scholarly journals Synthesis of 2′-O-[2-(N-Methylcarbamoyl)ethyl]ribonucleosides Using Oxa-Michael Reaction and Chemical and Biological Properties of Oligonucleotide Derivatives Incorporating These Modified Ribonucleosides

2011 ◽  
Vol 76 (9) ◽  
pp. 3042-3053 ◽  
Author(s):  
Takeshi Yamada ◽  
Natsuki Okaniwa ◽  
Hisao Saneyoshi ◽  
Akihiro Ohkubo ◽  
Kohji Seio ◽  
...  
Keyword(s):  
Michael Reaction ◽  
Biological Properties ◽  
Oligonucleotide Derivatives

scholarly journals Cationic oligonucleotide derivatives and conjugates: A favorable approach for enhanced DNA and RNA targeting oligonucleotides

2021 ◽  
Vol 17 ◽  
pp. 1828-1848
Author(s):  
Mathias B Danielsen ◽  
Jesper Wengel
Keyword(s):  
Nucleic Acid ◽  
Conventional Medicine ◽  
Biological Properties ◽  
Cell Uptake ◽  
Future Design ◽  
Dna And Rna ◽  
Rna Targeting ◽  
Nuclease Resistance ◽  
Amine Groups ◽  
Oligonucleotide Derivatives

Antisense oligonucleotides (ASOs) have the ability of binding to endogenous nucleic acid targets, thereby inhibiting the gene expression. Although ASOs have great potential in the treatment of many diseases, the search for favorable toxicity profiles and distribution has been challenging and consequently impeded the widespread use of ASOs as conventional medicine. One strategy that has been employed to optimize the delivery profile of ASOs, is the functionalization of ASOs with cationic amine groups, either by direct conjugation onto the sugar, nucleobase or internucleotide linkage. The introduction of these positively charged groups has improved properties like nuclease resistance, increased binding to the nucleic acid target and improved cell uptake for oligonucleotides (ONs) and ASOs. The modifications highlighted in this review are some of the most prevalent cationic amine groups which have been attached as single modifications onto ONs/ASOs. The review has been separated into three sections, nucleobase, sugar and backbone modifications, highlighting what impact the cationic amine groups have on the ONs/ASOs physiochemical and biological properties. Finally, a concluding section has been added, summarizing the important knowledge from the three chapters, and examining the future design for ASOs.

Three Dimensional structure and organization of diploid chromosomes by optical section microscopy

1987 ◽  
Vol 45 ◽  
pp. 633-635
Author(s):  
David A. Agard ◽  
Yasushi Hiraoka ◽  
John W. Sedat
Keyword(s):  
Dimensional Space ◽  
Three Dimensional ◽  
Developmental State ◽  
Mitotic Cell ◽  
Biological Properties ◽  
Dimensional Structure ◽  
Specific Gene ◽  
Three Dimensional Structure ◽  
Complementary Techniques ◽  
Dynamic Structures

In an effort to understand the complex relationship between structure and biological function within the nucleus, we have embarked on a program to examine the three-dimensional structure and organization of Drosophila melanogaster embryonic chromosomes. Our overall goal is to determine how DNA and proteins are organized into complex and highly dynamic structures (chromosomes) and how these chromosomes are arranged in three dimensional space within the cell nucleus. Futher, we hope to be able to correlate structual data with such fundamental biological properties as stage in the mitotic cell cycle, developmental state and transcription at specific gene loci.Towards this end, we have been developing methodologies for the three-dimensional analysis of non-crystalline biological specimens using optical and electron microscopy. We feel that the combination of these two complementary techniques allows an unprecedented look at the structural organization of cellular components ranging in size from 100A to 100 microns.

Membrane–cytoskeleton interactions in cholesterol-dependent domain formation

2015 ◽  
Vol 57 ◽  
pp. 177-187 ◽  
Author(s):  
Jennifer N. Byrum ◽  
William Rodgers
Keyword(s):  
Biological Properties ◽  
Membrane Rafts ◽  
Membrane Domains ◽  
Biological Functions ◽  
Membrane Cytoskeleton ◽  
Heterogeneous Structures ◽  
Integral Role ◽  
Model Cell ◽  
Actomyosin Cytoskeleton ◽  
Dependent Domain

Since the inception of the fluid mosaic model, cell membranes have come to be recognized as heterogeneous structures composed of discrete protein and lipid domains of various dimensions and biological functions. The structural and biological properties of membrane domains are represented by CDM (cholesterol-dependent membrane) domains, frequently referred to as membrane ‘rafts’. Biological functions attributed to CDMs include signal transduction. In T-cells, CDMs function in the regulation of the Src family kinase Lck (p56lck) by sequestering Lck from its activator CD45. Despite evidence of discrete CDM domains with specific functions, the mechanism by which they form and are maintained within a fluid and dynamic lipid bilayer is not completely understood. In the present chapter, we discuss recent advances showing that the actomyosin cytoskeleton has an integral role in the formation of CDM domains. Using Lck as a model, we also discuss recent findings regarding cytoskeleton-dependent CDM domain functions in protein regulation.

Biological properties of the berries of five Juniperus species in Juniperus section from Turkey

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
N Miceli ◽  
MF Taviano ◽  
A Trovato ◽  
R De Pasquale ◽  
P Maimone ◽  
...  
Keyword(s):  
Biological Properties

Chemical composition and biological properties of Piper cabralanum C. DC. (Piperaceae)

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
D Moreira ◽  
F Candido ◽  
M Siqueira ◽  
C Quaresma ◽  
E Guimarâes ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Biological Properties

Characterization of Portuguese Propolis: Unraveling Biological Properties

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
CA Aguiar ◽  
AM Ferreira ◽  
R Oliveira ◽  
F Baltazar ◽  
A Cunha
Keyword(s):  
Biological Properties

Chemical constituents and biological properties of liverworts from South Africa

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
S Combrinck ◽  
J Linde ◽  
A Ludwiczuk ◽  
S Van Vuuren ◽  
J Van Rooy ◽  
...  
Keyword(s):  
South Africa ◽  
Chemical Constituents ◽  
Biological Properties

COUNTING TOTAL BACTERIA IN LAND ORGANIC WASTE HOUSEHOLD AND LAND INORGANIC WITH TOTAL PLATE COUNT METHOD (TPC)

2017 ◽  
Vol 4 (2) ◽  
pp. 87-91
Author(s):  
Ekamaida Ekamaida
Keyword(s):  
Soil Fertility ◽  
Bacterial Population ◽  
Organic Soil ◽  
Biological Properties ◽  
Plate Count ◽  
Bacterial Populations ◽  
Fertility Data ◽  
Plate Count Method ◽  
The Mean ◽  
Total Bacteria

The soil fertility aspect is characterized by the good biological properties of the soil. One important element of the soil biological properties is the bacterial population present in it. This research was conducted in the laboratory of Microbiology University of Malikussaleh in the May until June 2016. This study aims to determine the number of bacterial populations in soil organic and inorganic so that can be used as an indicator to know the level of soil fertility. Data analysis was done by T-Test that is by comparing the mean of observation parameter to each soil sample. The sampling method used is a composite method, which combines 9 of soil samples taken from 9 sample points on the same plot diagonally both on organic soil and inorganic soil. The results showed the highest bacterial population was found in total organic soil cfu 180500000 and total inorganic soil cfu 62.500.000

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