Building Blocks for the Solution Phase Synthesis of Oligonucleotides:  Regioselective Hydrolysis of 3‘,5‘-Di-O-levulinylnucleosides Using an Enzymatic Approach

2002 ◽  
Vol 67 (13) ◽  
pp. 4513-4519 ◽  
Author(s):  
Javier García ◽  
Susana Fernández ◽  
Miguel Ferrero ◽  
Yogesh S. Sanghvi ◽  
Vicente Gotor
Keyword(s):  
Building Blocks ◽  
Solution Phase ◽  
Phase Synthesis ◽  
Solution Phase Synthesis ◽  
Hydrolysis Of ◽  
Regioselective Hydrolysis

scholarly journals Total synthesis of TMG-chitotriomycin based on an automated electrochemical assembly of a disaccharide building block

2017 ◽  
Vol 13 ◽  
pp. 919-924 ◽  
Author(s):  
Yuta Isoda ◽  
Norihiko Sasaki ◽  
Kei Kitamura ◽  
Shuji Takahashi ◽  
Sujit Manmode ◽  
...  
Keyword(s):  
Total Synthesis ◽  
Building Block ◽  
Building Blocks ◽  
Solution Phase ◽  
Protecting Groups ◽  
Phase Synthesis ◽  
Solution Phase Synthesis

The total synthesis of TMG-chitotriomycin using an automated electrochemical synthesizer for the assembly of carbohydrate building blocks is demonstrated. We have successfully prepared a precursor of TMG-chitotriomycin, which is a structurally-pure tetrasaccharide with typical protecting groups, through the methodology of automated electrochemical solution-phase synthesis developed by us. The synthesis of structurally well-defined TMG-chitotriomycin has been accomplished in 10-steps from a disaccharide building block.

scholarly journals Preparation of a disulfide-linked precipitative soluble support for solution-phase synthesis of trimeric oligodeoxyribonucleotide 3´-(2-chlorophenylphosphate) building blocks

2015 ◽  
Vol 11 ◽  
pp. 1553-1560 ◽  
Author(s):  
Amit M Jabgunde ◽  
Alejandro Gimenez Molina ◽  
Pasi Virta ◽  
Harri Lönnberg
Keyword(s):  
Disulfide Bond ◽  
Building Blocks ◽  
Reductive Cleavage ◽  
Solution Phase ◽  
Phase Synthesis ◽  
Solution Phase Synthesis

The preparation of a disulfide-tethered precipitative soluble support and its use for solution-phase synthesis of trimeric oligodeoxyribonucleotide 3´-(2-chlorophenylphosphate) building blocks is described. To obtain the building blocks, N-acyl protected 2´-deoxy-5´-O-(4,4´-dimethoxytrityl)ribonucleosides were phosphorylated with bis(benzotriazol-1-yl) 2-chlorophenyl phosphate. The “outdated” phosphotriester strategy, based on coupling of PV building blocks in conjunction with quantitative precipitation of the oligodeoxyribonucleotide with MeOH is applied. Subsequent release of the resulting phosphate and base-protected oligodeoxyribonucleotide trimer 3’-pTpdCBzpdGibu-5’ as its 3’-(2-chlorophenyl phosphate) was achieved by reductive cleavage of the disulfide bond.

scholarly journals Solution Phase Synthesis and Workup Using an Integrated Approach

2003 ◽  
Vol 8 (2) ◽  
pp. 41-43
Author(s):  
Lewin T. Wint ◽  
Marija Kovacevic ◽  
Matt Waters ◽  
Mettler-Toledo AutoChem
Keyword(s):  
Organic Synthesis ◽  
Integrated Approach ◽  
Building Blocks ◽  
Solution Phase ◽  
Automated System ◽  
Automated Systems ◽  
Time Saving ◽  
Phase Synthesis ◽  
Drug Candidates ◽  
Solution Phase Synthesis

Traditional organic synthesis is often cumbersome and time consuming. Significant effort by manufacturers of automated systems has been directed at increasing the speed, efficiency, and consistency of performing chemical reactions. To date, the numbers of integrated systems that combine all the relevant steps of compound preparation are few. Using the five automated systems in tandem can successfully streamline research and development of potential drug candidates. The features of these workstations offer a unique approach for supporting the convenient synthesis and workup of diverse compounds without compromising reagent types or conditions used for synthesis. This poster details an example of the automated drug discovery approach to high throughput organic synthesis using the solution phase synthesis of a series of 1-indanones as building blocks and subsequent reductive amination reactions to generate a diverse group of amines. The purpose of each automated system is outlined along with its role in generating the small library as an illustration of the importance of time saving devices in laboratories.

scholarly journals Solution phase synthesis of short oligoribonucleotides on a precipitative tetrapodal support

2014 ◽  
Vol 10 ◽  
pp. 2279-2285 ◽  
Author(s):  
Alejandro Gimenez Molina ◽  
Amit M Jabgunde ◽  
Pasi Virta ◽  
Harri Lönnberg
Keyword(s):  
Building Block ◽  
Rna Synthesis ◽  
Building Blocks ◽  
Solution Phase ◽  
Dipolar Cycloaddition ◽  
Proof Of Concept ◽  
Phase Synthesis ◽  
Solution Phase Synthesis ◽  
High Yields

An effective method for the synthesis of short oligoribonucleotides in solution has been elaborated. Novel 2'-O-(2-cyanoethyl)-5'-O-(1-methoxy-1-methylethyl) protected ribonucleoside 3'-phosphoramidites have been prepared and their usefulness as building blocks in RNA synthesis on a soluble support has been demonstrated. As a proof of concept, a pentameric oligoribonucleotide, 3'-UUGCA-5', has been prepared on a precipitative tetrapodal tetrakis(4-azidomethylphenyl)pentaerythritol support. The 3'-terminal nucleoside was coupled to the support as a 3'-O-(4-pentynoyl) derivative by Cu(I) promoted 1,3-dipolar cycloaddition. Couplings were carried out with 1.5 equiv of the building block. In each coupling cycle, the small molecular reagents and byproducts were removed by two quantitative precipitations from MeOH, one after oxidation and the second after the 5'-deprotection. After completion of the chain assembly, treatment with triethylamine, ammonia and TBAF released the pentamer in high yields.

Synthesis of Yolk-Shell Co3O4/Co1-xRuxO2 Microspheres Featuring an Enhanced Electrocatalytic Oxygen Evolution in Acidic Medium

2021 ◽  
Author(s):  
Abinaya Annamalai ◽  
Dipak Vijaykumar Shinde ◽  
Joka Buha ◽  
Sergio Marras ◽  
Mirko Prato ◽  
...  
Keyword(s):  
Water Splitting ◽  
Oxygen Evolution ◽  
Acidic Medium ◽  
Building Blocks ◽  
Solution Phase ◽  
Phase Synthesis ◽  
Hollow Structures ◽  
Solution Phase Synthesis ◽  
Electrochemical Water Splitting

Hollow structures made of nanoscale building blocks are of great interest as catalysts for electrochemical water splitting. Here we report a solution-phase synthesis of yolk-shell Co3O4/Co1-xRuxO2 microspheres (MSs) having a...

scholarly journals Three Dimensional ZnO Hierarchical Nanostructures: Solution Phase Synthesis and Applications

2017 ◽  
Author(s):  
Xiaoliang Wang ◽  
Mashkoor Ahmad ◽  
Hongyu Sun
Keyword(s):  
Three Dimensional ◽  
Hierarchical Structures ◽  
Lithium Ion ◽  
Building Blocks ◽  
Solution Phase ◽  
Personal Perspective ◽  
Zno Nanostructures ◽  
Hierarchical Nanostructures ◽  
Phase Synthesis ◽  
Solution Phase Synthesis

Zinc oxide (ZnO) nanostructures have been studied extensively in the past years due to the novel electronic, photonic, mechanical and electrochemical properties. Recently, more attention has been paid to assemble nanoscale building blocks into three dimensional (3D) complex hierarchical structures, which not only inherit the excellent properties of the single building blocks but also provide potential applications in the bottom-up fabrication of functional devices. This review article focuses on 3D ZnO hierarchical nanostructures, and summarizes major advances in the solution phase synthesis, applications in environment, and electrical/electrochemical devices. We present the principles and growth mechanisms of ZnO nanostructures via different solution methods, with an emphasis on rational control of the morphology and assembly. We then discuss the applications of 3D ZnO hierarchical nanostructures in photocatalysis, field emission, electrochemical sensor, and lithium ion batteries. Throughout the discussion, the relationship between the device performance and the microstructures of 3D ZnO hierarchical nanostructures will be highlighted. This review concludes with a personal perspective on the current challenges and future researches.

Direct Regioselective Enzymatic Acylation of Nucleosides: Building-Blocks for the Solution Phase Synthesis of Oligonucleotides

2003 ◽  
Vol 22 (5-8) ◽  
pp. 1455-1457 ◽  
Author(s):  
Javier García ◽  
Susana Fernández ◽  
Miguel Ferrero ◽  
Yogesh S. Sanghvi ◽  
Vicente Gotor
Keyword(s):  
Building Blocks ◽  
Solution Phase ◽  
Phase Synthesis ◽  
Solution Phase Synthesis ◽  
Enzymatic Acylation

Direct Regioselective Enzymatic Acylation of Nucleosides: Building-Blocks for the Solution Phase Synthesis of Oligonucleotides

ChemInform ◽  
2004 ◽  
Vol 35 (8) ◽  
Author(s):  
Javier Garcia ◽  
Susana Fernandez ◽  
Miguel Ferrero ◽  
Yogesh S. Sanghvi ◽  
Vicente Gotor
Keyword(s):  
Building Blocks ◽  
Solution Phase ◽  
Phase Synthesis ◽  
Solution Phase Synthesis ◽  
Enzymatic Acylation
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