scholarly journals Site-Selective, Cleavable Linkers: Quality Control and the Characterization of Small Molecules on Microelectrode Arrays

2011 ◽  
Vol 76 (21) ◽  
pp. 9053-9059 ◽  
Author(s):  
Bo Bi ◽  
Richard Y.-C. Huang ◽  
Karl Maurer ◽  
Ceng Chen ◽  
Kevin D. Moeller
Keyword(s):  
Quality Control ◽  
Small Molecules ◽  
Microelectrode Arrays ◽  
Site Selective

Systematic Engineering of a Protein Nanocage for High-Yield, Site-Specific Modification

2018 ◽  
Author(s):  
Daniel D. Brauer ◽  
Emily C. Hartman ◽  
Daniel L.V. Bader ◽  
Zoe N. Merz ◽  
Danielle Tullman-Ercek ◽  
...  
Keyword(s):  
Small Molecules ◽  
Protein Modification ◽  
Positive Charge ◽  
Specific Protein ◽  
High Yield ◽  
Site Specific ◽  
Protein Cage ◽  
Protein Carriers ◽  
Site Selective ◽  
Targeted Library

<div> <p>Site-specific protein modification is a widely-used strategy to attach drugs, imaging agents, or other useful small molecules to protein carriers. N-terminal modification is particularly useful as a high-yielding, site-selective modification strategy that can be compatible with a wide array of proteins. However, this modification strategy is incompatible with proteins with buried or sterically-hindered N termini, such as virus-like particles like the well-studied MS2 bacteriophage coat protein. To assess VLPs with improved compatibility with these techniques, we generated a targeted library based on the MS2-derived protein cage with N-terminal proline residues followed by three variable positions. We subjected the library to assembly, heat, and chemical selections, and we identified variants that were modified in high yield with no reduction in thermostability. Positive charge adjacent to the native N terminus is surprisingly beneficial for successful extension, and over 50% of the highest performing variants contained positive charge at this position. Taken together, these studies described nonintuitive design rules governing N-terminal extensions and identified successful extensions with high modification potential.</p> </div>

Emerging Techniques for the Characterization of Nano-Mechanical Properties of Integrated Circuit Components

2008 ◽  
Author(s):  
Nicholas Randall ◽  
Rahul Premachandran Nair
Keyword(s):  
Mechanical Properties ◽  
Quality Control ◽  
Integrated Circuits ◽  
Integrated Circuit ◽  
Manufacturing Process ◽  
Solder Bumps ◽  
Initial Work ◽  
Circuit Components ◽  
Materials Used

Abstract With the growing complexity of integrated circuits (IC) comes the issue of quality control during the manufacturing process. In order to avoid late realization of design flaws which could be very expensive, the characterization of the mechanical properties of the IC components needs to be carried out in a more efficient and standardized manner. The effects of changes in the manufacturing process and materials used on the functioning and reliability of the final device also need to be addressed. Initial work on accurately determining several key mechanical properties of bonding pads, solder bumps and coatings using a combination of different methods and equipment has been summarized.

Preparation and characterization of newly developed matrix using functional γ -Fe2 O3 nanoparticles for mass spectrometry in small molecules

2016 ◽  
Vol 48 (11) ◽  
pp. 1127-1131 ◽  
Author(s):  
Shota Morimoto ◽  
Tomoya Ishikawa ◽  
Kuminori Hyodo ◽  
Takahiro Yamazaki ◽  
Shu Taira ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Small Molecules

Using radiocarbon: an update

Antiquity ◽  
1994 ◽  
Vol 68 (261) ◽  
pp. 838-843 ◽  
Author(s):  
Sheridan Bowman
Keyword(s):  
Quality Control ◽  
Error Term

A note in the 1990 ANTIQUITY volume dealt with four issues crucial to the successful use of radiocarbon in archaeology (Bowman & Balaam 1990): selection and characterization of material and context; determination of the radiocarbon result and error term; interpretation and publication; and strategic resourcing. Since then much has been published, particularly on quality control of radiocarbon measurements (‘determination’), and on the calibration of radiocarbon results (‘interpretation’). Here is an update.

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