Investigation of Synthetic Hosts That Model Cation-π Sites Found at Protein Binding Domains

2002 ◽  
Vol 67 (7) ◽  
pp. 2160-2167 ◽  
Author(s):  
Vadims Dvornikovs ◽  
David B. Smithrud
Keyword(s):  
Protein Binding ◽  
Binding Domains ◽  
Synthetic Hosts

scholarly journals G-Protein binding domains of the angiotensin II AT1A receptors mapped with synthetic peptides selected from the receptor sequence

1998 ◽  
Vol 332 (3) ◽  
pp. 781-787 ◽  
Author(s):  
Hisashi KAI ◽  
R. Wayne ALEXANDER ◽  
Masuko USHIO-FUKAI ◽  
P. Reid LYONS ◽  
Marjorie AKERS ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Protein Binding ◽  
G Protein ◽  
G Proteins ◽  
Synthetic Peptides ◽  
Terminal Region ◽  
Functional Coupling ◽  
Intracellular Loop ◽  
Binding Domains ◽  
Gtpase Activation

The vascular angiotensin II type 1 receptor (AT1AR) is a member of the G-protein-coupled receptor superfamily. We mapped the G-protein binding domains of the AT1AR using synthetic peptides selected from the receptor sequence, which interfere with AT1AR–G-protein coupling. Membrane GTPase activity was used as a measure of the functional coupling in rat vascular smooth muscle cells. Peptides corresponding to the N-terminal region of the second intracellular loop (residues 125–137), the N-terminal region of the third intracellular loop (217–227) and the juxtamembranous region of the C-terminal tail (304–316) inhibited angiotensin II-induced GTPase activation by 30%, 30%, and 70%, respectively. The latter two domains (217–227 and 304–316) are predicted to form amphiphilic α-helices. Only the peptide representing residues 217–227 stimulated basal activity (45%). No synthetic peptide had a significant effect on either the number or the affinity of the AT1AR binding. These observations indicate that domains of the second and third regions and the cytoplasmic tail of the AT1AR interact with G-proteins, and that multiple contacts with these receptor domains may be important for binding and activation of the G-proteins.

scholarly journals Structural orientation of the unique and protein binding domains of Lck

2006 ◽  
Vol 20 (5) ◽  
Author(s):  
Meredith Leigh Beckham ◽  
Carol B. Post ◽  
Marietta L. Harrison
Keyword(s):  
Protein Binding ◽  
Structural Orientation ◽  
Binding Domains

scholarly journals Reversible Membrane Interaction of BAD Requires two C-terminal Lipid Binding Domains in Conjunction with 14-3-3 Protein Binding

2006 ◽  
Vol 281 (25) ◽  
pp. 17321-17336 ◽  
Author(s):  
Mirko Hekman ◽  
Stefan Albert ◽  
Antoine Galmiche ◽  
Ulrike E. E. Rennefahrt ◽  
Jochen Fueller ◽  
...  
Keyword(s):  
Protein Binding ◽  
Lipid Binding ◽  
Membrane Interaction ◽  
Binding Domains
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