scholarly journals Stereoselective Synthesis of Neu5Acα(2→5)Neu5Gc:  The Building Block of Oligo/Poly(→5-OglycolylNeu5Gcα2→) Chains in Sea Urchin Egg Cell Surface Glycoprotein

2002 ◽  
Vol 67 (21) ◽  
pp. 7565-7568 ◽  
Author(s):  
Gang-Ting Fan ◽  
Chen-Chang Lee ◽  
Chun-Cheng Lin ◽  
Jim-Min Fang
Keyword(s):  
Cell Surface ◽  
Sea Urchin ◽  
Building Block ◽  
Stereoselective Synthesis ◽  
Surface Glycoprotein ◽  
Egg Cell ◽  
Cell Surface Glycoprotein ◽  
Sea Urchin Egg

scholarly journals The Occurrence of Novel 9-O-SulfatedN-Glycolylneuraminic Acid-capped 25-O-linked Oligo/PolyNeu5Gc Chains in Sea Urchin Egg Cell Surface Glycoprotein

1996 ◽  
Vol 271 (12) ◽  
pp. 6694-6701 ◽  
Author(s):  
Shinobu Kitazume ◽  
Ken Kitajima ◽  
Sadako Inoue ◽  
Stuart M. Haslam ◽  
Howard R. Morris ◽  
...  
Keyword(s):  
Cell Surface ◽  
Sea Urchin ◽  
Surface Glycoprotein ◽  
Egg Cell ◽  
Cell Surface Glycoprotein ◽  
Sea Urchin Egg

scholarly journals Identification of the sea urchin egg receptor for sperm using an antiserum raised against a fragment of its extracellular domain.

1992 ◽  
Vol 116 (3) ◽  
pp. 647-658 ◽  
Author(s):  
K R Foltz ◽  
W J Lennarz
Keyword(s):  
Cell Surface ◽  
Sea Urchin ◽  
Egg Cell ◽  
Cortical Granule ◽  
Dependent Manner ◽  
Western Blots ◽  
Cell Surface Glycoprotein ◽  
Sperm Binding ◽  
Sea Urchin Egg ◽  
Species Specific

Sea urchin egg fertilization requires the species-specific interaction of molecules on the sperm and egg surfaces. Previously, we isolated an extracellular, 70-kD glycosylated fragment of the S. purpuratus egg receptor for sperm by treating the eggs with lysylendoproteinase C (Foltz, K. R., and W. J. Lennarz. 1990. J. Cell Biol. 111:2951-2959). To characterize the receptor further, we have generated a polyclonal antiserum (anti-70KL) against the purified 70-kD fragment. Anti-70KL was found to react with a single polypeptide of approximately 350 kD on Western blots, presumed to be the intact receptor, in an egg cell surface preparation. This polypeptide appeared to be tightly associated with the plasma membrane/vitelline layer complex, as it was released from these preparations only by detergent treatment. Immunofluorescence microscopy revealed that the receptor was distributed evenly over the egg surface. The anti-70KL was species specific both in its ability to recognize the egg surface protein and to inhibit sperm binding. Fab fragments generated from affinity-purified anti-70KL also bound to the egg surface and inhibited sperm binding in a concentration-dependent manner. Interestingly, treatment with Fabs caused a small percentage of eggs to undergo cortical granule exocytosis, even in the absence of external Ca2+. These results confirm earlier findings indicating that the receptor is a cell surface glycoprotein of high molecular weight that species specifically binds sperm. This antiserum provides a powerful tool for further investigation of gamete interactions and the structure of the sperm receptor.

Developmental distribution of a cell surface glycoprotein in the sea urchin Strongylocentrotus purpuratus

1988 ◽  
Vol 129 (2) ◽  
pp. 339-349 ◽  
Author(s):  
Glenn L. Decker ◽  
Maria C. Valdizan ◽  
Gary M. Wessel ◽  
William J. Lennarz
Keyword(s):  
Cell Surface ◽  
Sea Urchin ◽  
Strongylocentrotus Purpuratus ◽  
Surface Glycoprotein ◽  
Cell Surface Glycoprotein ◽  
A Cell

Stereoselective synthesis of a 9-O-sulfo Neu5Gc-capped O-linked oligosaccharide found on the sea urchin egg receptor

2019 ◽  
Vol 6 (1) ◽  
pp. 54-61 ◽  
Author(s):  
Anindya Das ◽  
Pei-Jhen Li ◽  
Avijit K. Adak ◽  
Hsin-Ru Wu ◽  
Mohammad Tarique Anwar ◽  
...  
Keyword(s):  
Total Synthesis ◽  
Sea Urchin ◽  
Stereoselective Synthesis ◽  
Egg Cell ◽  
Cell Surfaces ◽  
Sea Urchin Egg

The first total synthesis of a serine bearing α2→5-Oglycolyl-linked oligoNeu5Gc found on sea urchin egg cell surfaces has been accomplished.

Immunogold and immunoblot studies on a cell surface protein found in primary mesenchyme cells and in the cortical secretory vesicles of the sea urchin egg

1987 ◽  
Vol 45 ◽  
pp. 832-833
Author(s):  
G.L. Decker ◽  
M.C. Valdizan
Keyword(s):  
Cell Surface ◽  
Sea Urchin ◽  
Surface Protein ◽  
Egg Cell ◽  
Secretory Vesicles ◽  
Cell Surface Protein ◽  
Surface Complexes ◽  
Mesenchyme Cells ◽  
Lowicryl K4m ◽  
Primary Mesenchyme Cells

A monoclonal antibody designated MAb 1223 has been used to show that primary mesenchyme cells of the sea urchin embryo express a 130-kDa cell surface protein that may be directly involved in Ca2+ uptake required for growth of skeletal spicules. Other studies from this laboratory have shown that the 1223 antigen, although in relatively low abundance, is also expressed on the cell surfaces of unfertilized eggs and on the majority of blastomeres formed prior to differentiation of the primary mesenchyme cells.We have studied the distribution of 1223 antigen in S. purpuratus eggs and embryos and in isolated egg cell surface complexes that contain the cortical secretory vesicles. Specimens were fixed in 1.0% paraformaldehyde and 1.0% glutaraldehyde and embedded in Lowicryl K4M as previously reported. Colloidal gold (8nm diameter) was prepared by the method of Mulpfordt.

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