Conformation−Function Relationship of Vitamin D:  Conformational Analysis Predicts Potential Side-Chain Structure

1998 ◽  
Vol 41 (9) ◽  
pp. 1467-1475 ◽  
Author(s):  
Sachiko Yamada ◽  
Keiko Yamamoto ◽  
Hiroyuki Masuno ◽  
Masateru Ohta
Keyword(s):  
Vitamin D ◽  
Conformational Analysis ◽  
Chain Structure ◽  
Side Chain ◽  
Function Relationship ◽  
Relationship Of

scholarly journals Split Enzyme-Based Biosensors for Structural Characterization of Soluble and Insoluble β-Glucans

2021 ◽  
Vol 22 (4) ◽  
pp. 1576
Author(s):  
Daisuke Yamanaka ◽  
Suzuka Kurita ◽  
Yuka Hanayama ◽  
Yoshiyuki Adachi
Keyword(s):  
Chain Structure ◽  
Side Chain ◽  
Dependent Manner ◽  
Close Proximity ◽  
Recognition Protein ◽  
Split Luciferase Complementation ◽  
Time Changes ◽  
Function Relationship ◽  
Relationship Of ◽  
Long Chain

β-Glucan is widely distributed in various plants and microorganisms and is composed of β-1,3-linked d-glucose units. It may have a branched short or long side chain of glucose units with β-1,6- or β-1,4-linkage. Numerous studies have investigated different β-glucans and revealed their bioactivities. To understand the structure-function relationship of β-glucan, we constructed a split-luciferase complementation assay for the structural analysis of long-chain β-1,6-branched β-1,3-glucan. The N- and C-terminal fragments of luciferase from deep-sea shrimp were fused to insect-derived β-1,3-glucan recognition protein and fungal endo-β-1,6-glucanase (Neg1)-derived β-1,6-glucan recognition protein, respectively. In this approach, two β-glucan recognition proteins bound to β-glucan molecules come into close proximity, resulting in the assembly of the full-length reporter enzyme and induction of transient luciferase activity, indicative of the structure of β-glucan. To test the applicability of this assay, β-glucan and two β-glucan recognition proteins were mixed, resulting in an increase in the luminescence intensity in a β-1,3-glucan with a long polymer of β-1,6-glucan in a dose-dependent manner. This simple test also allows the monitoring of real-time changes in the side chain structure and serves as a convenient method to distinguish between β-1,3-glucan and long-chain β-1,6-branched β-1,3-glucan in various soluble and insoluble β-glucans.

scholarly journals Relationship of 25-hydroxyvitamin D3 side chain structure to biological activity.

1975 ◽  
Vol 250 (1) ◽  
pp. 226-230
Author(s):  
M F Holick ◽  
M Garabedian ◽  
H K Schnoes ◽  
H F DeLuca
Keyword(s):  
Biological Activity ◽  
Chain Structure ◽  
Side Chain ◽  
Relationship Of

Potent Antagonist for the Vitamin D Receptor: Vitamin D Analogues with Simple Side Chain Structure

2010 ◽  
Vol 53 (15) ◽  
pp. 5813-5826 ◽  
Author(s):  
Yuta Sakamaki ◽  
Yuka Inaba ◽  
Nobuko Yoshimoto ◽  
Keiko Yamamoto
Keyword(s):  
Vitamin D ◽  
Vitamin D Receptor ◽  
Chain Structure ◽  
Side Chain ◽  
Vitamin D Analogues ◽  
Potent Antagonist
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