Studies on cyclic peptides. 5. Conformation and interaction with small molecules of cyclic hexapeptides containing glutamic acid or aspartic acid residue

1976 ◽  
Vol 41 (15) ◽  
pp. 2584-2590 ◽  
Author(s):  
Toshiharu. Sugihara ◽  
Yukio. Imanishi ◽  
Toshinobu. Higashimura ◽  
Yasutsugu. Shimonishi
Keyword(s):  
Glutamic Acid ◽  
Small Molecules ◽  
Aspartic Acid ◽  
Cyclic Peptides ◽  
Aspartic Acid Residue ◽  
Cyclic Hexapeptides

scholarly journals Identification of a Glutamic Acid and an Aspartic Acid Residue Essential for Catalytic Activity of Aspergillopepsin II, a Non-pepsin Type Acid Proteinase

2000 ◽  
Vol 275 (34) ◽  
pp. 26607-26614 ◽  
Author(s):  
Xiang-Ping Huang ◽  
Naofumi Kagami ◽  
Hideshi Inoue ◽  
Masaki Kojima ◽  
Takao Kimura ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Acid Proteinase ◽  
Aspartic Acid Residue

scholarly journals Capsid assembly in a family of animal viruses primes an autoproteolytic maturation that depends on a single aspartic acid residue.

1994 ◽  
Vol 269 (18) ◽  
pp. 13680-13684
Author(s):  
A. Zlotnick ◽  
V.S. Reddy ◽  
R. Dasgupta ◽  
A. Schneemann ◽  
W.J. Ray ◽  
...  
Keyword(s):  
Aspartic Acid ◽  
Capsid Assembly ◽  
Aspartic Acid Residue

scholarly journals Dietary glutamic acid and aspartic acid as biomarkers for predicting diabetic retinopathy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
So Young Park ◽  
Jieun Kim ◽  
Jung Il Son ◽  
Sang Youl Rhee ◽  
Do-Yeon Kim ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Education Level ◽  
Screening Rate ◽  
Food Records ◽  
Acid Intake ◽  
Lower Education Level ◽  
Lower Education

AbstractThe screening rate of diabetic retinopathy (DR) is low despite the importance of early diagnosis. We investigated the predictive value of dietary glutamic acid and aspartic acid for diagnosis of DR using the Korea National Diabetes Program cohort study. The 2067 patients with type 2 diabetes without DR were included. The baseline intakes of energy, glutamic acid and aspartic acid were assessed using a 3-day food records. The risk of DR incidence based on intake of glutamic acid and aspartic acid was analyzed. The DR group was older, and had higher HbA1c, longer DM duration, lower education level and income than non-DR group (all p < 0.05). The intake of total energy, glutamic acid and aspartic acid were lower in DR group than non-DR group (p = 0.010, p = 0.025 and p = 0.042, respectively). There was no difference in the risk of developing DR according to the intake of glutamic acid and ascorbic acid. But, aspartic acid intake had a negative correlation with PDR. Hence, the intake of glutamic acid and aspartic acid did not affect in DR incidence. However, lower aspartic acid intake affected the PDR incidence.

scholarly journals Conversion of secretory proteins into membrane proteins by fusing with a glycosylphosphatidylinositol anchor signal of alkaline phosphatase

1994 ◽  
Vol 301 (2) ◽  
pp. 577-583 ◽  
Author(s):  
K Oda ◽  
J Cheng ◽  
T Saku ◽  
N Takami ◽  
M Sohda ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Endoplasmic Reticulum ◽  
Aspartic Acid ◽  
Chimeric Protein ◽  
Attachment Site ◽  
In Vitro Translation ◽  
Placental Alkaline Phosphatase ◽  
Wild Type ◽  
Aspartic Acid Residue

Placental alkaline phosphatase (PLAP) is initially synthesized as a precursor (proPLAP) with a C-terminal extension. We constructed a recombinant cDNA which encodes a chimeric protein (alpha GL-PLAP) comprising rat alpha 2u-globulin (alpha GL) and the C-terminal extension of PLAP. Two molecular species (25 kDa and 22 kDa) were expressed in the COS-1 cell transfected with the cDNA for alpha GL-PLAP. Only the 22 kDa form was labelled with both [3H]stearic acid and [3H]ethanolamine. Upon digestion with phosphatidylinositol-specific phospholipase C the 22 kDa form was released into the medium, indicating that this form is anchored on the cell surface via glycosylphosphatidylinositol (GPI). A specific IgG raised against a C-terminal nonapeptide of proPLAP precipitated the 25 kDa form but not the 22 kDa form, suggesting that the 25 kDa form is a precursor retaining the C-terminal propeptide. When a mutant alpha GL-PLAP, in which the aspartic acid residue is replaced with tryptophan at a putative cleavage/attachment site, was expressed in COS-1 cells, the 25 kDa precursor was the only form found inside the cell and retained in the endoplasmic reticulum, as judged by immunofluorescence microscopy. In vitro translation programmed with mRNAs coding for the wild-type and mutant forms of alpha GL-PLAP demonstrated that the C-terminal propeptide was cleaved from the wild-type chimeric protein, but not from the mutant one. This gave rise to the 22 kDa form attached with a GPI anchor, suggesting that GPI is covalently linked to the aspartic acid residue (Asp159) of alpha GL-PLAP. Taken together, these results indicate that the C-terminal propeptide of PLAP functions as a signal to render alpha GL a GPI-linked membrane protein in vitro and in vivo in cultured cells, and that the chimeric protein constructed in this study may be useful for elucidating the mechanism underlying the cleavage of the propeptide and attachment of GPI, which occur in the endoplasmic reticulum.

Biodegradation of copoly(L-aspartic acid/L-glutamic acid) in vitro

Biopolymers ◽  
1990 ◽  
Vol 29 (3) ◽  
pp. 549-557 ◽  
Author(s):  
Toshio Hayashi ◽  
Makoto Iwatsuki
Keyword(s):  
Glutamic Acid ◽  
Aspartic Acid

Stability of oxidized Escherichia coli thioredoxin and its dependence on protonation of the aspartic acid residue in the 26 position

Biochemistry ◽  
1993 ◽  
Vol 32 (29) ◽  
pp. 7526-7530 ◽  
Author(s):  
John E. Ladbury ◽  
Richard Wynn ◽  
Homme W. Hellinga ◽  
Julian M. Sturtevant
Keyword(s):  
Escherichia Coli ◽  
Aspartic Acid ◽  
Aspartic Acid Residue

scholarly journals Utilization in vivo of glucose and volatile fatty acids by sheep brain for the synthesis of acidic amino acids

1966 ◽  
Vol 101 (3) ◽  
pp. 591-597 ◽  
Author(s):  
R M O'Neal ◽  
R E Koeppe ◽  
E I Williams
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Specific Activity ◽  
Tricarboxylic Acid Cycle ◽  
Sheep Brain ◽  
The Brain

1. Free glutamic acid, aspartic acid, glutamic acid from glutamine and, in some instances, the glutamic acid from glutathione and the aspartic acid from N-acetyl-aspartic acid were isolated from the brains of sheep and assayed for radioactivity after intravenous injection of [2-(14)C]glucose, [1-(14)C]acetate, [1-(14)C]butyrate or [2-(14)C]propionate. These brain components were also isolated and analysed from rats that had been given [2-(14)C]propionate. The results indicate that, as in rat brain, glucose is by far the best precursor of the free amino acids of sheep brain. 2. Degradation of the glutamate of brain yielded labelling patterns consistent with the proposal that the major route of pyruvate metabolism in brain is via acetyl-CoA, and that the short-chain fatty acids enter the brain without prior metabolism by other tissue and are metabolized in brain via the tricarboxylic acid cycle. 3. When labelled glucose was used as a precursor, glutamate always had a higher specific activity than glutamine; when labelled fatty acids were used, the reverse was true. These findings add support and complexity to the concept of the metabolic; compartmentation' of the free amino acids of brain. 4. The results from experiments with labelled propionate strongly suggest that brain metabolizes propionate via succinate and that this metabolic route may be a limited but important source of dicarboxylic acids in the brain.

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