseudomonas alcaligenes lipase

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics ◽

10.1016/j.bbapap.2013.08.011 ◽

2013 ◽

Vol 1834 (12) ◽

pp. 2494-2501 ◽

Cited By ~ 10

Author(s):

Hui Chen ◽

Jianping Wu ◽

Lirong Yang ◽

Gang Xu

Keyword(s):

Chemical Modification ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Pseudomonas Alcaligenes

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Characterization of the 2-[(R)-2-Hydroxypropylthio]ethanesulfonate Dehydrogenase fromXanthobacterStrain Py2: Product Inhibition, pH Dependence of Kinetic Parameters, Site-Directed Mutagenesis, Rapid Equilibrium Inhibition, and Chemical Modification†

Biochemistry ◽

10.1021/bi0118005 ◽

2002 ◽

Vol 41 (8) ◽

pp. 2727-2740 ◽

Cited By ~ 10

Author(s):

Daniel D. Clark ◽

Scott A. Ensign

Keyword(s):

Chemical Modification ◽

Kinetic Parameters ◽

Ph Dependence ◽

Product Inhibition ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Rapid Equilibrium

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