Versatile Site-Specific Conjugation of Small Molecules to siRNA Using Click Chemistry

2011 ◽  
Vol 76 (5) ◽  
pp. 1198-1211 ◽  
Author(s):  
Takeshi Yamada ◽  
Chang Geng Peng ◽  
Shigeo Matsuda ◽  
Haripriya Addepalli ◽  
K. Narayanannair Jayaprakash ◽  
...  
Keyword(s):  
Small Molecules ◽  
Click Chemistry ◽  
Site Specific

Systematic Engineering of a Protein Nanocage for High-Yield, Site-Specific Modification

2018 ◽  
Author(s):  
Daniel D. Brauer ◽  
Emily C. Hartman ◽  
Daniel L.V. Bader ◽  
Zoe N. Merz ◽  
Danielle Tullman-Ercek ◽  
...  
Keyword(s):  
Small Molecules ◽  
Protein Modification ◽  
Positive Charge ◽  
Specific Protein ◽  
High Yield ◽  
Site Specific ◽  
Protein Cage ◽  
Protein Carriers ◽  
Site Selective ◽  
Targeted Library

<div> <p>Site-specific protein modification is a widely-used strategy to attach drugs, imaging agents, or other useful small molecules to protein carriers. N-terminal modification is particularly useful as a high-yielding, site-selective modification strategy that can be compatible with a wide array of proteins. However, this modification strategy is incompatible with proteins with buried or sterically-hindered N termini, such as virus-like particles like the well-studied MS2 bacteriophage coat protein. To assess VLPs with improved compatibility with these techniques, we generated a targeted library based on the MS2-derived protein cage with N-terminal proline residues followed by three variable positions. We subjected the library to assembly, heat, and chemical selections, and we identified variants that were modified in high yield with no reduction in thermostability. Positive charge adjacent to the native N terminus is surprisingly beneficial for successful extension, and over 50% of the highest performing variants contained positive charge at this position. Taken together, these studies described nonintuitive design rules governing N-terminal extensions and identified successful extensions with high modification potential.</p> </div>

A modified dinucleotide for site-specific RNA-labelling by transcription priming and click chemistry

2014 ◽  
Vol 50 (11) ◽  
pp. 1313-1316 ◽  
Author(s):  
Ayan Samanta ◽  
André Krause ◽  
Andres Jäschke
Keyword(s):  
Click Chemistry ◽  
Site Specific

scholarly journals Peptide-tags for site-specific protein labelling in vitro and in vivo

2016 ◽  
Vol 12 (6) ◽  
pp. 1731-1745 ◽  
Author(s):  
Jonathan Lotze ◽  
Ulrike Reinhardt ◽  
Oliver Seitz ◽  
Annette G. Beck-Sickinger
Keyword(s):  
Metal Ions ◽  
Small Molecules ◽  
Protein Localization ◽  
Specific Protein ◽  
Site Specific ◽  
Protein Labelling ◽  
Peptide Interactions ◽  
Peptide Tags

Peptide-tag based labelling can be achieved by (i) enzymes (ii) recognition of metal ions or small molecules and (iii) peptide–peptide interactions and enables site-specific protein visualization to investigate protein localization and trafficking.

Site-Specific Surface Functionalization via Microchannel Cantilever Spotting (µCS): Comparison between Azide-Alkyne and Thiol-Alkyne Click Chemistry Reactions

Small ◽  
2018 ◽  
Vol 14 (21) ◽  
pp. 1800131 ◽  
Author(s):  
Seyed Mohammad Mahdi Dadfar ◽  
Sylwia Sekula-Neuner ◽  
Uwe Bog ◽  
Vanessa Trouillet ◽  
Michael Hirtz
Keyword(s):  
Specific Surface ◽  
Click Chemistry ◽  
Surface Functionalization ◽  
Site Specific

Polymer Pen Lithography (PPL)-Induced Site-Specific Click Chemistry for the Formation of Functional Glycan Arrays

Small ◽  
2012 ◽  
Vol 8 (13) ◽  
pp. 2000-2005 ◽  
Author(s):  
Shudan Bian ◽  
Jiajun He ◽  
Kevin B. Schesing ◽  
Adam B. Braunschweig
Keyword(s):  
Click Chemistry ◽  
Site Specific ◽  
Polymer Pen Lithography

A Noncanonical Function of Sortase Enables Site-Specific Conjugation of Small Molecules to Lysine Residues in Proteins

2014 ◽  
Vol 127 (2) ◽  
pp. 451-455 ◽  
Author(s):  
Joseph J. Bellucci ◽  
Jayanta Bhattacharyya ◽  
Ashutosh Chilkoti
Keyword(s):  
Small Molecules ◽  
Site Specific ◽  
Lysine Residues

scholarly journals Site‐specific Bioconjugation and Convergent Click Chemistry Enhances Antibody–Chromophore Conjugate Binding Efficiency

2020 ◽  
Vol 96 (3) ◽  
pp. 596-603 ◽  
Author(s):  
Amissi Sadiki ◽  
Eric M. Kercher ◽  
Haibin Lu ◽  
Ryan T. Lang ◽  
Bryan Q. Spring ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Site Specific

scholarly journals Antibiotic Spider Silk: Site-Specific Functionalization of Recombinant Spider Silk Using “Click” Chemistry

2016 ◽  
Vol 29 (10) ◽  
pp. 1604245 ◽  
Author(s):  
David Harvey ◽  
Philip Bardelang ◽  
Sara L. Goodacre ◽  
Alan Cockayne ◽  
Neil R. Thomas
Keyword(s):  
Click Chemistry ◽  
Spider Silk ◽  
Site Specific

scholarly journals Synthetic Elaboration of Native DNA by RASS (SENDR)

2020 ◽  
Author(s):  
Dillon T. Flood ◽  
Kyle W. Knouse ◽  
Julien C. Vantourout ◽  
Brittany Sanchez ◽  
Emily J. Sturgell ◽  
...  
Keyword(s):  
Small Molecules ◽  
Solid Support ◽  
High Yield ◽  
Organic Media ◽  
Two Stage ◽  
Site Specific ◽  
Reversible Adsorption ◽  
Dna Conformations ◽  
Single Coupling ◽  
Stage Process

The controlled, site-specific ligation of molecules to native DNA remains an unanswered challenge. Herein, we report a simple solution to achieve this ligation through the tactical combination of two recently developed technologies: One for the manipulation of DNA in organic media, and another for the chemoselective labeling of alcohols. Reversible Adsorption of Solid Support (RASS) is employed to immobilize DNA and facilitate its transfer into dry acetonitrile. Subsequent ligation with P(V)-based Ψ reagents takes place in high yield with exquisite selectivity for the exposed 3’ or 5’ alcohols on DNA. This two-stage process, dubbed SENDR for Synthetic Elaboration of Native DNA by RASS, can be applied to a multitude of DNA conformations and sequences with a variety of functionalized Ψ reagents to generate useful constructs. Such entities can address numerous longstanding challenges, including the selective single coupling of DNA to proteins, ASOs, and functional small molecules, and also can allow the synthesis of doubly-labeled congeners for novel probe constructs including ones of potential interest to COVID-19 research. Finally, a prototype for the industrialization of SENDR in a kit format is presented.

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