scholarly journals Stereospecific Synthesis of 23-Hydroxyundecylprodiginines and Analogues and Conversion to Antimalarial Premarineosins via a Rieske Oxygenase Catalyzed Bicyclization

2014 ◽  
Vol 79 (23) ◽  
pp. 11674-11689 ◽  
Author(s):  
Papireddy Kancharla ◽  
Wanli Lu ◽  
Shaimaa M. Salem ◽  
Jane Xu Kelly ◽  
Kevin A. Reynolds
Keyword(s):  
Stereospecific Synthesis ◽  
Rieske Oxygenase

Stereospecific Synthesis of m-Hydroxymexiletine Enantiomers

2013 ◽  
Vol 6 (3) ◽  
pp. 182-186 ◽  
Author(s):  
Alessia Catalano ◽  
Alessia Carocci ◽  
Giovanni Lentini ◽  
Ivana Defrenza ◽  
Claudio Bruno ◽  
...  
Keyword(s):  
Stereospecific Synthesis

Stereospecific synthesis of functionalized cyclopentanes

1989 ◽  
Vol 30 (42) ◽  
pp. 5803-5806 ◽  
Author(s):  
Alfred Hassner ◽  
Rakesh Maurya
Keyword(s):  
Stereospecific Synthesis

scholarly journals Further Studies on the 3-Ketosteroid 9α-Hydroxylase of Rhodococcus ruber Chol-4, a Rieske Oxygenase of the Steroid Degradation Pathway

2021 ◽  
Vol 9 (6) ◽  
pp. 1171
Author(s):  
Sara Baldanta ◽  
Juana María Navarro Llorens ◽  
Govinda Guevara
Keyword(s):  
Cholesterol Metabolism ◽  
Genetic Regulation ◽  
Degradation Pathway ◽  
Rhodococcus Ruber ◽  
Promoter Regions ◽  
Steroid Nucleus ◽  
Steroid Substrate ◽  
Fine Regulation ◽  
Rieske Oxygenase ◽  
Steroid Catabolism

The biochemistry and genetics of the bacterial steroid catabolism have been extensively studied during the last years and their findings have been essential to the development of biotechnological applications. For instance, metabolic engineering of the steroid-eater strains has allowed to obtain intermediaries of industrial value. However, there are still some drawbacks that must be overcome, such as the redundancy of the steroid catabolism genes in the genome and a better knowledge of its genetic regulation. KshABs and KstDs are key enzymes involved in the aerobic breakage of the steroid nucleus. Rhodococcus ruber Chol-4 contains three kshAs genes, a single kshB gene and three kstDs genes within its genome. In the present work, the growth of R. ruber ΔkshA strains was evaluated on different steroids substrates; the promoter regions of these genes were analyzed; and their expression was followed by qRT-PCR in both wild type and ksh mutants. Additionally, the transcription level of the kstDs genes was studied in the ksh mutants. The results show that KshA2B and KshA1B are involved in AD metabolism, while KshA3B and KshA1B contribute to the cholesterol metabolism in R. ruber. In the kshA single mutants, expression of the remaining kshA and kstD genes is re-organized to survive on the steroid substrate. These data give insight into the fine regulation of steroid genes when several isoforms are present.

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