Synthesis and spectroscopic analysis of branched RNA fragments: messenger RNA splicing intermediates

1988 ◽  
Vol 53 (16) ◽  
pp. 3710-3722 ◽  
Author(s):  
Masad J. Damha ◽  
Kelvin K. Ogilvie
Keyword(s):  
Rna Splicing ◽  
Messenger Rna ◽  
Spectroscopic Analysis ◽  
Rna Fragments ◽  
Branched Rna

Time-of-flight Secondary Ion Mass Spectrometry of Branched RNA Fragrants: Messenger RNA Splicing Intermedistes

1990 ◽  
Vol 9 (3) ◽  
pp. 445-446 ◽  
Author(s):  
Francois Lafortune ◽  
Masad J. Damha ◽  
Xuejun Tang ◽  
Kenneth G. Standing ◽  
John B. Westmore ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Rna Splicing ◽  
Messenger Rna ◽  
Secondary Ion Mass Spectrometry ◽  
Time Of Flight ◽  
Ion Mass Spectrometry ◽  
Branched Rna ◽  
Secondary Ion

scholarly journals The Role of N6-Methyladenosine (m6A) Methylation Modifications in Hematological Malignancies

Cancers ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 332
Author(s):  
Yan Zhao ◽  
Hongling Peng
Keyword(s):  
Bone Marrow ◽  
Pluripotent Stem Cells ◽  
Rna Splicing ◽  
Messenger Rna ◽  
Hematologic Malignancies ◽  
Cellular Processes ◽  
Proliferation And Differentiation ◽  
Regulatory Functions ◽  
Increased Susceptibility

Epigenetics is identified as the study of heritable modifications in gene expression and regulation that do not involve DNA sequence alterations, such as DNA methylation, histone modifications, etc. Importantly, N6-methyladenosine (m6A) methylation modification is one of the most common epigenetic modifications of eukaryotic messenger RNA (mRNA), which plays a key role in various cellular processes. It can not only mediate various RNA metabolic processes such as RNA splicing, translation, and decay under the catalytic regulation of related enzymes but can also affect the normal development of bone marrow hematopoiesis by regulating the self-renewal, proliferation, and differentiation of pluripotent stem cells in the hematopoietic microenvironment of bone marrow. In recent years, numerous studies have demonstrated that m6A methylation modifications play an important role in the development and progression of hematologic malignancies (e.g., leukemia, lymphoma, myelodysplastic syndromes [MDS], multiple myeloma [MM], etc.). Targeting the inhibition of m6A-associated factors can contribute to increased susceptibility of patients with hematologic malignancies to therapeutic agents. Therefore, this review elaborates on the biological characteristics and normal hematopoietic regulatory functions of m6A methylation modifications and their role in the pathogenesis of hematologic malignancies.

scholarly journals Intron and Its Splicing Mechanism and Their Connection with Human Disease

2016 ◽  
Vol 15 (3) ◽  
pp. 307-312
Author(s):  
Mine Dosay-Akbulut
Keyword(s):  
Human Genome ◽  
Human Disease ◽  
Rna Splicing ◽  
Messenger Rna ◽  
Developmental Disorders ◽  
Regulation Of Gene Expression ◽  
Medical Science ◽  
Intronless Genes ◽  
Different Types ◽  
Extra Difficulty

In the maturation mechanism of a messenger RNA, splicing play an important role with removing the noncoding introns and ligating the coding exons. Alternative splicing (AS) gives an extra difficulty to this mechanism and to the regulation of gene expression. The possible disturbing in the alternative RNA splicing mechanism can be a reason to several diseases like cancers and neurodegenerative disorders. Intronless genes (IGs) are seen in almost 3% of the human genome. Functionality of IGs has an important role in signal transduction genes and related regulatory proteins. This diversity can be reason to IG-associated diseases, especially neuropathies, developmental disorders, and cancer. The retroelements can be seen in almost half of the human genome. The known informations indicate that insertion of retroelement into exons and introns of genes promote different types of genetic disease, including cancer. The retroelement connected mutagenesis cause to fifty different types of human disease. The molecular informations and bioinformatic analyses can be used to explain the connection with splicing mutations and genetic mechanisms of several different human disease and understanding of this mechanism play an important role in the formation of treatment programme against to these diseases.Bangladesh Journal of Medical Science Vol.15(3) 2016 p.307-312

scholarly journals Alternative Polyadenylation: a new frontier in post transcriptional regulation

2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Fanggang Ren ◽  
Na Zhang ◽  
Lan Zhang ◽  
Eric Miller ◽  
Jeffrey J. Pu
Keyword(s):  
Rna Splicing ◽  
Messenger Rna ◽  
Current Knowledge ◽  
Single Gene ◽  
Alternative Polyadenylation ◽  
Disease Diagnosis ◽  
Sequence Motif ◽  
Mrna Isoforms ◽  
Specific Sequence ◽  
Regulatory Complex

AbstractPolyadenylation of pre-messenger RNA (pre-mRNA) specific sites and termination of their downstream transcriptions are signaled by unique sequence motif structures such as AAUAAA and its auxiliary elements. Alternative polyadenylation (APA) is an important post-transcriptional regulatory mechanism that processes RNA products depending on its 3′-untranslated region (3′-UTR) specific sequence signal. APA processing can generate several mRNA isoforms from a single gene, which may have different biological functions on their target gene. As a result, cellular genomic stability, proliferation capability, and transformation feasibility could all be affected. Furthermore, APA modulation regulates disease initiation and progression. APA status could potentially act as a biomarker for disease diagnosis, severity stratification, and prognosis forecast. While the advance of modern throughout technologies, such as next generation-sequencing (NGS) and single-cell sequencing techniques, have enriched our knowledge about APA, much of APA biological process is unknown and pending for further investigation. Herein, we review the current knowledge on APA and how its regulatory complex factors (CFI/IIm, CPSF, CSTF, and RBPs) work together to determine RNA splicing location, cell cycle velocity, microRNA processing, and oncogenesis regulation. We also discuss various APA experiment strategies and the future direction of APA research.

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